Construct: ORF TRCN0000478938
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013997.1_s317c1
- Derived from:
- ccsbBroadEn_05076
- DNA Barcode:
- ATCTGGGATGTCCCTGGAATAAGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRELID2 (153768)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478938
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 153768 | PRELID2 | PRELI domain containing 2 | NM_138492.6 | 100% | 100% | |
| 2 | human | 153768 | PRELID2 | PRELI domain containing 2 | NM_205846.3 | 83.6% | 83.6% | 1_87del |
| 3 | human | 153768 | PRELID2 | PRELI domain containing 2 | NM_182960.4 | 78.3% | 78.3% | 1_87del;205_240del |
| 4 | human | 153768 | PRELID2 | PRELI domain containing 2 | XM_017009129.1 | 78.3% | 78.3% | 1_87del;205_240del |
| 5 | human | 153768 | PRELID2 | PRELI domain containing 2 | XM_017009130.1 | 78.3% | 78.3% | 1_87del;205_240del |
| 6 | human | 153768 | PRELID2 | PRELI domain containing 2 | XM_017009131.1 | 78.3% | 78.3% | 1_87del;205_240del |
| 7 | human | 153768 | PRELID2 | PRELI domain containing 2 | XM_017009128.1 | 72% | 67% | (many diffs) |
| 8 | human | 153768 | PRELID2 | PRELI domain containing 2 | XM_017009127.1 | 69.5% | 64.8% | (many diffs) |
| 9 | human | 153768 | PRELID2 | PRELI domain containing 2 | XM_017009135.1 | 57.6% | 53.6% | 1_87del;367_368ins106;393_394ins32 |
| 10 | human | 153768 | PRELID2 | PRELI domain containing 2 | XM_017009132.1 | 53.9% | 50.2% | (many diffs) |
| 11 | human | 153768 | PRELID2 | PRELI domain containing 2 | XM_017009133.1 | 53.9% | 50.2% | (many diffs) |
| 12 | human | 153768 | PRELID2 | PRELI domain containing 2 | XM_017009134.1 | 53.9% | 50.2% | (many diffs) |
| 13 | human | 153768 | PRELID2 | PRELI domain containing 2 | XR_001742021.1 | 37.9% | (many diffs) | |
| 14 | human | 153768 | PRELID2 | PRELI domain containing 2 | XR_001742019.1 | 36.7% | (many diffs) | |
| 15 | human | 153768 | PRELID2 | PRELI domain containing 2 | XR_001742024.2 | 32.5% | (many diffs) | |
| 16 | human | 153768 | PRELID2 | PRELI domain containing 2 | XR_001742022.2 | 31.5% | (many diffs) | |
| 17 | human | 153768 | PRELID2 | PRELI domain containing 2 | XR_001742025.1 | 29.2% | (many diffs) | |
| 18 | mouse | 77619 | Prelid2 | PRELI domain containing 2 | NM_029942.1 | 73.8% | 74.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 510
- ORF length:
- 444
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga taaaaatgtc atctcagtaa aaatcatgga ggaaaaaaga gatgaatcaa 121 caggggTCAT CTACAGAAAG AGGATTGCAA TCTGTCAGAA CGTGGTTCCA GAAATTTTAA 181 GGAAGGTGAG CATTTTGAAA GTACCTAATA TCCAATTAGA AGAGGAGTCA TGGCTCAATC 241 CTCGGGAAAG AAACATGGCC ATACGGAGTC ACTGCCTTAC GTGGACACAG TATGCATCCA 301 TGAAGGAAGA GTCTGTCTTC CGGGAAAGTA TGGAAAACCC AAATTGGACA GAGTTCATTC 361 AAAGAGGCAG GATTTCAATC ACAGGGGTTG GATTTCTCAA CTGTGTTTTA GAAACTTTTG 421 CCAGCACATT CTTACGACAG GGAGCCCAGA AGGGAATTAG AATCATGGAG ATGCTGCTAA 481 AGGAACAGTG TGGTGCCCCC TTAGCTGAAT ACCCAACTTT CTTGTACAAA GTGGTTGATA 541 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 601 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAATCTG 661 GGATGTCCCT GGAATAAGAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 721 tgaaagatt