Construct: ORF TRCN0000479067
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005735.1_s317c1
- Derived from:
- ccsbBroadEn_16137
- DNA Barcode:
- CGACCCCTCCCCAGTTATGTTCCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- FRMD3 (257019)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479067
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 257019 | FRMD3 | FERM domain containing 3 | NM_001244962.2 | 99.8% | 34.7% | 217_218insC |
| 2 | human | 257019 | FRMD3 | FERM domain containing 3 | XM_017014591.2 | 83.7% | 29.1% | (many diffs) |
| 3 | human | 257019 | FRMD3 | FERM domain containing 3 | NM_001244961.1 | 52.7% | 8.6% | (many diffs) |
| 4 | human | 257019 | FRMD3 | FERM domain containing 3 | XM_017014589.1 | 47.5% | 7.7% | (many diffs) |
| 5 | human | 257019 | FRMD3 | FERM domain containing 3 | XM_024447487.1 | 38.3% | 6.2% | (many diffs) |
| 6 | human | 257019 | FRMD3 | FERM domain containing 3 | XM_024447488.1 | 38.3% | 6.2% | (many diffs) |
| 7 | human | 257019 | FRMD3 | FERM domain containing 3 | XM_024447489.1 | 38.3% | 6.2% | (many diffs) |
| 8 | human | 257019 | FRMD3 | FERM domain containing 3 | NM_001244960.1 | 34.8% | 5.6% | (many diffs) |
| 9 | human | 257019 | FRMD3 | FERM domain containing 3 | NM_001244959.2 | 34.6% | 5.6% | (many diffs) |
| 10 | human | 257019 | FRMD3 | FERM domain containing 3 | XM_017014588.1 | 34.6% | 5.6% | (many diffs) |
| 11 | human | 257019 | FRMD3 | FERM domain containing 3 | NM_174938.6 | 32.2% | 5.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 309
- ORF length:
- 243
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca ggccgaggag accaagggga ttggaataac cagtatggca aaatgcttgg 121 tcaagatcca gactcgacga agcctccaat tgcacatggt gaaccactgc aacagtaatg 181 tgtttgttcg tctgctgaga ctcggctcca aggtcacagc tcgtaacact ggtgttccat 241 tgccTAAAGA GGAGAACATT TCTGCTCCCT TGATCTCCAG CTCCCCCAGT GAAGGCAGCC 301 CGGGAGTATG AAGATCCCCC TAGTGAAGAG GAAGATAAAA TAAAAGAAGA ACCTTTAACC 361 ATCTCTGAAC TAGTGTACAA CCCAAGTGCC AGCCTGCTCC CCACCCCTGT GGATGACGAT 421 GAGATTGACA TGCTCTTTGA CTGTCCTTCT AGGCTTGAGT TGGAAAGAGA AGACACAGAT 481 TCATTTGAGG ATCTGGAAGC AGATGAAAAC GCCTTTTTGA TTGCTGAAGA AGAGGAGCTG 541 AAGGAGGCTC GCCGTGCTTT GTCGTGGAGC TATGACATTC TGACTGGCCA TATTCGGGTG 601 AACCCACTGG TCAAGAGTTT TTCCAGGCTC CTTGTGGTGG GCCTGGGACT GCTGCTCTTT 661 GTATTTCCCC TGCTCCTCCT CCTTTTGGAG TCAGTCTCCA TGCAGTACCC AACTTTCTTG 721 TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG 781 TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT 841 GGAAAGGACG ACGACCCCTC CCCAGTTATG TTCCAACGCG TTAAGTCgac aatcaacctc 901 tggattacaa aatttgtgaa agatt