Construct: ORF TRCN0000479058
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001472.1_s317c1
- Derived from:
- ccsbBroadEn_04450
- DNA Barcode:
- GGGCAGGTCATACCACCGTCCTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNRF1 (84937)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479058
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84937 | ZNRF1 | zinc and ring finger 1 | NM_032268.5 | 100% | 100% | |
| 2 | human | 84937 | ZNRF1 | zinc and ring finger 1 | XM_017023793.1 | 100% | 100% | |
| 3 | human | 84937 | ZNRF1 | zinc and ring finger 1 | XM_017023794.1 | 72.3% | 64.6% | (many diffs) |
| 4 | human | 84937 | ZNRF1 | zinc and ring finger 1 | XM_011523392.1 | 62.4% | 62.5% | 426_426delAins256 |
| 5 | mouse | 170737 | Znrf1 | zinc and ring finger 1 | NM_001168621.1 | 95% | 99.1% | (many diffs) |
| 6 | mouse | 170737 | Znrf1 | zinc and ring finger 1 | NM_133206.3 | 95% | 99.1% | (many diffs) |
| 7 | mouse | 170737 | Znrf1 | zinc and ring finger 1 | XM_006530731.2 | 95% | 99.1% | (many diffs) |
| 8 | mouse | 170737 | Znrf1 | zinc and ring finger 1 | NM_001168622.1 | 81.9% | 85% | (many diffs) |
| 9 | mouse | 170737 | Znrf1 | zinc and ring finger 1 | XM_011248322.2 | 66.3% | 63.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 747
- ORF length:
- 681
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gggcaagcag agcacggcgg cccgctcccg gggccccttc ccgggggtct 121 ccaccgatga cagcgccgtg ccgccgccgg gaggggcgcc ccatttcggg cactaccgga 181 cgggcggcgg ggccatgggg ctgcgcagcc gctcggtcag ctcggtggca ggcatgggca 241 tggaccccag cacggccggg ggggtgccct ttggcctcta cacccccgcc tcccggggca 301 ccggcgactc cgagagggcg cccggcggcg gagggtctgc gtccgactcc accTATGCCC 361 ATGGCAATGG TTACCAGGAG ACGGGCGGCG GTCACCATAG AGACGGGATG CTGTACCTGG 421 GCTCCCGAGC CTCGCTGGCG GATGCTCTAC CTCTGCACAT CGCACCCAGG TGGTTCAGCT 481 CGCATAGTGG TTTCAAGTGC CCCATTTGCT CCAAGTCTGT GGCTTCTGAC GAGATGGAAA 541 TGCACTTTAT AATGTGTTTG AGCAAACCTC GCCTCTCCTA CAACGATGAT GTGCTGACTA 601 AAGACGCGGG TGAGTGTGTG ATCTGCCTGG AGGAGCTGCT GCAGGGGGAC ACGATAGCCA 661 GGCTGCCCTG CCTGTGCATC TATCACAAAA GCTGCATAGA CTCGTGGTTT GAAGTGAACA 721 GATCTTGTCC GGAACACCCT GCGGACTGCC CTACTTTCTT GTACAAAGTG GTTGATATCG 781 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 841 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGGGCAGGT 901 CATACCACCG TCCTCAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 961 aagatt