Construct: ORF TRCN0000479310
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000630.1_s317c1
- Derived from:
- ccsbBroadEn_09694
- DNA Barcode:
- TAACTTGGCACGGGCCATGCGCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CREBRF (153222)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479310
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 153222 | CREBRF | CREB3 regulatory factor | NM_153607.3 | 99.9% | 99.8% | 1447A>G |
| 2 | human | 153222 | CREBRF | CREB3 regulatory factor | XM_006714822.4 | 99.9% | 99.8% | 1447A>G |
| 3 | human | 153222 | CREBRF | CREB3 regulatory factor | XM_005265821.3 | 98.6% | 98.5% | 0_1ins24;1423A>G |
| 4 | human | 153222 | CREBRF | CREB3 regulatory factor | XM_011534442.1 | 98.6% | 98.5% | 0_1ins24;1423A>G |
| 5 | human | 153222 | CREBRF | CREB3 regulatory factor | NM_001168393.2 | 64.5% | 64% | (many diffs) |
| 6 | human | 153222 | CREBRF | CREB3 regulatory factor | NM_001168394.2 | 64.5% | 64% | (many diffs) |
| 7 | mouse | 77128 | Crebrf | CREB3 regulatory factor | NM_029870.2 | 89.6% | 93.5% | (many diffs) |
| 8 | mouse | 77128 | Crebrf | CREB3 regulatory factor | XM_006525114.2 | 89.6% | 93.5% | (many diffs) |
| 9 | mouse | 77128 | Crebrf | CREB3 regulatory factor | XM_011246717.2 | 89.6% | 93.5% | (many diffs) |
| 10 | mouse | 77128 | Crebrf | CREB3 regulatory factor | XM_017317723.1 | 89.6% | 93.5% | (many diffs) |
| 11 | mouse | 77128 | Crebrf | CREB3 regulatory factor | XM_017317724.1 | 88.5% | 92.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1983
- ORF length:
- 1917
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc tcagcctagt gtaagcggaa tggatccgcc tttcggggat gcctttcgaa 121 gccacacctt ttcggaacaa actctgatga gcacagatct cttagcaaac agttcggatc 181 cagatttcat gtatgaactg gatagagaga tgaactacca acagaatcct agagacaact 241 ttctttcttt ggaggactgc aaagacattg aaaatctgga gtctttcaca gatgtcctgg 301 ataatgaggg tgctttaacc tcaaactggg aacagtggga tacatactgt gaagacctaa 361 cgaaatatac caaactaacc agctgtgaca tctggggaac aaaagaagtg gattacttgg 421 gtcttgatga cttttctagt ccttaccaag atgaagaggt tataagtaaa actccaactt 481 tagctcaact taatagtgag gactcacagt ctgtttctga ttccctttat taccccgatt 541 cacttttcag tgtcaaacaa aatcccttac cctcttcatt ccctggtaaa aagatcacaa 601 gcagagcagc tgctcctgtg tgttcttcta agactctgca ggctgaggtc cctttgtcag 661 actgtgtcca aaaagcaagt aaacccactt caagcacaca aatcatggtg aagaccaaca 721 tgtatcataa tgaaaaggtg aactttcatg ttgaatgtaa agactatgta aaaaaggcaa 781 aggtaaagat caacccagtg caacagagcc ggcccttgtt gagccagatt cacacagatg 841 cagcaaagga gaacacctgc tactgtggtg cagtggcaaa gagacaagag aaaaaaggga 901 tggagcctct tcaaggtcat gccactcccg ctttgccttt taaagaaacc caggaactat 961 tactaagtcc cctgccccag gaaggtcctg ggtcacttgc agcaggagag agcagcagtc 1021 tttctgccag tacatcagtc tcagattcat cccagaaaaa agaagagcac aattattctc 1081 tttttgtctc cgacaacttg ggtgaacagc caactaaatg cagtcctgaa gaagatgagg 1141 aggacgagga ggatgttgat gatgaggacc atgatgaagg attcggcagt gagcatgaac 1201 tgtctgaaaa tgaggaggag gaagaagagg aagaggatta tgaagatgac aaggatgatg 1261 atattagtga tactttctct gaaccaggct atgaaaatga ttctgtagaa gacctgaagg 1321 aggtgacttc aatatcttca cggaagagag gtaaaagaag atacttctgg gagtatagtg 1381 aacaacttac accatcacag caagagagga tgctgagacc atctgagtgg aaccgagata 1441 ctttgccaag taatatgtat cagaaaaatg gcttacatca tggaaaatat gcagtaaaga 1501 agtcacggag agctgatgta gaagacctga ctccaaaTCC TAAAAAACTC CTCCAGATAG 1561 GCAATGAACT TCGGAAACTG AATAAGGTGA TTAGTGACCT GACTCCAGTC AGTGAGCTTC 1621 CCTTAACAGC CCGACCAAGG TCAAGGAAGG AAAAAAATAA GCTGGCTTCC AGAGCTTGTC 1681 GGTTAAAGAA GAAAGCCCAG TATGAAGCTA ATAAAGTGAA ATTATGGGGC CTCAACACAG 1741 AATATGATAA TTTATTGTTT GTAATCAACT CCATCAAGCA AGAGATTGTA AACCGGGTAC 1801 AGAATCCAAG AGATGAGAGA GGACCCAACA TGGGGCAGAA GCTTGAAATC CTCATTAAAG 1861 ATACTCTCGG TCTACCAGTT GCTGGGCAAA CCTCAGAATT TGTTAACCAA GTGTTAGAGA 1921 AGACTGCAGA AGGGAATCCC ACTGGAGGCC TTGTAGGATT AAGGATACCA ACATCAAAGG 1981 TGTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 2041 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2101 GGCTTTATAT ATCTTGTGGA AAGGACGATA ACTTGGCACG GGCCATGCGC CCACGCGTTA 2161 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt