Construct: ORF TRCN0000479035
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005332.2_s317c1
- Derived from:
- ccsbBroadEn_09507
- DNA Barcode:
- CTTGATCTACGCTTGTCTGCTGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PODN (127435)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479035
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 127435 | PODN | podocan | XM_017000294.2 | 95.2% | 94.4% | (many diffs) |
| 2 | human | 127435 | PODN | podocan | NM_001199080.4 | 92.5% | 92.5% | (many diffs) |
| 3 | human | 127435 | PODN | podocan | NM_001199081.3 | 92.5% | 92.5% | (many diffs) |
| 4 | human | 127435 | PODN | podocan | NM_153703.5 | 92.5% | 92.5% | (many diffs) |
| 5 | human | 127435 | PODN | podocan | XM_005270457.3 | 92.5% | 92.5% | (many diffs) |
| 6 | human | 127435 | PODN | podocan | XR_001736973.2 | 64.6% | (many diffs) | |
| 7 | human | 127435 | PODN | podocan | XM_011540677.2 | 60.7% | 60.6% | 0_1ins777;624G>A;782T>C |
| 8 | mouse | 242608 | Podn | podocan | NM_001285956.1 | 80.1% | 84.7% | (many diffs) |
| 9 | mouse | 242608 | Podn | podocan | NM_001285958.1 | 80.1% | 84.7% | (many diffs) |
| 10 | mouse | 242608 | Podn | podocan | NM_172874.3 | 80.1% | 84.7% | (many diffs) |
| 11 | mouse | 242608 | Podn | podocan | XM_006503092.4 | 80.1% | 84.7% | (many diffs) |
| 12 | mouse | 242608 | Podn | podocan | XM_011240532.1 | 52.9% | 56.4% | (many diffs) |
| 13 | mouse | 242608 | Podn | podocan | XM_011240534.1 | 52.9% | 56.4% | (many diffs) |
| 14 | mouse | 242608 | Podn | podocan | XM_030253568.1 | 52.9% | 56.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2049
- ORF length:
- 1983
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga aggagcccga gcccgcggag cgcagctgag actgggggag cgcgttcggc 121 ctgtggggcg ccgctcggcg ccggggcgca gcaggttcca tcagccctgg cgcccaggcg 181 catctgactc ggcaccccct gcaggcacca tggcccagag ccgggtgctg ctgctcctgc 241 tgctgctgcc gccacagctg cacctgggac ctgtgcttgc cgtgagggcc ccaggatttg 301 gccgaagtgg cggccacagc ctgagccccg aagagaacga atttgcggag gaggagccgg 361 tgctggtact gagccctgag gagcccgggc ctggcccagc cgcggtcagc tgcccccgag 421 actgtgcctg ttcccaggag ggcgtcgtgg actgtggcgg tattgacctg cgtgagttcc 481 cgggggacct gcctgagcac accaaccacc tatctctgca gaacaaccag ctggaaaaga 541 tctaccctga ggagctctcc cggctgcacc ggctggagac actgaacctg caaaacaacc 601 gcctgacttc ccgagggctc ccagagaagg cgtttgagca tctgaccaac ctcaattacc 661 tgtacttggc caataacaag ctgaccttgg caccccgctt cctgccaaac gccctgatca 721 gtgtggactt tgctgccaac tatctcacca agatctatgg gctcaccttt ggccagaagc 781 caaacttgag gtctgtgtac ctgcacaaca acaagctggc agacgccggg ctgccggaca 841 acatgttcaa cggctccagc aacgtcgagg tcctcatcct gtccagcaac ttcctgcgcc 901 acgtgcccaa gcacctgccg cctgccctgt acaagctgca cctcaagaac aacaagctgg 961 agaagatccc cccgggggcc ttcagcgagc tgagcagcct gcgcgagcta tacctgcaga 1021 acaactacct gactgacgag ggcctggaca acgagacctt ctggaagctc tccagcctgg 1081 agtacctgga tctgtccagc aacaacctgt ctcgggtccc agctgggctg ccgcgcagcc 1141 tggtgctgct gcacttggag aagaacgcca tccggagcgt ggacgcgaat gtgctgaccc 1201 ccatccgcag cctggagtac ctgctgctgc acagcaacca gctgcgggag cagggcatcc 1261 acccactggc cttccagggc ctcaagcggt tgcacacggt gcacctgtac aacaacgcgc 1321 tggagcgcgt gcccagtggc ctgcctcgcc gcgtgcgcac cctcatgatc ctgcacaacc 1381 agatcacagg cattggccgc gaagactttg ccaccaccta cttcctggag gagctcaacc 1441 tcagctacaa ccgcatcacc agcccacagg tgcaccgcga cgccttccgc aagctgcgcc 1501 tgctgcgctc gctggacctg tcgggcaacc ggctgcacac gctgccacct gggctgcctc 1561 gaaatgtcca tgtgctgaag gtcaagcgca atgagctggc tgccttggca cgaggggcgc 1621 tggcgggcat ggctcagctg cgtgagctgt acctcaccag caaCCGACTG CGCAGCCGAG 1681 CCCTGGGCCC CCGTGCCTGG GTGGACCTCG CCCATCTGCA GCTGCTGGAC ATCGCCGGGA 1741 ATCAGCTCAC AGAGATCCCC GAGGGGCTCC CCGAGTCACT TGAGTACCTG TACCTGCAGA 1801 ACAACAAGAT TAGTGCGGTG CCCGCCAATG CCTTCGACTC CACGCCCAAC CTCAAGGGGA 1861 TCTTTCTCAG GTTTAACAAG CTGGCTGTGG GCTCCGTGGT GGACAGTGCC TTCCGGAGGC 1921 TGAAGCACCT GCAGGTCTTG GACATTGAAG GCAACTTAGA GTTTGGTGAC ATTTCCAAGG 1981 ACCGTGGCCG CTTGGGGAAG GAAAAGGAGG AGGAGGAAGA GGAGGAGGAG GAGGAAGAGG 2041 AAACAAGATA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 2101 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 2161 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACTTGAT CTACGCTTGT CTGCTGAAAC 2221 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt