Construct: ORF TRCN0000478915
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012952.1_s317c1
- Derived from:
- ccsbBroadEn_13019
- DNA Barcode:
- AAGTGCTATTCTTCTGGTCCATGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CASC4 (113201)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478915
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 113201 | CASC4 | cancer susceptibility 4 | NM_138423.4 | 96.7% | 94.3% | (many diffs) |
| 2 | human | 113201 | CASC4 | cancer susceptibility 4 | NM_177974.3 | 86.1% | 82.8% | (many diffs) |
| 3 | human | 113201 | CASC4 | cancer susceptibility 4 | XM_017021880.2 | 61.8% | 61.4% | (many diffs) |
| 4 | human | 113201 | CASC4 | cancer susceptibility 4 | NR_157849.2 | 23.2% | (many diffs) | |
| 5 | mouse | 319996 | Casc4 | cancer susceptibility candi... | NM_001205369.1 | 87.7% | 84.5% | (many diffs) |
| 6 | mouse | 319996 | Casc4 | cancer susceptibility candi... | NM_177054.5 | 81.2% | 78.4% | (many diffs) |
| 7 | mouse | 319996 | Casc4 | cancer susceptibility candi... | NM_001205370.1 | 77.5% | 73% | (many diffs) |
| 8 | mouse | 319996 | Casc4 | cancer susceptibility candi... | NM_199038.3 | 70.9% | 66.8% | (many diffs) |
| 9 | mouse | 319996 | Casc4 | cancer susceptibility candi... | NM_001205371.1 | 62.5% | 58% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1365
- ORF length:
- 1299
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gggtttcggg gccaaccggc gggctggccg cctgccctct ctcgtgctgg 121 tggtgctgct ggtggtgatc gtcgtcctcg ccttcaacta ctggagcatc tcctcccgcc 181 acgtcctgct tcaggaggag gtggccgagc tgcagggcca ggtccagcgc accgaagtgg 241 cccgcgggcg gctggaaaag cgcaattcgg acctcttgct gttggtggac acgcacaaga 301 aacagatcga ccagaaggag gccgactacg gccgcctcag cagccggctg caggccagag 361 agggcctcgg gaagagatgc gaggatgaca aggttaaact acagaacaac atatcgtatc 421 agatggcaga catacatcat ttaaaggagc aacttgctga gcttcgtcag gaatttcttc 481 gacaagaaga ccagcttcag gactatagga agaacaatac ttaccttgtg aagaggttag 541 aatatgaaag ttttcagtgt ggacagcaga tgaaggaatt gagagcacag catgaagaaa 601 atattaaaaa gttagcagac cagtttttag aggaacaaaa gcaagagacc caaaagattc 661 aatcaaatga tggaaaggaa ttggatataa acaatcaagt agtacctaaa aatattccaa 721 aagtagctga gaatgttgca gataagaatg aagaaccctc aagcaatcat attccacatg 781 ggaaagaaca aatcaaaaga ggtggtgatg cagggatgcc tggaatagaa gagaatgacc 841 tagcaaaagt tgatgatctt ccccctgctt taaggaagcc tcctatttca gtttctcaac 901 atgaaagtca tcaagcaatc tcccatcttc caactggaca acctctctcc ccaaatatgc 961 ctccagattc acacataaac cacaatggaa accccggtac ttcaaaacag aaTCCTTCCA 1021 GTCCTCTTCA GCGTTTAATT CCAGGCTCAA ACTTGGACAG TGAACCCAGA ATTCAAACAG 1081 ATATACTAAA GCAGGCTACC AAGGACAGAG TCAGTGATTT CCATAAATTG AAGCAAAGCC 1141 GATTCTTTGA TGAAAATGAA TCCCCTGTTG ATCCGCAGCA TGGCTCTAAA CTGGCGGATT 1201 ATAATGGGGA TGATGGTAAC GTAGGTGAGT ATGAGGCAGA CAAGCAGGCT GAGCTGGCTT 1261 ACAATGAGGA AGAAGATGGT GATGGTGGAG AGGAAGACGT CCAAGGTGAG CGTGGGCCTG 1321 GCCTCCATGC TATAACCATG AAACCCACCT CTAAGTTTTT TGGTTGCCCA ACTTTCTTGT 1381 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1441 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1501 GAAAGGACGA AAGTGCTATT CTTCTGGTCC ATGCACGCGT TAAGTCgaca atcaacctct 1561 ggattacaaa atttgtgaaa gatt