Construct: ORF TRCN0000478956
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015191.1_s317c1
- Derived from:
- ccsbBroadEn_07132
- DNA Barcode:
- GGGCGAGAATCGAAAAATCGCACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VSNL1 (7447)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478956
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7447 | VSNL1 | visinin like 1 | NM_001366803.1 | 99.8% | 99.4% | 16A>T |
| 2 | human | 7447 | VSNL1 | visinin like 1 | NM_001366806.1 | 99.8% | 99.4% | 16A>T |
| 3 | human | 7447 | VSNL1 | visinin like 1 | NM_003385.5 | 99.8% | 99.4% | 16A>T |
| 4 | human | 51440 | HPCAL4 | hippocalcin like 4 | NM_001282396.1 | 80.6% | 89% | (many diffs) |
| 5 | human | 51440 | HPCAL4 | hippocalcin like 4 | NM_016257.4 | 80.6% | 89% | (many diffs) |
| 6 | human | 51440 | HPCAL4 | hippocalcin like 4 | XM_017001447.1 | 80.6% | 89% | (many diffs) |
| 7 | human | 7447 | VSNL1 | visinin like 1 | NM_001366804.1 | 70.6% | 54% | (many diffs) |
| 8 | human | 7447 | VSNL1 | visinin like 1 | NM_001366805.1 | 62.1% | 61.7% | 16A>T;160_161ins216 |
| 9 | mouse | 26950 | Vsnl1 | visinin-like 1 | NM_012038.4 | 91.6% | 99.4% | (many diffs) |
| 10 | mouse | 170638 | Hpcal4 | hippocalcin-like 4 | NM_174998.4 | 81.1% | 89% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 639
- ORF length:
- 573
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gaagcagaat tgcaaactgg cccctgaagt gatggaggac ctggtgaaga 121 gcacagagtt taatgagcat gaactcaagc agtggtacaa aggatttctc aaggactgtc 181 caagtgggag gctaaatctc gaggaatttc agcagctcta tgtgaagttc tttccttatg 241 gagacgcctc caagtttgcc cagcatgcct tccgaacctt cgacaagaat gggGACGGCA 301 CCATTGACTT CCGAGAGTTC ATCTGCGCTC TGTCCATCAC CTCCAGGGGC AGCTTTGAGC 361 AGAAGCTGAA CTGGGCCTTC AATATGTATG ACCTGGATGG TGATGGCAAG ATCACCCGAG 421 TGGAGATGCT GGAGATCATC GAGGCTATCT ACAAAATGGT AGGCACTGTG ATCATGATGA 481 AAATGAATGA GGATGGCCTG ACGCCTGAGC AGCGAGTAGA CAAGATTTTC AGCAAGATGG 541 ATAAGAACAA AGATGACCAG ATTACACTGG ATGAATTCAA AGAAGCTGCA AAGAGCGACC 601 CTTCCATTGT ATTACTTCTG CAGTGCGACA TCCAGAAATG CCCAACTTTC TTGTACAAAG 661 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 721 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 781 ACGAGGGCGA GAATCGAAAA ATCGCACTAC GCGTTAAGTC gacaatcaac ctctggatta 841 caaaatttgt gaaagatt