Transcript: Mouse XM_017316730.1

PREDICTED: Mus musculus metadherin (Mtdh), transcript variant X7, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Mtdh (67154)
Length:
4338
CDS:
100..1563

Additional Resources:

NCBI RefSeq record:
XM_017316730.1
NBCI Gene record:
Mtdh (67154)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_017316730.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000125818 AGCCATCTATTACCTTATCAA pLKO.1 1370 CDS 100% 5.625 7.875 N Mtdh n/a
2 TRCN0000312352 AGCCATCTATTACCTTATCAA pLKO_005 1370 CDS 100% 5.625 7.875 N Mtdh n/a
3 TRCN0000313386 GAACGAGAATGATCGTAATTT pLKO_005 1794 3UTR 100% 15.000 10.500 N Mtdh n/a
4 TRCN0000151559 GCCTTATTAATGGACAGCTTT pLKO.1 3026 3UTR 100% 4.950 3.465 N MTDH n/a
5 TRCN0000125817 GCAGGTTTCTTCAAGGCTGAA pLKO.1 726 CDS 100% 4.050 2.835 N Mtdh n/a
6 TRCN0000312291 GCAGGTTTCTTCAAGGCTGAA pLKO_005 726 CDS 100% 4.050 2.835 N Mtdh n/a
7 TRCN0000125815 CCCAAGTCAAATGCTAAGCAA pLKO.1 1450 CDS 100% 3.000 2.100 N Mtdh n/a
8 TRCN0000312360 CCCAAGTCAAATGCTAAGCAA pLKO_005 1450 CDS 100% 3.000 2.100 N Mtdh n/a
9 TRCN0000125814 GCTGAGGAATAATGAATGGAT pLKO.1 1881 3UTR 100% 3.000 2.100 N Mtdh n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017316730.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09330 pDONR223 100% 74.8% 74.9% None (many diffs) n/a
2 ccsbBroad304_09330 pLX_304 0% 74.8% 74.9% V5 (many diffs) n/a
3 TRCN0000478940 ATGAGACCATCGGCACTAGAAAAC pLX_317 23.6% 74.8% 74.9% V5 (many diffs) n/a
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