Construct: ORF TRCN0000479440
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018990.3_s317c1
- Derived from:
- ccsbBroadEn_14637
- DNA Barcode:
- CCTAATGATTTCCCATTACAGGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FES (2242)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479440
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2242 | FES | FES proto-oncogene, tyrosin... | NM_002005.4 | 99.9% | 100% | 504T>C;2466delG |
| 2 | human | 2242 | FES | FES proto-oncogene, tyrosin... | XM_017022005.1 | 99.9% | 100% | 504T>C;2466delG |
| 3 | human | 2242 | FES | FES proto-oncogene, tyrosin... | XM_017022009.2 | 99.9% | 100% | 504T>C;2466delG |
| 4 | human | 2242 | FES | FES proto-oncogene, tyrosin... | NM_001143783.1 | 92.8% | 92.9% | 211_212ins174;330T>C;2292delG |
| 5 | human | 2242 | FES | FES proto-oncogene, tyrosin... | XM_005254880.1 | 92.8% | 92.9% | 211_212ins174;330T>C;2292delG |
| 6 | human | 2242 | FES | FES proto-oncogene, tyrosin... | XM_017022006.1 | 92.8% | 92.9% | 211_212ins174;330T>C;2292delG |
| 7 | human | 2242 | FES | FES proto-oncogene, tyrosin... | XM_017022010.1 | 92.8% | 92.9% | 211_212ins174;330T>C;2292delG |
| 8 | human | 2242 | FES | FES proto-oncogene, tyrosin... | NM_001143784.1 | 91.4% | 91.4% | 504T>C;1320_1321ins210;2256delG |
| 9 | human | 2242 | FES | FES proto-oncogene, tyrosin... | XM_005254882.2 | 91.4% | 91.4% | 504T>C;1320_1321ins210;2256delG |
| 10 | human | 2242 | FES | FES proto-oncogene, tyrosin... | XM_017022007.1 | 91.4% | 91.4% | 504T>C;1320_1321ins210;2256delG |
| 11 | human | 2242 | FES | FES proto-oncogene, tyrosin... | XR_001751142.1 | 87.9% | (many diffs) | |
| 12 | human | 2242 | FES | FES proto-oncogene, tyrosin... | XR_001751144.2 | 87% | (many diffs) | |
| 13 | human | 2242 | FES | FES proto-oncogene, tyrosin... | NM_001143785.2 | 84.3% | 84.4% | (many diffs) |
| 14 | human | 2242 | FES | FES proto-oncogene, tyrosin... | XM_017022008.1 | 84.3% | 84.4% | (many diffs) |
| 15 | human | 2242 | FES | FES proto-oncogene, tyrosin... | XR_001751143.1 | 80.4% | (many diffs) | |
| 16 | human | 2242 | FES | FES proto-oncogene, tyrosin... | XR_243206.3 | 79.6% | (many diffs) | |
| 17 | mouse | 14159 | Fes | feline sarcoma oncogene | NM_010194.2 | 87.2% | 90.6% | (many diffs) |
| 18 | mouse | 14159 | Fes | feline sarcoma oncogene | XM_006540604.3 | 87.2% | 90.6% | (many diffs) |
| 19 | mouse | 14159 | Fes | feline sarcoma oncogene | XM_006540605.2 | 48.7% | 43.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2535
- ORF length:
- 2466
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggcttctct tccgagctgt gcagccccca gggccacggg gtcctgcagc 121 aaatgcagga ggccgagctt cgtctactgg agggcatgag aaagtggatg gcccagcggg 181 tcaagagtga cagggagtat gcaggactgc ttcaccacat gtccctgcag gacagtgggg 241 gccagagccg ggccatcagc cctgacagcc ccatcagtca gtcctgggct gagatcacca 301 gccaaactga gggcctgagc cgcttgctgc ggcagcacgc agaggatctg aactcagggc 361 ccctgagcaa gctgagcctg ctcatccggg aacggcagca gcttcgcaag acctacagcg 421 agcagtggca gcagctgcag caggagctca ccaagaccca cagccaggac attgagaagc 481 tgaagagcca gtaccgagct ctggcacggg acagtgccca agccaagcgc aagtaccagg 541 aggccagcaa agacaaggac cgtgacaagg ccaaggacaa gtatgtgcgc agcctgtgga 601 agctctttgc tcaccacaac cgctatgtgc tgggcgtgcg ggctgcgcag ctacaccacc 661 agcaccacca ccagctcctg ctgcccggcc tgctgcggtc actgcaggac ctgcacgagg 721 agatggcttg catcctgaag gagatcctgc aggaatacct ggagattagc agcctggtgc 781 aggatgaggt ggtggccatt caccgggaga tggctgcagc tgctgcccgc atccagcctg 841 aggctgagta ccaaggcttc ctgcgacagt atgggtccgc acctgacgtc ccaccctgtg 901 tcacgttcga tgagtcactg cttgaggagg gtgaaccgct ggagcctggg gagctccagc 961 tgaacgagct gactgtggag agcgtgcagc acacgctgac ctcagtgaca gatgagctgg 1021 ctgtggccac cgagatggtg ttcaggcggc aggagatggt tacgcagctg caacaggagc 1081 tccggaatga agaggagaac acccaccccc gggagcgggt gcagctgctg ggcaagaggc 1141 aagtgctgca agaagcactg caggggctgc aggtagcgct gtgcagccag gccaagctgc 1201 aggcccagca ggagttgctg cagaccaagc tggagcacct gggccccggc gagcccccgc 1261 ctgtgctgct cctgcaggat gaccgccact ccacgtcgtc ctcggagcag gagcgagagg 1321 ggggaaggac acccacgctg gagatcctta agagccacat ctcaggaatc ttccgcccca 1381 agttctcgct ccctccaccg ctgcagctca ttccggaggt gcagaagccc ctgcatgagc 1441 agctgtggta ccacggggcc atcccgaggg cagaggtggc tgagctgctg gtgcactctg 1501 gggacttcct ggtgcgggag agccagggca agcaggagta cgtgctgtcg gtgctgtggg 1561 atggtctgcc ccggcacttc atcatccagt ccttggataa cctgtaccga ctggaagggg 1621 aaggctttcc tagcattcct ttgctcatcg accacctact gagcacccag cagcccctca 1681 ccaagaagag tggtgttgtc ctgcacaggg ctgtgcccaa ggacaagtgg gtgctgaacc 1741 atgaggacct ggtgttgggt gagcagattg gacgggggaa ctttggcgaa gtgttcagcg 1801 gacgcctgcg agccgacaac accctggtgg cggtgaagtc ttgtcgagag acgctcccac 1861 ctgacctcaa ggccaagttt ctacaggaag cgaggatcct gaagcagtac agccacccca 1921 acatcgtgcg tctcattggt gtctgcaccc agaagcagcc catctacatc gtcatggagc 1981 ttgtgcaggg gggcgacttc ctgaccttcc tccgcacgga gggggcccgc ctgcgggtga 2041 agactctgct gcagatggtg ggggatgcag ctgctggcat ggagtacctg gagagcaagt 2101 gctgcatcca ccgggacctg gctgctcgga actgcctggt gacagagaag aatgtcctga 2161 agatcagtga ctttgggatg tcccgagagg aagccgatgg ggtctatgca gcctcagggg 2221 gccTCAGACA AGTCCCCGTG AAGTGGACCG CACCTGAGGC CCTTAACTAC GGCCGCTACT 2281 CCTCCGAAAG CGACGTGTGG AGCTTTGGCA TCTTGCTCTG GGAGACCTTC AGCCTGGGGG 2341 CCTCCCCCTA TCCCAACCTC AGCAATCAGC AGACACGGGA GTTTGTGGAG AAGGGGGGCC 2401 GTCTGCCCTG CCCAGAGCTG TGTCCTGATG CCGTGTTCAG GCTCATGGAG CAGTGCTGGG 2461 CCTATGAGCC TGGGCAGCGG CCCAGCTTCA GCACCATCTA CCAGGAGCTG CAGAGCATCC 2521 GAAAGCGGCA TCGGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 2581 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 2641 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACCT AATGATTTCC CATTACAGGG 2701 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t