Construct: ORF TRCN0000478893
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018182.1_s317c1
- Derived from:
- ccsbBroadEn_03616
- DNA Barcode:
- GGCCGATATCCAGTCTGGGTCGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DEPDC1 (55635)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478893
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55635 | DEPDC1 | DEP domain containing 1 | NM_017779.6 | 100% | 100% | |
| 2 | human | 55635 | DEPDC1 | DEP domain containing 1 | NM_001114120.3 | 64.9% | 64.8% | 911_1762del |
| 3 | mouse | 76131 | Depdc1a | DEP domain containing 1a | NM_001172093.1 | 87.2% | 85.2% | (many diffs) |
| 4 | mouse | 76131 | Depdc1a | DEP domain containing 1a | NM_001172092.1 | 57.2% | 55.8% | (many diffs) |
| 5 | mouse | 76131 | Depdc1a | DEP domain containing 1a | NM_029523.3 | 56.9% | 55.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1647
- ORF length:
- 1581
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gagtcagggt gtgcctcccg ggccttatcg ggccaccaag ctgtggaatg 121 aagttaccac atcttttcga gcaggaatgc ctctaagaaa acacagacaa cactttaaaa 181 aatatggcaa ttgtttcaca gcaggagaag cagtggattg gctttatgac ctattaagaa 241 ataatagcaa ttttggtcct gaagttacaa ggcaacagac tatccaactg ttgaggaaat 301 ttcttaagaa tcatgtaatt gaagatatca aagggaggtg gggatcagaa aatgttgatg 361 ataacaacca gctcttcaga tttcctgcaa cttcgccact taaaactcta ccacgaaggt 421 atccagaatt gagaaaaaac aacatagaga acttttccaa agataaagat agcattttta 481 aattacgaaa cttatctcgt agaactccta aaaggcatgg attacattta tctcaggaaa 541 atggcgagaa aataaagcat gaaataatca atgaagatca agaaaatgca attgataata 601 gagaactaag ccaggaagat gttgaagaag tttggagata tgttattctg atctacctgc 661 aaaccatttt aggtgtgcca tccctagaag aagtcataaa tccaaaacaa gtaattcccc 721 aatatataat gtacaacatg gccaatacaa gtaaacgtgg agtagttata ctacaaaaca 781 aatcagatga cctccctcac tgggtattat ctgccatgaa gtgcctagca aattggccaa 841 gaagcaatga tatgaataat ccaacttatg ttggatttga acgagatgta ttcagaacaa 901 tcgcagatta ttttctagat ctccctgaac ctctacttac ttttgaatat tacgaattat 961 ttgtaaacat tttgggcttg ctgcaacctc atttagagag ggttgccatc gatgctctac 1021 agttatgttg tttgttactt cccccaccaa atcgtagaaa gcttcaactt ttaatgcgta 1081 tgatttcccg aatgagtcaa aatgttgata tgcccaaact tcatgatgca atgggtacga 1141 ggtcactgat gatacatacc ttttctcgat gtgtgttatg ctgtgctgaa gaagtggatc 1201 ttgatgagct tcttgctgga agattagttt ctttcttaat ggatcatcat caggaaattc 1261 ttcaagtacc cTCTTACTTA CAGACTGCAG TGGAAAAACA TCTTGACTAC TTAAAAAAGG 1321 GACATATTGA AAATCCTGGA GATGGACTAT TTGCTCCTTT GCCAACTTAC TCATACTGTA 1381 AGCAGATTAG TGCTCAGGAG TTTGATGAGC AAAAAGTTTC TACCTCTCAA GCTGCAATTG 1441 CAGAACTTTT AGAAAATATT ATTAAAAACA GGAGTTTACC TCTAAAGGAG AAAAGAAAAA 1501 AACTAAAACA GTTTCAGAAG GAATATCCTT TGATATATCA GAAAAGATTT CCAACCACGG 1561 AGAGTGAAGC AGCACTTTTT GGTGACAAAC CTACAATCAA GCAACCAATG CTGATTTTAA 1621 GAAAACCAAA GTTCCGTAGT CTAAGATACC CAACTTTCTT GTACAAAGTG GTTGATATCG 1681 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1741 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGGCCGATA 1801 TCCAGTCTGG GTCGCGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1861 aagatt