Construct: ORF TRCN0000478935
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017854.1_s317c1
- Derived from:
- ccsbBroadEn_07066
- DNA Barcode:
- CAGCACGGCCAGTTGTCAAACAGA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- THRA (7067)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478935
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7067 | THRA | thyroid hormone receptor alpha | NM_001190919.1 | 99.9% | 99.7% | 1470_1471insC |
| 2 | human | 7067 | THRA | thyroid hormone receptor alpha | NM_003250.6 | 99.9% | 99.7% | 1470_1471insC |
| 3 | human | 7067 | THRA | thyroid hormone receptor alpha | NM_001190918.2 | 91.9% | 91.8% | 1108_1109ins117;1353_1354insC |
| 4 | human | 7067 | THRA | thyroid hormone receptor alpha | NM_199334.5 | 81.8% | 77.4% | (many diffs) |
| 5 | mouse | 21833 | Thra | thyroid hormone receptor alpha | NM_001313983.1 | 95.5% | 96.9% | (many diffs) |
| 6 | mouse | 21833 | Thra | thyroid hormone receptor alpha | NM_178060.4 | 78.4% | 76.8% | (many diffs) |
| 7 | mouse | 21833 | Thra | thyroid hormone receptor alpha | XR_001779972.1 | 44.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1539
- ORF length:
- 1473
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga acagaagcca agcaaggtgg agtgtgggtc agacccagag gagaacagtg 121 ccaggtcacc agatggaaag cgaaaaagaa agaacggcca atgttccctg aaaaccagca 181 tgtcagggta tatccctagt tacctggaca aagacgagca gtgtgtcgtg tgtggggaca 241 aggcaactgg ttatcactac cgctgtatca cttgtgaggg ctgcaagggc ttctttcgcc 301 gcacaatcca gaagaacctc catcccacct attcctgcaa atatgacagc tgctgtgtca 361 ttgacaagat cacccgcaat cagtgccagc tgtgccgctt caagaagtgc atcgccgtgg 421 gcatggccat ggacttggtt ctagatgact cgaagcgggt ggccaagcgt aagctgattg 481 agcagaaccg ggagcggcgg cggaaggagg agatgatccg atcactgcag cagcgaccag 541 agcccactcc tgaagagtgg gatctgatcc acattgccac agaggcccat cgcagcacca 601 atgcccaggg cagccattgg aaacagaggc ggaaattcct gcccgatgac attggccagt 661 cacccattgt ctccatgccg gacggagaca aggtggacct ggaagccttc agcgagttta 721 ccaagatcat caccccggcc atcacccgtg tggtggactt tgccaaaaaa ctgcccatgt 781 tctccgagct gccttgcgaa gaccagatca tcctcctgaa ggggtgctgc atggagatca 841 tgtccctgcg ggcggctgtc cgctacgacc ctgagagcga caccctgacg ctgagtgggg 901 agatggctgt caagcgggag cagctcaaga atggcggcct gggcgtagtc tccgacgcca 961 tctttgaact gggcaagtca ctctctgcct ttaacctgga tgacacggaa gtggctctgc 1021 tgcaggctgt gctgctaatg tcaacagacc gctcgggcct gctgtgtgtg gacaagatcg 1081 agaagagtca ggaggcgtac ctgctggcgt tcgagcacta cgtcaaccac cgcaaacaca 1141 aCATTCCGCA CTTCTGGCCC AAGCTGCTGA TGAAGGAGAG AGAAGTGCAG AGTTCGATTC 1201 TGTACAAGGG GGCAGCGGCA GAAGGCCGGC CGGGCGGGTC ACTGGGCGTC CACCCGGAAG 1261 GACAGCAGCT TCTCGGAATG CATGTTGTTC AGGGTCCGCA GGTCCGGCAG CTTGAGCAGC 1321 AGCTTGGTGA AGCGGGAAGT CTCCAAGGGC CGGTTCTTCA GCACCAGAGC CCGAAGAGCC 1381 CGCAGCAGCG TCTCCTGGAG CTGCTCCACC GAAGCGGAAT TCTCCATGCC CGAGCGGTCT 1441 GTGGGGAAGA CGACAGCAGT GAGGCGGACT CCCCGAGCTC CTCTGAGGAG GAACCGGAGG 1501 TCTGCGAGGA CCTGGCAGGC AATGCAGCCT CTCCCCTGCC CAACTTTCTT GTACAAAGTG 1561 GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA 1621 ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC 1681 GACAGCACGG CCAGTTGTCA AACAGAACGC GTTAAGTCga caatcaacct ctggattaca 1741 aaatttgtga aagatt