Construct: ORF TRCN0000479066
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006753.2_s317c1
- Derived from:
- ccsbBroadEn_01255
- DNA Barcode:
- GCCTTGTTTCACATTTAGACTTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPM1B (5495)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479066
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5495 | PPM1B | protein phosphatase, Mg2+/M... | NM_177969.3 | 100% | 100% | |
| 2 | human | 5495 | PPM1B | protein phosphatase, Mg2+/M... | NM_002706.6 | 40% | 40% | 1_861del |
| 3 | human | 5495 | PPM1B | protein phosphatase, Mg2+/M... | XM_017004395.2 | 39.4% | 39.4% | 1_861del;963_986del |
| 4 | human | 5495 | PPM1B | protein phosphatase, Mg2+/M... | XM_011532936.3 | 26.8% | 21.2% | (many diffs) |
| 5 | human | 5495 | PPM1B | protein phosphatase, Mg2+/M... | XM_017004396.2 | 26.4% | 20.9% | (many diffs) |
| 6 | human | 5495 | PPM1B | protein phosphatase, Mg2+/M... | NM_177968.4 | 20% | 19.2% | (many diffs) |
| 7 | human | 5495 | PPM1B | protein phosphatase, Mg2+/M... | XM_017004397.2 | 19.7% | 18.8% | (many diffs) |
| 8 | human | 5495 | PPM1B | protein phosphatase, Mg2+/M... | NM_001033557.3 | 19.4% | 18.9% | (many diffs) |
| 9 | mouse | 19043 | Ppm1b | protein phosphatase 1B, mag... | NM_001159496.1 | 35.9% | 34.6% | (many diffs) |
| 10 | mouse | 19043 | Ppm1b | protein phosphatase 1B, mag... | XM_006523860.3 | 35.9% | 34.6% | (many diffs) |
| 11 | mouse | 19043 | Ppm1b | protein phosphatase 1B, mag... | XM_011246321.2 | 35.9% | 34.6% | (many diffs) |
| 12 | mouse | 19043 | Ppm1b | protein phosphatase 1B, mag... | XM_011246322.2 | 35.9% | 34.6% | (many diffs) |
| 13 | mouse | 19043 | Ppm1b | protein phosphatase 1B, mag... | NM_001159498.1 | 19.1% | 17.5% | (many diffs) |
| 14 | mouse | 19043 | Ppm1b | protein phosphatase 1B, mag... | XM_006523861.3 | 19.1% | 17.5% | (many diffs) |
| 15 | mouse | 19043 | Ppm1b | protein phosphatase 1B, mag... | XM_011246323.1 | 19.1% | 17.5% | (many diffs) |
| 16 | mouse | 19043 | Ppm1b | protein phosphatase 1B, mag... | NM_011151.2 | 19% | 18.1% | (many diffs) |
| 17 | mouse | 19043 | Ppm1b | protein phosphatase 1B, mag... | NM_001159497.1 | 18.9% | 17.5% | (many diffs) |
| 18 | mouse | 19043 | Ppm1b | protein phosphatase 1B, mag... | XM_006523862.3 | 18.9% | 17.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 642
- ORF length:
- 576
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag tattgtacta gtttgctttt caaatgctcc caaggtctca gatgaagcgg 121 tgaaaaaaga ttcagagttg gataagcact tggaatcacg ggttgaagag attatggaga 181 agtctggcga ggaaggaatg cctgatcttg cccatgtcat gcgcatcttg tctgcagaaa 241 atatcccaaa tttgccTCCT GGGGGAGGTC TTGCTGGCAA GCGTAATGTT ATTGAAGCTG 301 TTTATAGTAG ACTGAATCCA CATAGAGAAA GTGATGGGGC CTCCGATGAA GCAGAGGAAA 361 GTGGATCACA GGGAAAATTG GTGGAAGCTC TCAGGCAAAT GAGAATTAAT CATAGGGGAA 421 ACTACCGACA ACTTCTGGAG GAGATGCTGA CTAGTTACAG GCTAGCTAAA GTAGAGGGAG 481 AAGAAAGCCC TGCTGAACCA GCTGCCACAG CTACTTCTTC GAACAGTGAT GCTGGAAACC 541 CAGTGACAAT GCAGGAAAGC CATACTGAAT CAGAAAGTGG TCTTGCTGAA TTAGACAGCT 601 CTAATGAAGA TGCAGGGACA AAGATGAGTG GTGAAAAAAT ATGCCCAACT TTCTTGTACA 661 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 721 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 781 AGGACGAGCC TTGTTTCACA TTTAGACTTA TACGCGTTAA GTCgacaatc aacctctgga 841 ttacaaaatt tgtgaaagat t