Construct: ORF TRCN0000479541

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010278.1_s317c1
Derived from:
ccsbBroadEn_02522
DNA Barcode:
CTCCGAGCAGCTAGTGTGTACACC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
AHCYL1 (10768)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000479541
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10768 AHCYL1 adenosylhomocysteinase like 1 NM_006621.7 100% 100%
2 human 10768 AHCYL1 adenosylhomocysteinase like 1 XM_011540535.2 93.9% 92.3% (many diffs)
3 human 10768 AHCYL1 adenosylhomocysteinase like 1 NM_001242673.1 91.1% 91.1% 0_1ins141
4 human 10768 AHCYL1 adenosylhomocysteinase like 1 NM_001242674.1 91.1% 91.1% 0_1ins141
5 human 10768 AHCYL1 adenosylhomocysteinase like 1 NM_001242675.2 91.1% 91.1% 0_1ins141
6 human 10768 AHCYL1 adenosylhomocysteinase like 1 NM_001242676.2 91.1% 91.1% 0_1ins141
7 human 23382 AHCYL2 adenosylhomocysteinase like 2 XM_017011905.2 60.2% 71.3% (many diffs)
8 human 10768 AHCYL1 adenosylhomocysteinase like 1 XR_001736934.2 41.9% (many diffs)
9 human 10768 AHCYL1 adenosylhomocysteinase like 1 XR_946527.2 41.4% 1_2delGA;1318_1493del;1769_3833del
10 human 10768 AHCYL1 adenosylhomocysteinase like 1 XR_001736935.1 36.1% (many diffs)
11 mouse 229709 Ahcyl1 S-adenosylhomocysteine hydr... NM_145542.3 93.6% 100% (many diffs)
12 mouse 229709 Ahcyl1 S-adenosylhomocysteine hydr... XM_006501408.3 85% 91.1% (many diffs)
13 mouse 229709 Ahcyl1 S-adenosylhomocysteine hydr... XM_017319556.1 54.9% 59% (many diffs)
14 mouse 229709 Ahcyl1 S-adenosylhomocysteine hydr... XM_017319557.1 54.9% 59% (many diffs)
15 mouse 229709 Ahcyl1 S-adenosylhomocysteine hydr... XM_017319558.1 50.2% 51.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1659
ORF length:
1590
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gtcgatgcct gacgcgatgc cgctgcccgg ggtcggggag gagctgaagc
121aggccaagga gatcgaggac gccgagaagt actccttcat ggccaccgtc accaaggcgc
181ccaagaagca aatccagttt gctgatgaca tgcaggagtt caccaaattc cccaccaaaa
241ctggccgaag atctttgtct cgctcgatct cacagtcctc cactgacagc tacagttcag
301ctgcatccta cacagatagc tctgatgatg aggtttctcc ccgagagaag cagcaaacca
361actccaaggg cagcagcaat ttctgtgtga agaacatcaa gcaggcagaa tttggacgcc
421gggagattga gattgcagag caagacatgt ctgctctgat ttcactcagg aaacgtgctc
481agggggagaa gcccttggct ggtgctaaaa tagtgggctg tacacacatc acagcccaga
541cagcggtgtt gattgagaca ctctgtgccc tgggggctca gtgccgctgg tctgcttgta
601acatctactc aactcagaat gaagtagctg cagcactggc tgaggctgga gttgcagtgt
661tcgcttggaa gggcgagtca gaagatgact tctggtggtg tattgaccgc tgtgtgaaca
721tggatgggtg gcaggccaac atgatcctgg atgatggggg agacttaacc cactgggttt
781ataagaagta tccaaacgtg tttaagaaga tccgaggcat tgtggaagag agcgtgactg
841gtgttcacag gctgtatcag ctctccaaag ctgggaagct ctgtgttccg gccatgaacg
901tcaatgattc tgttaccaaa cagaagtttg ataacttgta ctgctgccga gaatccattt
961tggatggcct gaagaggacc acagatgtga tgtttggtgg gaaacaagtg gtggtgtgtg
1021gctatggtga ggtaggcaag ggctgctgtg ctgctctcaa agctcttgga gcaattgtct
1081acattaccga aatcgacccc atctgtgctc tgcaggcctg catggatggg ttcagggtgg
1141taaagctaaa tgaagtcatc cggcaagtcg atgtcgtaat aacttgcaca ggaaataaga
1201atgtagtgac acgggagcac ttggatcgca tgaaaaacag ttgtatcgta tgcaatatgg
1261gccactccaa cacagaaatc gatgtgacca gccTCCGCAC TCCGGAGCTG ACGTGGGAGC
1321GAGTACGTTC TCAGGTGGAC CATGTCATCT GGCCAGATGG CAAACGAGTT GTCCTCCTGG
1381CAGAGGGTCG TCTACTCAAT TTGAGCTGCT CCACAGTTCC CACCTTTGTT CTGTCCATCA
1441CAGCCACAAC ACAGGCTTTG GCACTGATAG AACTCTATAA TGCACCCGAG GGGCGATACA
1501AGCAGGATGT GTACTTGCTT CCTAAGAAAA TGGATGAATA CGTTGCCAGC TTGCATCTGC
1561CATCATTTGA TGCCCACCTT ACAGAGCTGA CAGATGACCA AGCAAAATAT CTGGGACTCA
1621ACAAAAATGG GCCATTCAAA CCTAATTATT ACAGATACTT GCCAACTTTC TTGTACAAAG
1681TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT
1741GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG
1801ACGACTCCGA GCAGCTAGTG TGTACACCAC GCGTTAAGTC gacaatcaac ctctggatta
1861caaaatttgt gaaagatt

Download FASTA (ORF) (Full)