Construct: ORF TRCN0000478960
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007352.3_s317c1
- Derived from:
- ccsbBroadEn_02674
- DNA Barcode:
- AATTAGCCCCCACTGATCTCTTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STK38 (11329)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478960
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11329 | STK38 | serine/threonine kinase 38 | NM_001305102.2 | 100% | 100% | |
| 2 | human | 11329 | STK38 | serine/threonine kinase 38 | NM_007271.4 | 100% | 100% | |
| 3 | human | 11329 | STK38 | serine/threonine kinase 38 | XM_006714988.4 | 100% | 100% | |
| 4 | human | 11329 | STK38 | serine/threonine kinase 38 | XM_006714989.4 | 100% | 100% | |
| 5 | human | 11329 | STK38 | serine/threonine kinase 38 | XM_017010226.1 | 93% | 90.8% | (many diffs) |
| 6 | human | 11329 | STK38 | serine/threonine kinase 38 | XR_001743146.1 | 35.6% | 1_292del;1126_1127ins118;1570_3467del | |
| 7 | mouse | 106504 | Stk38 | serine/threonine kinase 38 | NM_134115.2 | 92.8% | 98.7% | (many diffs) |
| 8 | mouse | 106504 | Stk38 | serine/threonine kinase 38 | XM_011246232.2 | 82.2% | 87.7% | (many diffs) |
| 9 | mouse | 106504 | Stk38 | serine/threonine kinase 38 | XM_011246233.2 | 82.2% | 87.7% | (many diffs) |
| 10 | mouse | 106504 | Stk38 | serine/threonine kinase 38 | XM_006523386.2 | 78.7% | 83.7% | (many diffs) |
| 11 | mouse | 106504 | Stk38 | serine/threonine kinase 38 | XM_006523385.2 | 78.6% | 83.6% | (many diffs) |
| 12 | mouse | 106504 | Stk38 | serine/threonine kinase 38 | XM_017317194.1 | 57.9% | 61.5% | (many diffs) |
| 13 | mouse | 106504 | Stk38 | serine/threonine kinase 38 | XM_017317192.1 | 44.3% | 41.6% | (many diffs) |
| 14 | mouse | 106504 | Stk38 | serine/threonine kinase 38 | XM_017317193.1 | 34.4% | 32.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1461
- ORF length:
- 1395
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc aatgacaggc tcaacacctt gctcatccat gagtaaccac acaaaggaaa 121 gggtgacaat gaccaaagtg acactggaga atttttatag caaccttatc gctcaacatg 181 aagaacgaga aatgagacaa aagaagttag aaaaggtgat ggaagaagaa ggcctaaaag 241 atgaggagaa acgactccgg agatcagcac atgctcggaa ggaaacagag tttcttcgtt 301 tgaagagaac aagacttgga ttggaagatt ttgagtcctt aaaagtaata ggcagaggag 361 catttggtga ggtacggctt gttcagaaga aagatacggg acatgtgtat gcaatgaaaa 421 tactccgtaa agcagatatg cttgaaaaag agcaggttgg ccacattcgt gcggagcgtg 481 acattctagt ggaggcagac agtttgtggg ttgtgaaaat gttctatagt tttcaggata 541 agctaaacct ctacctaatc atggagttcc tgcctggagg ggacatgatg accttgttga 601 tgaaaaaaga cactctgaca gaagaggaga ctcagtttta tatagcagaa acagtattag 661 ccatagactc tattcaccaa cttggattca tccacagaga catcaaacca gacaaccttc 721 ttttggacag caagggccat gtgaaacttt ctgactttgg tctttgcaca ggactgaaaa 781 aagcacatag gacagaattt tataggaatc tgaaccacag cctccccagt gatttcactt 841 tccagaacat gaattccaaa aggaaagcag aaacctggaa aagaaataga cgtcagctag 901 ccttctccac agtaggcact cctgactaca ttgctcctga ggtgttcatg cagaccgggt 961 acaacaagct ctgtgattgg tggtcgcttg gggtgatcat gtatgagatg ctcatcggct 1021 acccaccttt ctgttctgag acccctcaag agacatataa gaaggtgatg aactGGAAAG 1081 AAACTTTGAC TTTTCCTCCA GAAGTTCCCA TCTCTGAGAA AGCCAAGGAT CTAATTTTGA 1141 GGTTCTGCTG TGAATGGGAA CATAGAATTG GAGCTCCTGG AGTTGAGGAA ATAAAAAGTA 1201 ACTCTTTTTT TGAAGGCGTT GACTGGGAAC ATATCAGAGA GAGACCTGCT GCAATATCTA 1261 TTGAAATCAA AAGCATTGAT GATACCTCAA ACTTCGATGA GTTTCCAGAA TCTGATATTC 1321 TTAAGCCAAC AGTGGCCACA AGTAATCATC CTGAGACTGA CTACAAGAAC AAAGACTGGG 1381 TCTTCATCAA TTACACGTAC AAGCGCTTTG AGGGCCTGAC TGCAAGGGGG GCAATACCTT 1441 CCTACATGAA AGCAGCAAAA TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1501 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1561 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAAATT AGCCCCCACT 1621 GATCTCTTTG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt