Construct: ORF TRCN0000479122
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011781.1_s317c1
- Derived from:
- ccsbBroadEn_06338
- DNA Barcode:
- GATCCGATCTACCTAATTGTCTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GTF2A2 (2958)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479122
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2958 | GTF2A2 | general transcription facto... | NM_001320929.2 | 99.6% | 100% | 315C>T |
| 2 | human | 2958 | GTF2A2 | general transcription facto... | NM_001320930.2 | 99.6% | 100% | 315C>T |
| 3 | human | 2958 | GTF2A2 | general transcription facto... | NM_004492.3 | 99.6% | 100% | 315C>T |
| 4 | mouse | 235459 | Gtf2a2 | general transcription facto... | NM_001039519.2 | 95.1% | 99% | (many diffs) |
| 5 | mouse | 235459 | Gtf2a2 | general transcription facto... | NM_001282083.1 | 95.1% | 99% | (many diffs) |
| 6 | mouse | 235459 | Gtf2a2 | general transcription facto... | XM_006511116.2 | 95.1% | 99% | (many diffs) |
| 7 | mouse | 235459 | Gtf2a2 | general transcription facto... | NM_001282084.1 | 55.7% | 55% | (many diffs) |
| 8 | mouse | 235459 | Gtf2a2 | general transcription facto... | NR_104093.1 | 30% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 417
- ORF length:
- 327
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGCATATCA GTTATACAGA AATACTACTT 121 TGGGAAACAG TCTTCAGGAG AGCCTAGATG AGCTCATACA GTCTCAACAG ATCACCCCCC 181 AACTTGCCCT TCAAGTTCTA CTTCAGTTTG ATAAGGCTAT AAATGCAGCA CTGGCTCAGA 241 GGGTCAGGAA CAGAGTCAAT TTCAGGGGCT CTCTAAATAC GTACAGATTC TGCGATAATG 301 TGTGGACTTT TGTACTGAAT GATGTTGAAT TCAGAGAGGT GACAGAACTT ATTAAAGTGG 361 ATAAAGTGAA AATTGTAGCC TGTGATGGTA AAAATACTGG CTCTAATACT ACAGAATGCC 421 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 481 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 541 ATATATCTTG TGGAAAGGAC GAGATCCGAT CTACCTAATT GTCTCCACGC GTTAAGTCga 601 caatcaacct ctggattaca aaatttgtga aagatt