Construct: ORF TRCN0000478871
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012258.1_s317c1
- Derived from:
- ccsbBroadEn_12784
- DNA Barcode:
- ATGGCTGCTTTCCGGAGTGTTTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ABRAXAS1 (84142)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478871
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84142 | ABRAXAS1 | abraxas 1, BRCA1 A complex ... | NM_001345962.2 | 100% | 100% | |
| 2 | human | 84142 | ABRAXAS1 | abraxas 1, BRCA1 A complex ... | XM_005263280.5 | 83.3% | 83.3% | 1_180del |
| 3 | human | 84142 | ABRAXAS1 | abraxas 1, BRCA1 A complex ... | NM_139076.3 | 73.3% | 73.3% | 1_327del |
| 4 | human | 84142 | ABRAXAS1 | abraxas 1, BRCA1 A complex ... | XR_001741334.2 | 29.1% | 1_392del;743_878del;944_945ins484 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 966
- ORF length:
- 900
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac gtttagagag aggctgcttc acaaaaactt gcaggagcat ttttcaaacc 121 aagaccttgt ttttctgcta ttaacaccaa gtataataac agaaagctgc tctactcatc 181 gactggaaca ttccttatat aaacctcaaa aaggactttt tcacagggta cctttagtgg 241 ttgccaatct gggcatgtct gaacaactgg gttataaaac tgtatcaggt tcctgtatgt 301 ccactggttt tagccgagca gtacaaacac acagctctaa attttttgaa gaagatggat 361 ccttaaagga ggtacataag ataaatgaaa tgtatgcttc attacaagag gaattaaaga 421 gtatatgcaa aaaagtggaa gacagtgaac aagcagtaga taaactagta aaggatgtaa 481 acagattaaa acgagaaatt gagaaaagga gaggagcaca gattcaggca gcaagagaga 541 agaacatcca aaaagaccct caggagaaca tttttctttg tcaggcatta cggacctttt 601 ttccaaattc TGAATTTCTT CATTCATGTG TTATGTCTTT AAAAAATAGA CATGTTTCTA 661 AAAGTAGCTG TAACTACAAC CACCATCTCG ATGTAGTAGA CAATCTGACC TTAATGGTAG 721 AACACACTGA CATTCCTGAA GCTAGTCCAG CTAGTACACC ACAAATCATT AAGCATAAAG 781 CCTTAGACTT AGATGACAGA TGGCAATTCA AGAGATCTCG GTTGTTAGAT ACACAAGACA 841 AACGATCTAA AGCAGATACT GGTAGTAGTA ACCAAGATAA AGCATCCAAA ATGAGCAGCC 901 CAGAAACAGA TGAAGAAATT GAAAAGATGA AGGGTTTTGG TGAATATTCA CGGTCTCCTA 961 CATTTTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1021 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1081 CTTGGCTTTA TATATCTTGT GGAAAGGACG AATGGCTGCT TTCCGGAGTG TTTTAACGCG 1141 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt