Construct: ORF TRCN0000478692
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005658.1_s317c1
- Derived from:
- ccsbBroadEn_08941
- DNA Barcode:
- CTCGTTACAATCCGGCTCGAGTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ROGDI (79641)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478692
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79641 | ROGDI | rogdi atypical leucine zipper | NM_024589.2 | 99.8% | 100% | 414G>A |
| 2 | human | 79641 | ROGDI | rogdi atypical leucine zipper | XM_006720947.4 | 97.5% | 97.2% | 414G>A;696_716del |
| 3 | human | 79641 | ROGDI | rogdi atypical leucine zipper | XM_006720948.4 | 66.8% | 66.6% | 0_1ins270;144G>A;426_446del |
| 4 | human | 79641 | ROGDI | rogdi atypical leucine zipper | NR_046480.1 | 45.3% | (many diffs) | |
| 5 | mouse | 66049 | Rogdi | rogdi homolog | NM_133185.2 | 87.9% | 94.4% | (many diffs) |
| 6 | mouse | 66049 | Rogdi | rogdi homolog | XM_017317109.1 | 66% | 70% | (many diffs) |
| 7 | mouse | 66049 | Rogdi | rogdi homolog | XM_011245993.2 | 54.9% | 57.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 927
- ORF length:
- 861
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc caccgtgatg gcagcgacgg cggcggagcg ggcggtgctg gaggaggagt 121 tccgctggct gctgcacgac gaggtgcacg ctgtgttgaa gcagctgcag gacatcctca 181 aggaggcctc tctgcgcttc actctgccgg gctccggcac tgaggggccc gccaagcaag 241 agaacttcat cctaggcagc tgtggcacag accaggtgaa gggtgtgctg actctgcagg 301 gggatgccct cagccaggcg gatgtgaacc tgaagatgcc ccggaacaac cagctgctgc 361 acttcgcctt ccgggaggac aagcagtgga agctgcagca gatccaggat gccagaaacc 421 atgtgagcca agccatttac ctgcttacca gccgggacca gagctaccag ttcaagacag 481 gcgctgaggt cctcaagctg atggacgcag tgatgctgca gctgaccaga gcccgaaacc 541 ggctcaccac ccccgccacc cTCACCCTCC CCGAGATCGC CGCCAGCGGC CTCACGCGGA 601 TGTTCGCCCC TGCCCTGCCG TCCGACCTGC TGGTCAACGT CTACATCAAC CTCAACAAGC 661 TCTGCCTCAC GGTGTACCAG CTGCATGCCC TGCAGCCCAA CTCCACCAAG AACTTCCGCC 721 CAGCTGGGGG CGCGGTGCTG CATAGCCCTG GGGCCATGTT CGAGTGGGGC TCTCAGCGCC 781 TGGAGGTGAG CCACGTGCAC AAAGTGGAGT GCGTGATCCC CTGGCTCAAC GACGCCCTGG 841 TCTACTTCAC CGTCTCCCTG CAGCTCTGCC AGCAGCTCAA GGACAAGATC TCCGTGTTCT 901 CCAGCTACTG GAGCTACAGA CCCTTCTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 961 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1021 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACTCGTTAC 1081 AATCCGGCTC GAGTACACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1141 aagatt