Construct: ORF TRCN0000479298
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003960.1_s317c1
- Derived from:
- ccsbBroadEn_07846
- DNA Barcode:
- ATAGCGTGTATTCGTCGCACCGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAPK8IP3 (23162)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479298
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23162 | MAPK8IP3 | mitogen-activated protein k... | NM_015133.5 | 99.9% | 100% | 2226C>A;3765G>C |
| 2 | human | 23162 | MAPK8IP3 | mitogen-activated protein k... | NM_001318852.1 | 99.8% | 99.9% | 601_603delAGC;2229C>A;3768G>C |
| 3 | human | 23162 | MAPK8IP3 | mitogen-activated protein k... | NM_001040439.2 | 99.5% | 99.5% | 1224_1225insGTGCGCGATGATTTCTTT;2208C>A;3747G>C |
| 4 | human | 23162 | MAPK8IP3 | mitogen-activated protein k... | XM_011522429.2 | 99.2% | 99.3% | 601_627del;2253C>A;3792G>C |
| 5 | human | 23162 | MAPK8IP3 | mitogen-activated protein k... | XM_011522430.2 | 98.8% | 98.8% | (many diffs) |
| 6 | human | 23162 | MAPK8IP3 | mitogen-activated protein k... | XM_005255190.2 | 98.7% | 98.8% | (many diffs) |
| 7 | human | 23162 | MAPK8IP3 | mitogen-activated protein k... | XM_024450204.1 | 96.3% | 96.3% | 2226C>A;3521_3670del;3915G>C |
| 8 | human | 23162 | MAPK8IP3 | mitogen-activated protein k... | XM_024450203.1 | 96.2% | 96.2% | (many diffs) |
| 9 | human | 23162 | MAPK8IP3 | mitogen-activated protein k... | XM_024450206.1 | 95.8% | 95.8% | (many diffs) |
| 10 | human | 23162 | MAPK8IP3 | mitogen-activated protein k... | XM_024450201.1 | 95.7% | 95.6% | (many diffs) |
| 11 | human | 23162 | MAPK8IP3 | mitogen-activated protein k... | XM_024450208.1 | 95.6% | 95.6% | (many diffs) |
| 12 | human | 23162 | MAPK8IP3 | mitogen-activated protein k... | XM_024450202.1 | 95.2% | 95.2% | (many diffs) |
| 13 | human | 23162 | MAPK8IP3 | mitogen-activated protein k... | XM_024450205.1 | 95% | 95% | (many diffs) |
| 14 | human | 23162 | MAPK8IP3 | mitogen-activated protein k... | XM_024450207.1 | 94.6% | 94.6% | (many diffs) |
| 15 | human | 23162 | MAPK8IP3 | mitogen-activated protein k... | XM_024450209.1 | 83.8% | 83.5% | (many diffs) |
| 16 | human | 23162 | MAPK8IP3 | mitogen-activated protein k... | XM_024450210.1 | 83.1% | 77.3% | (many diffs) |
| 17 | human | 23162 | MAPK8IP3 | mitogen-activated protein k... | XM_024450211.1 | 78% | 78% | (many diffs) |
| 18 | human | 23162 | MAPK8IP3 | mitogen-activated protein k... | XR_001751875.1 | 72.3% | (many diffs) | |
| 19 | mouse | 30957 | Mapk8ip3 | mitogen-activated protein k... | NM_001163447.1 | 86.4% | 93.7% | (many diffs) |
| 20 | mouse | 30957 | Mapk8ip3 | mitogen-activated protein k... | NM_013931.4 | 86.3% | 93.7% | (many diffs) |
| 21 | mouse | 30957 | Mapk8ip3 | mitogen-activated protein k... | NM_001163448.1 | 85.3% | 92.6% | (many diffs) |
| 22 | mouse | 30957 | Mapk8ip3 | mitogen-activated protein k... | NM_001163451.1 | 84.1% | 91.5% | (many diffs) |
| 23 | mouse | 30957 | Mapk8ip3 | mitogen-activated protein k... | NM_001163450.1 | 84.1% | 91.4% | (many diffs) |
| 24 | mouse | 30957 | Mapk8ip3 | mitogen-activated protein k... | NM_001163453.1 | 83.6% | 91% | (many diffs) |
| 25 | mouse | 30957 | Mapk8ip3 | mitogen-activated protein k... | NM_001163449.1 | 83.6% | 90.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 4077
- ORF length:
- 4008
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gatggagatc cagatggacg agggcggcgg cgtggtggtg taccaggacg 121 actactgctc cggctcggtg atgtcggagc gggtgtcggg cctggcgggc tccatctacc 181 gcgagttcga gcgcctcatc cactgctacg acgaggaggt ggtcaaggag ctcatgccgc 241 tggtggtgaa cgtgctggag aacctagact cggtgctcag cgagaaccag gagcacgagg 301 tggagctgga gctgctgcgc gaggacaacg agcagctgct cacccagtac gagcgtgaga 361 aggcgctgcg caggcaggcg gaggagaaat tcattgagtt tgaagatgct ctggaacaag 421 agaagaaaga gctgcaaatc caggtggagc actacgagtt ccagacgcgc cagctggagc 481 tgaaggccaa gaactatgcc gatcagattt cccggttgga ggagcgggag tcggagatga 541 agaaggagta caatgccctg caccagcggc acacagagat gatacagacc tacgtggagc 601 acattgagag gtccaagatg cagcaggtcg gaggaaacag ccagaccgag agcagcctgc 661 cggggcggag gaaggagcgc cccacctccc tgaacgtgtt ccccctggct gacggcacgg 721 tacgtgcaca gatcgggggc aagctcgtgc ctgcggggga ccactggcac ctgagtgacc 781 tcggccagct gcagtccagc tccagctacc agtgtccaca ggatgaaatg tccgagtcag 841 gccagtcctc ggcggccgcc acacccagca ccacaggcac caagtccaac acgcccacat 901 cctccgtgcc ctcggccgcc gtcacacccc tcaacgagag cctgcagccc ctgggggact 961 atggcgtggg ctccaagaac agcaagcgtg cccgggagaa gcgcgacagc cgcaacatgg 1021 aagtacaggt cacccaggag atgcgcaacg tcagtatagg catgggcagc agtgacgagt 1081 ggtctgatgt tcaagacatt attgactcca cgccagagct ggacatgtgt ccagagaccc 1141 gcctggaccg cacaggaagc agcccaaccc agggcatcgt gaacaaagct ttcggcatca 1201 acaccgactc cctgtaccat gagctgtcga cggcagggtc tgaggtcatc ggggatgtgg 1261 acgaaggggc cgacctccta ggggagttct cagtgcgcga tgatttcttt ggaatgggca 1321 aagaagtggg gaatctgcta ctggaaaact cacagcttct ggaaaccaaa aacgccttga 1381 atgtggtgaa gaatgacctg attgccaagg tcgaccagct gtccggggag caggaggtgc 1441 tgaggggcga gttggaggct gctaagcagg ccaaagtcaa gctggaaaac cgtatcaagg 1501 agctggaaga ggaactgaaa agagtgaagt ccgaggccat catcgcccgc cgtgaaccca 1561 aagaagaggc ggaggatgta agcagctatc tctgtacaga atcggacaaa atccccatgg 1621 cccagcgccg ccgcttcacg cgggtggaga tggcccgtgt gctcatggag cggaaccagt 1681 acaaggagcg gctgatggag ctgcaggagg ctgtgcggtg gactgagatg atcagagcgt 1741 cccgagagca cccatccgtc caggagaaga agaagtcgac catctggcag ttcttcagcc 1801 gcctcttcag ctcttcctcc agcccccctc cggccaagcg cccctatccc tcggtgaaca 1861 tccactacaa gtcacccacc actgccggct tcagccagcg ccgcaaccat gccatgtgcc 1921 cgatctcggc aggcagccgg cccctggaat tcttccctga cgacgactgc acgtcctccg 1981 cccgtcgaga gcagaagcgc gagcagtacc gccaggtgcg tgagcacgtg cgtaacgacg 2041 acggccgtct gcaggcctgc ggctggagcc tgcccgccaa gtacaagcag ctgagtccca 2101 acgggggcca ggaggacacg cggatgaaga acgtgccggt gccggtgtac tgccgccctc 2161 tggtggagaa ggaccccacc atgaagctgt ggtgtgccgc gggcgtcaac ctgagcgggt 2221 ggaggcccaa tgaggacgac gctgggaatg gagtcaagcc agcgccaggc cgcgatcccc 2281 tgacctgcga ccgagaagga gacggcgagc ccaagagcgc ccacacgtct cccgagaaga 2341 agaaggccaa ggagctccct gaaatggacg ccacctccag ccgggtgtgg atcctgacca 2401 gcaccctgac caccagcaag gtggtgatca tcgacgccaa ccagccgggc acggtggtgg 2461 accagttcac cgtctgcaac gcgcacgtgc tgtgcatctc cagcatcccc gcggccagcg 2521 acagcgacta ccctcccggg gagatgttcc tggacagcga cgtgaaccca gaggacccgg 2581 gcgcagatgg cgtgctggcc ggtatcaccc tggtgggctg tgccacccgc tgcaacgtgc 2641 cgcggagcaa ctgctcctcc cgaggggaca ccccagtgct agacaagggg cagggggagg 2701 tggccaccat cgccaacggg aaggtcaacc cgtcccagtc cacagaggag gccacagagg 2761 ccacggaggt gccagaccct gggcccagcg agccagagac agccacattg cggcccgggc 2821 ctctcacaga gcacgtcttc actgacccag ccccgacccc gtcctctggc ccccagcctg 2881 gcagcgagaa cgggccagag cctgacagca gcagcacacg gccagagcca gagcccagcg 2941 gggaccccac gggagcaggc agcagtgctg cacccaccat gtggctggga gcccagaacg 3001 gctggctcta tgtgcactcg gctgtggcca actggaagaa gtgcctgcac tccatcaagc 3061 tgaaggattc tgtgctgagc ctggtgcatg tcaaaggccg tgtgctggtg gctctggcgg 3121 acgggaccct ggccatcttc caccgtggtg aagatggcca gtgggatctg agcaactatc 3181 acctaatgga cctgggccac ccgcaccact ccatccgctg catggctgtt gtgtacgacc 3241 gcgtgtggtg tggctacaag aacaaggtgc acgtcatcca gcccaagacc atgcagatag 3301 agaagtcatt tgacgcccac ccgcggcggg agagccaggt gcggcagctg gcgtggatcg 3361 gcgatggcgt atgggtgtcc atccgcctgg actccaccct gaggctctac catgcacaca 3421 cgcaccagca tctacaggac gtggacattg agccctacgt cagcaagatg ctaggcactg 3481 gcaagctggg tttctccttc gtacgcatca cggccctgct tgtcgcgggc agccggctct 3541 gggtgggcac cggcaacgga gtggtcatct ccatccccct gacagagact gtggtcctgc 3601 accgaggcca gctcctgggg ctccgagcca ataagacatc ccccacctct ggggagggcg 3661 cccgtcccgg gggcatcatc cacgtgtatg gcgatgacag cagtgacagg gcggccagca 3721 gcttcaTCCC CTACTGCTCC ATGGCCCAGG CCCAGCTATG CTTCCATGGG CACCGCGATG 3781 CCGTGAAGTT CTTTGTCTCG GTGCCAGGGA ACGTGCTGGC CACCCTGAAT GGCAGTGTGC 3841 TGGACAGCCC AGCCGAGGGC CCTGGGCCAG CTGCCCCTGC CTCGGAGGTC GAGGGCCAGA 3901 AGCTGCGGAA CGTGCTGGTG CTGAGCGGCG GGGAGGGCTA CATCGACTTC CGCATTGGAG 3961 ACGGAGAGGA CGACGAGACG GAGGAGGGCG CAGGGGACAT GAGCCAGGTG AAGCCCGTGC 4021 TGTCCAAGGC AGAGCGCAGT CACATCATCG TGTGGCAGGT GTCCTACACC CCCGAGTTGC 4081 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 4141 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 4201 ATATATCTTG TGGAAAGGAC GAATAGCGTG TATTCGTCGC ACCGGTACGC GTTAAGTCga 4261 caatcaacct ctggattaca aaatttgtga aagatt