Construct: ORF TRCN0000478940
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010729.2_s317c1
- Derived from:
- ccsbBroadEn_09330
- DNA Barcode:
- ATGAGACCATCGGCACTAGAAAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MTDH (92140)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478940
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 92140 | MTDH | metadherin | NM_178812.4 | 99.8% | 99.6% | 232G>T;949A>G |
| 2 | human | 92140 | MTDH | metadherin | NM_001363136.1 | 96.1% | 95.8% | 232G>T;744_745ins66;883A>G |
| 3 | human | 92140 | MTDH | metadherin | NM_001363137.1 | 94.9% | 94.7% | 232G>T;949A>G;1047_1136del |
| 4 | human | 92140 | MTDH | metadherin | NM_001363138.1 | 94.2% | 93.8% | 232G>T;949A>G;1048_1049ins99 |
| 5 | human | 92140 | MTDH | metadherin | XM_011517370.3 | 91.8% | 91.5% | 232G>T;949A>G;1377_1378ins141 |
| 6 | human | 92140 | MTDH | metadherin | XM_011517368.2 | 91.3% | 91.1% | (many diffs) |
| 7 | human | 92140 | MTDH | metadherin | NM_001363139.1 | 90.4% | 90% | (many diffs) |
| 8 | human | 92140 | MTDH | metadherin | XM_011517369.3 | 87.3% | 87% | (many diffs) |
| 9 | human | 92140 | MTDH | metadherin | XM_017013968.2 | 86.1% | 85.7% | (many diffs) |
| 10 | mouse | 67154 | Mtdh | metadherin | NM_026002.4 | 90.2% | 90.7% | (many diffs) |
| 11 | mouse | 67154 | Mtdh | metadherin | XM_006520148.1 | 85.8% | 86.2% | (many diffs) |
| 12 | mouse | 67154 | Mtdh | metadherin | XM_006520150.1 | 84.7% | 84.8% | (many diffs) |
| 13 | mouse | 67154 | Mtdh | metadherin | XM_017316729.1 | 80.9% | 80.2% | (many diffs) |
| 14 | mouse | 67154 | Mtdh | metadherin | XM_017316728.1 | 80.4% | 80.7% | (many diffs) |
| 15 | mouse | 67154 | Mtdh | metadherin | XM_006520151.1 | 76.9% | 76.3% | (many diffs) |
| 16 | mouse | 67154 | Mtdh | metadherin | XM_006520149.1 | 76.4% | 76.7% | (many diffs) |
| 17 | mouse | 67154 | Mtdh | metadherin | XM_006520152.1 | 75.3% | 74.3% | (many diffs) |
| 18 | mouse | 67154 | Mtdh | metadherin | XM_017316730.1 | 74.8% | 74.9% | (many diffs) |
| 19 | mouse | 67154 | Mtdh | metadherin | XM_017316731.1 | 67.5% | 66.8% | (many diffs) |
| 20 | mouse | 67154 | Mtdh | metadherin | XM_006520153.3 | 49.8% | 48.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1812
- ORF length:
- 1746
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgcacggagc tggcaggacg agctggccca gcaggccgag gagggctcgg 121 cccggctgcg ggaaatgctc tcggtcggcc taggctttct gcgcaccgag ctgggcctcg 181 acctggggct ggagccgaaa cggtaccccg gctgggtgat cctggtgggc actggcgcgc 241 tcgggctgct gctgctgttt ctgctgggct acggctgggc cgcggcttgc gccggctccc 301 gcaaaaagcg gaggagcccg ccccgcaagc gggaggaggc ggcggccgtg ccggccgcgg 361 cccccgacga cctggccttg ctgaagaatc tccggagcga ggaacagaag aagaagaacc 421 ggaagaaact gtccgagaag cccaaaccaa atgggcggac tgttgaagtg gctgagggtg 481 aagctgttcg aacacctcaa agtgtaacag caaagcagcc accagagatt gacaagaaaa 541 atgaaaagtc aaagaaaaat aagaagaaat caaagtcaga tgctaaagca gtgcaaaaca 601 gttcacgcca tgatggaaag gaagttgatg aaggagcctg ggaaactaaa attagtcaca 661 gagagaaacg acagcagcgt aaacgtgata aggtgctgac tgattctggt tcattggatt 721 caactatccc tgggatagaa aataccatca cagttaccac cgagcaactt acaaccgcat 781 catttcctgt tggttccaag aagaataaag gtgattctca tctaaatgtt caagttagca 841 actttaaatc tggaaaagga gattctacac ttcaggtttc ttcaggattg aatgaaaacc 901 tcactgtcaa tggaggaggc tggaatgaaa agtctgtaaa actctcctca cagatcagtg 961 caggtgagga gaagtggaac tccgtttcac ctgcttctgc aggaaagagg aaagctgagc 1021 catctgcctg gagtcaagac actggagatg ctaatacaaa tggaaaagac tggggaagga 1081 gttggagtga ccgttcaata ttttctggca ttgggtctac tgctgagcca gtttctcagt 1141 ctaccacttc tgattatcag tgggatgtta gccgtaatca accctatatc gatgatgaat 1201 ggtctgggtt aaatggtctg tcttctgctg atcccaactc tgattggaat gcaccagcag 1261 aagagtgggg caattgggta gacgaagaaa gagcttcact tctaaagtcc caggaaccaa 1321 ttcctgatga tcaaaaggtc tcagatgatg ataaagaaaa gggagaggga gctcttccaa 1381 ctgggaaatc caaaaagaaa aaaaaGAAAA AGAAGAAGCA AGGTGAAGAT AACTCTACTG 1441 CACAGGACAC AGAAGAATTA GAAAAAGAGA TTAGAGAAGA CCTTCCAGTG AATACCTCTA 1501 AAACCCGTCC AAAACAGGAA AAAGCTTTTT CCTTGAAGAC CATAAGCACT AGTGATCCAG 1561 CCGAAGTACT CGTCAAAAAT AGCCAGCCTA TCAAGACTCT TCCACCTGCT ACTTCTACCG 1621 AGCCATCTGT AATCTTATCA AAAAGTGATT CTGACAAGAG CTCTTCCCAA GTGCCGCCAA 1681 TACTACAAGA GACAGATAAA TCCAAGTCAA ATACCAAGCA AAATAGTGTG CCTCCTTCAC 1741 AGACCAAGTC TGAAACTAGC TGGGAATCTC CCAAACAAAT AAAAAAGAAG AAAAAAGCCA 1801 GACGAGAAAC GTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1861 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1921 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAATG AGACCATCGG CACTAGAAAA 1981 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t