Construct: ORF TRCN0000478786
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001695.2_s317c1
- Derived from:
- ccsbBroadEn_01526
- DNA Barcode:
- TCTGTTATTACTGCCATCTCCATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SGCA (6442)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478786
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6442 | SGCA | sarcoglycan alpha | NM_000023.4 | 100% | 100% | |
| 2 | human | 6442 | SGCA | sarcoglycan alpha | XM_011525120.2 | 81.7% | 78.9% | (many diffs) |
| 3 | human | 6442 | SGCA | sarcoglycan alpha | XM_024450873.1 | 73.5% | 73.1% | 0_1ins301;2T>G;5_6insATTGA |
| 4 | human | 6442 | SGCA | sarcoglycan alpha | XM_011525121.2 | 71% | 68.2% | (many diffs) |
| 5 | human | 6442 | SGCA | sarcoglycan alpha | XM_011525122.2 | 67.9% | 61.1% | (many diffs) |
| 6 | human | 6442 | SGCA | sarcoglycan alpha | NM_001135697.3 | 67.9% | 67.9% | 583_584ins372 |
| 7 | human | 6442 | SGCA | sarcoglycan alpha | XM_011525124.2 | 67.6% | 64.1% | (many diffs) |
| 8 | human | 6442 | SGCA | sarcoglycan alpha | NR_135553.2 | 66.6% | 1_36del;782_783ins209;989_1220del | |
| 9 | human | 6442 | SGCA | sarcoglycan alpha | XR_002958056.1 | 58.3% | 1_518del;1265_1343del;1759_1990del | |
| 10 | human | 6442 | SGCA | sarcoglycan alpha | XM_011525123.2 | 55.2% | 52.5% | (many diffs) |
| 11 | mouse | 20391 | Sgca | sarcoglycan, alpha (dystrop... | NM_009161.4 | 84.1% | 88.8% | (many diffs) |
| 12 | mouse | 20391 | Sgca | sarcoglycan, alpha (dystrop... | XM_011248836.2 | 84.1% | 88.8% | (many diffs) |
| 13 | mouse | 20391 | Sgca | sarcoglycan, alpha (dystrop... | XM_017314395.1 | 74.6% | 70.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1227
- ORF length:
- 1161
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgagacactc ttctggactc ctctcctcgt ggttctcctg gcagggctgg 121 gggacaccga ggcccagcag accacgctac acccacttgt gggccgtgtc tttgtgcaca 181 ccttggacca tgagacgttt ctgagccttc ctgagcatgt cgctgtccca cccgctgtcc 241 acatcaccta ccacgcccac ctccagggac acccagacct gccccggtgg ctccgctaca 301 cccagcgcag cccccaccac cctggcttcc tctacggctc tgccacccca gaagatcgtg 361 ggctccaggt cattgaggtc acagcctaca atcgggacag ctttgatacc actcggcaga 421 ggctggtgct ggagattggg gacccagaag gccccctgct gccataccaa gccgagttcc 481 tggtgcgcag ccacgatgcg gaggaggtgc tgccctcaac acctgccagc cgcttcctct 541 cagccttggg gggactctgg gagcccggag agcttcagct gctcaacgtc acctctgcct 601 tggaccgtgg gggccgtgtc ccccttccca ttgagggccg aaaagaaggg gtatacatta 661 aggtgggttc tgcctcacct ttttctactt gcctgaagat ggtggcatcc cccgatagcc 721 acgcccgctg tgcccagggc cagcctccac ttctgtcttg ctacgacacc ttggcacccc 781 acttccgcgt tgactggtgc aatgtgaccc tggtggataa gtcagtgccg gagcctgcag 841 atgaggtgcc caccccaggt gatgggatcc TGGAGCATGA CCCGTTCTTC TGCCCACCCA 901 CTGAGGCCCC AGACCGTGAC TTCTTGGTGG ATGCTCTGGT CACCCTCCTG GTGCCCCTGC 961 TGGTGGCCCT GCTTCTCACC TTGCTGCTGG CCTATGTCAT GTGCTGCCGG CGGGAGGGAA 1021 GGCTGAAGAG AGACCTGGCT ACCTCCGACA TCCAGATGGT CCACCACTGC ACCATCCACG 1081 GGAACACAGA GGAGCTGCGG CAGATGGCGG CCAGCCGCGA GGTGCCCCGG CCACTCTCCA 1141 CCCTGCCCAT GTTCAATGTG CACACAGGTG AGCGGCTGCC TCCCCGCGTG GACAGCGCCC 1201 AGGTGCCCCT CATTCTGGAC CAGCACTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 1261 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1321 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATCTGTTAT 1381 TACTGCCATC TCCATCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1441 aagatt