Construct: ORF TRCN0000479290
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010701.1_s317c1
- Derived from:
- ccsbBroadEn_09435
- DNA Barcode:
- TATCCTCAAGCTTACCCGAACAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HSPA12B (116835)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479290
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 116835 | HSPA12B | heat shock protein family A... | NM_052970.5 | 99.9% | 100% | 1881C>T |
| 2 | human | 116835 | HSPA12B | heat shock protein family A... | XM_017027632.2 | 99.9% | 100% | 1881C>T |
| 3 | human | 116835 | HSPA12B | heat shock protein family A... | NM_001197327.2 | 99.8% | 99.8% | 556_557insAGG;1878C>T |
| 4 | human | 116835 | HSPA12B | heat shock protein family A... | NM_001318322.2 | 87.4% | 87.4% | 0_1ins258;1623C>T |
| 5 | human | 116835 | HSPA12B | heat shock protein family A... | XM_011529151.2 | 75.7% | 75.8% | 0_1ins498;1383C>T |
| 6 | human | 116835 | HSPA12B | heat shock protein family A... | XM_017027633.1 | 71.1% | 68.1% | (many diffs) |
| 7 | human | 116835 | HSPA12B | heat shock protein family A... | XM_017027634.1 | 68.4% | 68.2% | 1406_1406delGinsAGGCCC;1409T>C;1410_1411ins643 |
| 8 | human | 116835 | HSPA12B | heat shock protein family A... | XM_017027635.1 | 68.4% | 68.2% | 1406_1406delGinsAGGCCC;1409T>C;1410_1411ins643 |
| 9 | mouse | 72630 | Hspa12b | heat shock protein 12B | NM_028306.3 | 85.7% | 93.8% | (many diffs) |
| 10 | mouse | 72630 | Hspa12b | heat shock protein 12B | XM_011239793.2 | 61.2% | 67% | (many diffs) |
| 11 | mouse | 72630 | Hspa12b | heat shock protein 12B | XM_006500245.3 | 53.4% | 58.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2127
- ORF length:
- 2058
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gttggctgtc ccggagatgg gcctgcaggg gctgtacatc ggctccagcc 121 cggagcggtc cccagtgcct agcccacccg gctccccgag gacccaggaa agctgcggca 181 ttgcccccct cacaccctcg cagtctccaa aacccgaggt ccgagccccc cagcaggcct 241 ccttctctgt ggtggtggcc attgacttcg gcaccacgtc tagtggctat gctttcagct 301 ttgccagtga ccctgaggcc atccacatga tgaggaaatg ggagggcgga gacccgggcg 361 tggcccacca gaagaccccg acctgcctgc tgctgactcc ggagggcgcc ttccacagct 421 ttggctacac cgcccgcgat tactaccatg acctggaccc cgaagaggcg cgggactggc 481 tctacttcga gaagttcaag atgaagatcc acagcgccac ggatctcacc ttgaagaccc 541 agctagaggc agtaaatgga aagacgatgc ccgccctgga ggtgttcgcc catgccctgc 601 gcttcttcag ggagcacgcc cttcaggagc tgagggagca gagcccatcg ctgccagaga 661 aggacactgt gcgctgggtg ttgacggtgc ctgccatctg gaaacagcca gccaagcagt 721 tcatgcggga ggctgcctac ctggctggac tagtgtcccg agagaatgca gagcagctac 781 tcatcgccct ggagcccgag gccgcctcgg tatactgccg caagctgcgc ctgcaccagc 841 tcctggacct gagtggccgg gccccaggtg gtgggcgcct gggtgagcgc cgctccatcg 901 actccagctt ccgtcaggct cgggagcagc tgcgaaggtc ccgccacagc cgcacgttcc 961 tggtggagtc aggcgtagga gagctgtggg cagagatgca agcaggagac cgctacgtgg 1021 tggccgactg cggcggaggc accgtggacc tgacggtgca ccagctggag cagccccatg 1081 gcaccctcaa ggagctctac aaggcatctg ggggccctta tggcgcggtg ggcgtggacc 1141 tggccttcga gcagctgctg tgccgcatct tcggcgagga cttcatcgcc accttcaaaa 1201 ggcaacggcc ggcagcctgg gtagatctga ccatcgcctt cgaggctcgc aagcgcactg 1261 ctggcccaca ccgtgcaggg gcgctcaaca tctcgctgcc cttctccttc attgacttct 1321 accgcaagca gcggggccac aacgtggaga ccgctctgcg caggagcagc gtgaacttcg 1381 tgaagtggtc ctcacagggg atgctccgaa tgtcttgtga agccatgaac gagctctttc 1441 agcccaccgt cagcgggatc atccagcaca tagaggccct gctggcacgg ccggaggtgc 1501 agggtgtgaa gctgctgttc ctagtgggcg gcttcgccga gtcagcggtg ctgcagcacg 1561 cggtgcaggc ggcgctgggc gcccgcggtc tgcgtgtcgt ggtcccgcac gacgtgggcc 1621 tcaccatcct caaaggcgcg gtgctgttcg gccaggcgcc gggcgtggtg cgggtccgcc 1681 gctcgccgct caccTATGGC GTGGGCGTGC TCAACCGCTT TGTGCCTGGG CGCCACCCGC 1741 CCGAAAAGCT GCTGGTTCGC GACGGCCGCC GCTGGTGCAC CGACGTCTTC GAGCGCTTCG 1801 TGGCCGCCGA GCAGTCGGTG GCCCTGGGCG AGGAGGTGCG GCGCAGCTAC TGCCCGGCGC 1861 GTCCCGGCCA GCGGCGCGTA CTCATCAACC TGTACTGCTG CGCGGCAGAG GATGCGCGCT 1921 TCATCACCGA CCCCGGCGTG CGCAAATGTG GCGCGCTCAG CCTCGAGCTT GAGCCCGCCG 1981 ACTGCGGCCA GGACACCGCC GGCGCGCCTC CCGGCCGCCG CGAGATCCGC GCCGCCATGC 2041 AGTTTGGCGA CACCGAAATT AAGGTCACCG CCGTCGACGT CAGCACCAAT CGCTCCGTGC 2101 GCGCGTCCAT CGACTTTCTT TCCAACTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 2161 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 2221 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATATCCTCA 2281 AGCTTACCCG AACAGTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 2341 aagatt