Construct: ORF TRCN0000479405
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016756.1_s317c1
- Derived from:
- ccsbBroadEn_05774
- DNA Barcode:
- ATTTCCCCGTATGTGTGATTTTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADD2 (119)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479405
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 119 | ADD2 | adducin 2 | NM_001185054.1 | 99.9% | 99.8% | 1315A>G |
| 2 | human | 119 | ADD2 | adducin 2 | NM_001617.4 | 99.9% | 99.8% | 1315A>G |
| 3 | human | 119 | ADD2 | adducin 2 | XM_011532502.2 | 99.9% | 99.8% | 1315A>G |
| 4 | human | 119 | ADD2 | adducin 2 | NM_017488.4 | 81.3% | 80.3% | 1315A>G;1740_1825del;1929_1930ins335 |
| 5 | human | 119 | ADD2 | adducin 2 | NM_017482.4 | 75.2% | 74.3% | (many diffs) |
| 6 | human | 119 | ADD2 | adducin 2 | NM_001185055.2 | 73.6% | 72.7% | (many diffs) |
| 7 | mouse | 11519 | Add2 | adducin 2 (beta) | NM_001271857.1 | 86.9% | 92.1% | (many diffs) |
| 8 | mouse | 11519 | Add2 | adducin 2 (beta) | NM_001271858.1 | 86.9% | 92.1% | (many diffs) |
| 9 | mouse | 11519 | Add2 | adducin 2 (beta) | NM_001271859.1 | 86.9% | 92.1% | (many diffs) |
| 10 | mouse | 11519 | Add2 | adducin 2 (beta) | NM_013458.5 | 86.9% | 92.1% | (many diffs) |
| 11 | mouse | 11519 | Add2 | adducin 2 (beta) | NM_001271860.1 | 67.2% | 71.8% | (many diffs) |
| 12 | mouse | 11519 | Add2 | adducin 2 (beta) | NM_001271861.1 | 67.2% | 71.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2244
- ORF length:
- 2178
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag cgaagagacg gtccccgagg ctgcctcgcc gccgcccccg caggggcagc 121 cttactttga ccgcttctca gaggacgacc ccgagtacat gcgccttcgc aaccgggcgg 181 cggacctgcg gcaggacttc aacctgatgg agcagaagaa gcgcgtcacc atgatcctgc 241 agagtccctc tttcagggag gagctggaag gcctcatcca ggagcagatg aagaagggga 301 acaactcctc caacatctgg gccctgcgac agatcgcgga cttcatggcc agcacctccc 361 acgcagtctt cccgacatct tccatgaatg tctccatgat gacgcctatc aatgacctcc 421 acacagctga ctccctgaac ctggccaaag gggagcggct catgcggtgc aagatcagca 481 gtgtctaccg actcctggac ctctatggct gggcccagct gagtgacacc tatgtcacgt 541 tgagagtcag caaggagcag gaccacttcc tgatcagccc taagggagtt tcttgcagtg 601 aagtcacagc gtccagcctg atcaaggtga acattctggg agaggtggtg gagaagggca 661 gcagctgctt cccagtggac accacaggct tctgtctgca ctcggccatc tatgcagcga 721 ggcccgacgt gcgctgcatc atccacctgc acacaccggc cacagcagcg gtgtcggcca 781 tgaagtgggg cctcctgcct gtctcccaca atgccctgct ggtgggggac atggcctatt 841 atgacttcaa tggggaaatg gagcaggaag ccgatcggat caacctgcag aagtgccttg 901 gacccacctg caagatcctg gtgctaagaa accatggagt ggttgctctg ggtgacacgg 961 tagaggaggc attttacaag atcttccacc tgcaggctgc atgtgagata caggtgtcgg 1021 ctctgtccag tgccggggga gtggagaacc tcatcctcct ggagcaggag aagcaccggc 1081 cccatgaggt gggctccgtg cagtgggccg ggagcacctt tgggcctatg cagaagagtc 1141 ggctggggga gcatgagttt gaggccctca tgaggatgct ggacaacctg ggctacagaa 1201 caggttacac gtatcgccac ccctttgttc aagagaaaac caaacacaaa agtgaggtgg 1261 agattccagc cacggtcaca gccttcgtgt ttgaggagga cggtgccccg gtgcccgccc 1321 tgcgacagca tgcccagaag cagcagaagg agaagacccg ctggctcaat acgcccaacg 1381 cctacctgcg ggtcaatgtg gccgatgagg tccagaggag catgggcagc ccccgaccca 1441 agaccacgtg gatgaaggct gacgaggtgg agaaatccag cagtggcatg ccgattcgca 1501 tcgaaaaccc aaaccaattt gtgcctctct atactgaccc ccaggaagta ctggagatga 1561 ggaacaagat tcgagaacaa aaccgacaag atgtgaagtc agcggggcct cagtcccagc 1621 tcctggcgag cgtcattgcc gagaagagcc gaagcccgtc tacagagagc cagctgatgt 1681 ccaagggaga cgaggatacc aaagacgatt cagaggagac ggtgcccaac cccttcagcc 1741 aactcactga ccaggagttg gaggagtaca agaaagaggt ggagaggaag aaactagaac 1801 ttgatggaga gaaagaaact gccccagaag agcctggctc acctgcaaag tctgcacctg 1861 cttctccagt gcagagccca gcgaaggagg cagagacaaa gagcccttta gtctctcctt 1921 ccaagtcttt agaggaaggt actaagaaga cagaaacaag caaagccgcc accacagagc 1981 ccgaaacaac ccagccggaa ggggtggtgg tcaacgggag ggaggaggag cagacggcag 2041 aggaaatccT CAGCAAAGGC CTGAGCCAGA TGACCACCAG TGCTGACACG GATGTTGATA 2101 CCTCTAAGGA CAAAACCGAG TCGGTCACCA GCGGCCCCAT GTCCCCAGAG GGCTCACCTT 2161 CCAAGTCTCC CTCAAAGAAG AAAAAGAAAT TCCGAACCCC CTCCTTCCTG AAAAAGAGCA 2221 AAAAGAAGGA GAAAGTGGAG TCCTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 2281 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 2341 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA TTTCCCCGTA 2401 TGTGTGATTT TGAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 2461 att