Transcript: Human XM_017022007.1

PREDICTED: Homo sapiens FES proto-oncogene, tyrosine kinase (FES), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
FES (2242)
Length:
2557
CDS:
81..2339

Additional Resources:

NCBI RefSeq record:
XM_017022007.1
NBCI Gene record:
FES (2242)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001147807 GGCAGACAAGGACCGTGACA pXPR_003 AGG 496 22% 5 0.6731 FES FES 76509
2 BRDN0001147481 CATCGAACGTGACACAGGGT pXPR_003 GGG 827 37% 7 0.1875 FES FES 76508
3 BRDN0001148684 TGAGATCACCAGCCAAACTG pXPR_003 AGG 238 11% 3 0.0008 FES FES 76506
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017022007.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000231680 ATCAGCAGACACGGGAGTTTG pLKO_005 2167 CDS 100% 10.800 15.120 N FES n/a
2 TRCN0000361154 ATCAGCAGACACGGGAGTTTG pLKO_005 2167 CDS 100% 10.800 15.120 N Fes n/a
3 TRCN0000199154 CAACAGGAGCTCCGGAATGAA pLKO.1 1083 CDS 100% 5.625 7.875 N FES n/a
4 TRCN0000199061 CGGCTCCAGCTCATATGCTGA pLKO.1 2397 3UTR 100% 0.000 0.000 N FES n/a
5 TRCN0000255348 TGCAGGAATACCTGGAGATTA pLKO_005 760 CDS 100% 13.200 9.240 N FES n/a
6 TRCN0000380695 ACAAGGCTAAGGACAAGTATG pLKO_005 577 CDS 100% 10.800 7.560 N FES n/a
7 TRCN0000231678 ACCCACGCTGGAGATCCTTAA pLKO_005 1343 CDS 100% 10.800 7.560 N FES n/a
8 TRCN0000231676 CTACTGGAGGGCATGAGAAAG pLKO_005 156 CDS 100% 10.800 7.560 N FES n/a
9 TRCN0000231679 CTGACCTCAAGGCCAAGTTTC pLKO_005 1663 CDS 100% 10.800 7.560 N FES n/a
10 TRCN0000379569 TGGTGTTGGGTGAGCAGATTG pLKO_005 1552 CDS 100% 10.800 7.560 N FES n/a
11 TRCN0000369029 CAGAAGCAGCCCATCTACATC pLKO_005 1752 CDS 100% 4.950 3.465 N FES n/a
12 TRCN0000000627 GAGAAGAATGTCCTGAAGATC pLKO.1 1947 CDS 100% 4.950 3.465 N FES n/a
13 TRCN0000000624 CAAGGCCAAGTTTCTACAGGA pLKO.1 1670 CDS 100% 2.640 1.848 N FES n/a
14 TRCN0000000625 CATTCCTTTGCTCATCGACCA pLKO.1 1436 CDS 100% 2.160 1.512 N FES n/a
15 TRCN0000199245 CTTCTCAAGCTGGTGGCCTCT pLKO.1 2352 3UTR 100% 0.720 0.504 N FES n/a
16 TRCN0000196932 GCTCATATGCTGACAGCTCTT pLKO.1 2405 3UTR 100% 0.000 0.000 N FES n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017022007.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14637 pDONR223 0% 91.4% 91.4% None 504T>C;1320_1321ins210 n/a
2 ccsbBroad304_14637 pLX_304 0% 91.4% 91.4% V5 504T>C;1320_1321ins210 n/a
3 TRCN0000479440 CCTAATGATTTCCCATTACAGGGA pLX_317 12.8% 91.4% 91.4% V5 504T>C;1320_1321ins210;2256delG n/a
4 TRCN0000491795 CTCAGGGGGTATCAGACGTGAACA pLX_317 7.6% 91.4% 91.4% V5 (not translated due to prior stop codon) 15C>T;504T>C;1320_1321ins210 n/a
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