Construct: ORF TRCN0000479541
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010278.1_s317c1
- Derived from:
- ccsbBroadEn_02522
- DNA Barcode:
- CTCCGAGCAGCTAGTGTGTACACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AHCYL1 (10768)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479541
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10768 | AHCYL1 | adenosylhomocysteinase like 1 | NM_006621.7 | 100% | 100% | |
| 2 | human | 10768 | AHCYL1 | adenosylhomocysteinase like 1 | XM_011540535.2 | 93.9% | 92.3% | (many diffs) |
| 3 | human | 10768 | AHCYL1 | adenosylhomocysteinase like 1 | NM_001242673.1 | 91.1% | 91.1% | 0_1ins141 |
| 4 | human | 10768 | AHCYL1 | adenosylhomocysteinase like 1 | NM_001242674.1 | 91.1% | 91.1% | 0_1ins141 |
| 5 | human | 10768 | AHCYL1 | adenosylhomocysteinase like 1 | NM_001242675.2 | 91.1% | 91.1% | 0_1ins141 |
| 6 | human | 10768 | AHCYL1 | adenosylhomocysteinase like 1 | NM_001242676.2 | 91.1% | 91.1% | 0_1ins141 |
| 7 | human | 23382 | AHCYL2 | adenosylhomocysteinase like 2 | XM_017011905.2 | 60.2% | 71.3% | (many diffs) |
| 8 | human | 10768 | AHCYL1 | adenosylhomocysteinase like 1 | XR_001736934.2 | 41.9% | (many diffs) | |
| 9 | human | 10768 | AHCYL1 | adenosylhomocysteinase like 1 | XR_946527.2 | 41.4% | 1_2delGA;1318_1493del;1769_3833del | |
| 10 | human | 10768 | AHCYL1 | adenosylhomocysteinase like 1 | XR_001736935.1 | 36.1% | (many diffs) | |
| 11 | mouse | 229709 | Ahcyl1 | S-adenosylhomocysteine hydr... | NM_145542.3 | 93.6% | 100% | (many diffs) |
| 12 | mouse | 229709 | Ahcyl1 | S-adenosylhomocysteine hydr... | XM_006501408.3 | 85% | 91.1% | (many diffs) |
| 13 | mouse | 229709 | Ahcyl1 | S-adenosylhomocysteine hydr... | XM_017319556.1 | 54.9% | 59% | (many diffs) |
| 14 | mouse | 229709 | Ahcyl1 | S-adenosylhomocysteine hydr... | XM_017319557.1 | 54.9% | 59% | (many diffs) |
| 15 | mouse | 229709 | Ahcyl1 | S-adenosylhomocysteine hydr... | XM_017319558.1 | 50.2% | 51.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1659
- ORF length:
- 1590
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcgatgcct gacgcgatgc cgctgcccgg ggtcggggag gagctgaagc 121 aggccaagga gatcgaggac gccgagaagt actccttcat ggccaccgtc accaaggcgc 181 ccaagaagca aatccagttt gctgatgaca tgcaggagtt caccaaattc cccaccaaaa 241 ctggccgaag atctttgtct cgctcgatct cacagtcctc cactgacagc tacagttcag 301 ctgcatccta cacagatagc tctgatgatg aggtttctcc ccgagagaag cagcaaacca 361 actccaaggg cagcagcaat ttctgtgtga agaacatcaa gcaggcagaa tttggacgcc 421 gggagattga gattgcagag caagacatgt ctgctctgat ttcactcagg aaacgtgctc 481 agggggagaa gcccttggct ggtgctaaaa tagtgggctg tacacacatc acagcccaga 541 cagcggtgtt gattgagaca ctctgtgccc tgggggctca gtgccgctgg tctgcttgta 601 acatctactc aactcagaat gaagtagctg cagcactggc tgaggctgga gttgcagtgt 661 tcgcttggaa gggcgagtca gaagatgact tctggtggtg tattgaccgc tgtgtgaaca 721 tggatgggtg gcaggccaac atgatcctgg atgatggggg agacttaacc cactgggttt 781 ataagaagta tccaaacgtg tttaagaaga tccgaggcat tgtggaagag agcgtgactg 841 gtgttcacag gctgtatcag ctctccaaag ctgggaagct ctgtgttccg gccatgaacg 901 tcaatgattc tgttaccaaa cagaagtttg ataacttgta ctgctgccga gaatccattt 961 tggatggcct gaagaggacc acagatgtga tgtttggtgg gaaacaagtg gtggtgtgtg 1021 gctatggtga ggtaggcaag ggctgctgtg ctgctctcaa agctcttgga gcaattgtct 1081 acattaccga aatcgacccc atctgtgctc tgcaggcctg catggatggg ttcagggtgg 1141 taaagctaaa tgaagtcatc cggcaagtcg atgtcgtaat aacttgcaca ggaaataaga 1201 atgtagtgac acgggagcac ttggatcgca tgaaaaacag ttgtatcgta tgcaatatgg 1261 gccactccaa cacagaaatc gatgtgacca gccTCCGCAC TCCGGAGCTG ACGTGGGAGC 1321 GAGTACGTTC TCAGGTGGAC CATGTCATCT GGCCAGATGG CAAACGAGTT GTCCTCCTGG 1381 CAGAGGGTCG TCTACTCAAT TTGAGCTGCT CCACAGTTCC CACCTTTGTT CTGTCCATCA 1441 CAGCCACAAC ACAGGCTTTG GCACTGATAG AACTCTATAA TGCACCCGAG GGGCGATACA 1501 AGCAGGATGT GTACTTGCTT CCTAAGAAAA TGGATGAATA CGTTGCCAGC TTGCATCTGC 1561 CATCATTTGA TGCCCACCTT ACAGAGCTGA CAGATGACCA AGCAAAATAT CTGGGACTCA 1621 ACAAAAATGG GCCATTCAAA CCTAATTATT ACAGATACTT GCCAACTTTC TTGTACAAAG 1681 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1741 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1801 ACGACTCCGA GCAGCTAGTG TGTACACCAC GCGTTAAGTC gacaatcaac ctctggatta 1861 caaaatttgt gaaagatt