Construct: ORF TRCN0000479142
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003423.1_s317c1
- Derived from:
- ccsbBroadEn_01492
- DNA Barcode:
- CGTCCACGGCTTCAGTATCCTTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SCNN1G (6340)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479142
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6340 | SCNN1G | sodium channel epithelial 1... | NM_001039.4 | 100% | 100% | |
| 2 | mouse | 20278 | Scnn1g | sodium channel, nonvoltage-... | NM_011326.3 | 83.8% | 85.4% | (many diffs) |
| 3 | mouse | 20278 | Scnn1g | sodium channel, nonvoltage-... | XM_006507477.4 | 66.5% | 67.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2013
- ORF length:
- 1947
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc acccggagag aagatcaaag ccaaaatcaa gaagaatctg cccgtgacgg 121 gccctcaggc gccgaccatt aaagagctga tgcggtggta ctgcctcaac accaacaccc 181 atggctgtcg ccgcatcgtg gtgtcccgcg gccgtctgcg ccgcctcctc tggatcgggt 241 tcacactgac tgccgtggcc ctcatcctct ggcagtgcgc cctcctcgtc ttctccttct 301 atactgtctc agtttccatc aaagtccact tccggaagct ggattttcct gcagtcacca 361 tctgcaacat caacccctac aagtacagca ccgttcgcca ccttctagct gacttggaac 421 aggagaccag agaggccctg aagtccctgt atggctttcc agagtcccgg aagcgccgag 481 aggcggagtc ctggaactcc gtctcagagg gaaagcagcc tagattctcc caccggattc 541 cgctgctgat ctttgatcag gatgagaagg gcaaggccag ggacttcttc acagggagga 601 agcggaaagt cggcggtagc atcattcaca aggcttcaaa tgtcatgcac atcgagtcca 661 agcaagtggt gggattccaa ctgtgctcaa atgacacctc cgactgtgcc acctacacct 721 tcagctcggg aatcaatgcc attcaggagt ggtataagct acactacatg aacatcatgg 781 cacaggtgcc tctggagaag aaaatcaaca tgagctattc tgctgaggag ctgctggtga 841 cctgcttctt tgatggagtg tcctgtgatg ccaggaattt cacgcttttc caccacccga 901 tgcatgggaa ttgctatact ttcaacaaca gagaaaatga gaccattctc agcacctcca 961 tggggggcag cgaatatggg ctgcaagtca ttttgtacat aaacgaagag gaatacaacc 1021 cattcctcgt gtcctccact ggagctaagg tgatcatcca tcggcaggat gagtatccct 1081 tcgtcgaaga tgtgggaaca gagattgaga cagcaatggt cacctctata ggaatgcacc 1141 tgacagagtc cttcaagctg agtgagccct acagtcagtg cacggaggac gggagtgacg 1201 tgccaatcag gaacatctac aacgctgcct actcgctcca gatctgcctt cattcatgct 1261 tccagacaaa gatggtggag aaatgtgggt gtgcccagta cagccagcct ctacctcctg 1321 cagccaacta ctgcaactac cagcagcacc ccaactggat gtattgttac taccaactgc 1381 atcgagcctt tgtccaggaa gagctgggct gccagtctgt gtgcaaggaa gcctgcagct 1441 ttaaagagtg gacactaacc acaagcctgg cacaatggcc atctgtggtt tcggagaagt 1501 ggttgctgcc tgttctcact tgggaccaag gccggcaagt aaacaaaaag ctcaacaaga 1561 cagacttggc caaactcttg atattctaca aagacctgaa CCAGAGATCC ATCATGGAGA 1621 GCCCAGCCAA CAGTATTGAG ATGCTTCTGT CCAACTTCGG TGGCCAGCTG GGCCTGTGGA 1681 TGAGCTGCTC TGTTGTCTGC GTCATCGAGA TCATCGAGGT CTTCTTCATT GACTTCTTCT 1741 CTATCATTGC CCGCCGCCAG TGGCAGAAAG CCAAGGAGTG GTGGGCCTGG AAACAGGCTC 1801 CCCCATGTCC AGAAGCTCCC CGTAGCCCAC AGGGCCAGGA CAATCCAGCC CTGGATATAG 1861 ACGATGACCT ACCCACTTTC AACTCTGCTT TGCACCTGCC TCCAGCCCTA GGAACCCAAG 1921 TGCCCGGCAC ACCGCCCCCC AAATACAATA CCTTGCGCTT GGAGAGGGCC TTTTCCAACC 1981 AGCTCACAGA TACCCAGATG CTGGATGAGC TCTGCCCAAC TTTCTTGTAC AAAGTGGTTG 2041 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2101 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACG 2161 TCCACGGCTT CAGTATCCTT CAACGCGTTA AGTCgacaat caacctctgg attacaaaat 2221 ttgtgaaaga tt