Construct: ORF TRCN0000479290

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010701.1_s317c1
Derived from:
ccsbBroadEn_09435
DNA Barcode:
TATCCTCAAGCTTACCCGAACAGT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HSPA12B (116835)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000479290
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 116835 HSPA12B heat shock protein family A... NM_052970.5 99.9% 100% 1881C>T
2 human 116835 HSPA12B heat shock protein family A... XM_017027632.2 99.9% 100% 1881C>T
3 human 116835 HSPA12B heat shock protein family A... NM_001197327.2 99.8% 99.8% 556_557insAGG;1878C>T
4 human 116835 HSPA12B heat shock protein family A... NM_001318322.2 87.4% 87.4% 0_1ins258;1623C>T
5 human 116835 HSPA12B heat shock protein family A... XM_011529151.2 75.7% 75.8% 0_1ins498;1383C>T
6 human 116835 HSPA12B heat shock protein family A... XM_017027633.1 71.1% 68.1% (many diffs)
7 human 116835 HSPA12B heat shock protein family A... XM_017027634.1 68.4% 68.2% 1406_1406delGinsAGGCCC;1409T>C;1410_1411ins643
8 human 116835 HSPA12B heat shock protein family A... XM_017027635.1 68.4% 68.2% 1406_1406delGinsAGGCCC;1409T>C;1410_1411ins643
9 mouse 72630 Hspa12b heat shock protein 12B NM_028306.3 85.7% 93.8% (many diffs)
10 mouse 72630 Hspa12b heat shock protein 12B XM_011239793.2 61.2% 67% (many diffs)
11 mouse 72630 Hspa12b heat shock protein 12B XM_006500245.3 53.4% 58.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2127
ORF length:
2058
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gttggctgtc ccggagatgg gcctgcaggg gctgtacatc ggctccagcc
121cggagcggtc cccagtgcct agcccacccg gctccccgag gacccaggaa agctgcggca
181ttgcccccct cacaccctcg cagtctccaa aacccgaggt ccgagccccc cagcaggcct
241ccttctctgt ggtggtggcc attgacttcg gcaccacgtc tagtggctat gctttcagct
301ttgccagtga ccctgaggcc atccacatga tgaggaaatg ggagggcgga gacccgggcg
361tggcccacca gaagaccccg acctgcctgc tgctgactcc ggagggcgcc ttccacagct
421ttggctacac cgcccgcgat tactaccatg acctggaccc cgaagaggcg cgggactggc
481tctacttcga gaagttcaag atgaagatcc acagcgccac ggatctcacc ttgaagaccc
541agctagaggc agtaaatgga aagacgatgc ccgccctgga ggtgttcgcc catgccctgc
601gcttcttcag ggagcacgcc cttcaggagc tgagggagca gagcccatcg ctgccagaga
661aggacactgt gcgctgggtg ttgacggtgc ctgccatctg gaaacagcca gccaagcagt
721tcatgcggga ggctgcctac ctggctggac tagtgtcccg agagaatgca gagcagctac
781tcatcgccct ggagcccgag gccgcctcgg tatactgccg caagctgcgc ctgcaccagc
841tcctggacct gagtggccgg gccccaggtg gtgggcgcct gggtgagcgc cgctccatcg
901actccagctt ccgtcaggct cgggagcagc tgcgaaggtc ccgccacagc cgcacgttcc
961tggtggagtc aggcgtagga gagctgtggg cagagatgca agcaggagac cgctacgtgg
1021tggccgactg cggcggaggc accgtggacc tgacggtgca ccagctggag cagccccatg
1081gcaccctcaa ggagctctac aaggcatctg ggggccctta tggcgcggtg ggcgtggacc
1141tggccttcga gcagctgctg tgccgcatct tcggcgagga cttcatcgcc accttcaaaa
1201ggcaacggcc ggcagcctgg gtagatctga ccatcgcctt cgaggctcgc aagcgcactg
1261ctggcccaca ccgtgcaggg gcgctcaaca tctcgctgcc cttctccttc attgacttct
1321accgcaagca gcggggccac aacgtggaga ccgctctgcg caggagcagc gtgaacttcg
1381tgaagtggtc ctcacagggg atgctccgaa tgtcttgtga agccatgaac gagctctttc
1441agcccaccgt cagcgggatc atccagcaca tagaggccct gctggcacgg ccggaggtgc
1501agggtgtgaa gctgctgttc ctagtgggcg gcttcgccga gtcagcggtg ctgcagcacg
1561cggtgcaggc ggcgctgggc gcccgcggtc tgcgtgtcgt ggtcccgcac gacgtgggcc
1621tcaccatcct caaaggcgcg gtgctgttcg gccaggcgcc gggcgtggtg cgggtccgcc
1681gctcgccgct caccTATGGC GTGGGCGTGC TCAACCGCTT TGTGCCTGGG CGCCACCCGC
1741CCGAAAAGCT GCTGGTTCGC GACGGCCGCC GCTGGTGCAC CGACGTCTTC GAGCGCTTCG
1801TGGCCGCCGA GCAGTCGGTG GCCCTGGGCG AGGAGGTGCG GCGCAGCTAC TGCCCGGCGC
1861GTCCCGGCCA GCGGCGCGTA CTCATCAACC TGTACTGCTG CGCGGCAGAG GATGCGCGCT
1921TCATCACCGA CCCCGGCGTG CGCAAATGTG GCGCGCTCAG CCTCGAGCTT GAGCCCGCCG
1981ACTGCGGCCA GGACACCGCC GGCGCGCCTC CCGGCCGCCG CGAGATCCGC GCCGCCATGC
2041AGTTTGGCGA CACCGAAATT AAGGTCACCG CCGTCGACGT CAGCACCAAT CGCTCCGTGC
2101GCGCGTCCAT CGACTTTCTT TCCAACTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG
2161GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
2221AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATATCCTCA
2281AGCTTACCCG AACAGTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
2341aagatt

Download FASTA (ORF) (Full)