Construct: ORF TRCN0000479571
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001052.1_s317c1
- Derived from:
- ccsbBroadEn_11660
- DNA Barcode:
- GGACAGTTGTGGCATGATTTTGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RBM34 (23029)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479571
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23029 | RBM34 | RNA binding motif protein 34 | NM_015014.4 | 98.8% | 98.8% | 1_15del |
| 2 | human | 23029 | RBM34 | RNA binding motif protein 34 | NM_001346738.2 | 98.6% | 98.3% | 1_15del;889_890insAAG |
| 3 | human | 23029 | RBM34 | RNA binding motif protein 34 | XM_011544133.2 | 94.1% | 94.1% | 1_15del;596_597ins60 |
| 4 | human | 23029 | RBM34 | RNA binding motif protein 34 | NR_027762.3 | 61.6% | 1_39del;252_253ins137;1178_1710del | |
| 5 | human | 23029 | RBM34 | RNA binding motif protein 34 | NR_144491.2 | 61.4% | (many diffs) | |
| 6 | human | 23029 | RBM34 | RNA binding motif protein 34 | NR_144490.2 | 60.9% | 1_39del;253_497del;1560_2092del | |
| 7 | human | 23029 | RBM34 | RNA binding motif protein 34 | NR_144492.2 | 58% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1341
- ORF length:
- 1275
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag caaacggaag agaaagagaa gtgtccagga gggagagaat cctgacgacg 121 gcgttcgcgg gagtccgccg gaagactaca ggcttggaca ggtcgccagt agcttatttc 181 gcggcgaaca ccattccaga ggtggcaccg gtcggctggc gtccctcttc agttctctgg 241 agccccagat tcaacccgtg tacgtgcctg tgcctaaaca aaccatcaaa aaaacgaaac 301 ggaatgagga ggaagaaagt acatcccaga ttgaaagacc actttcgcaa gaacctgcca 361 aaaaagtgaa agcgaagaag aaacacacta acgcagaaaa aaagttggca gacagggaaa 421 gcgctctagc gagtgctgat ttagaagaag aaattcacca gaaacaaggg cagaaaagga 481 aaaattctca acctggtgtt aaagtagcag atagaaaaat acttgatgac acagaagaca 541 cagttgtcag tcaaagaaag aaaattcaaa tcaaccaaga agaagagaga ttaaagaatg 601 agagaactgt gtttgttggg aatttgcctg ttacatgtaa taagaagaag ctgaagtcgt 661 tttttaaaga gtatggacaa atagaatctg tacgatttcg ttctctgatt ccagcagagg 721 gaacgctatc caaaaagttg gcagcaataa aacgtaaaat tcatcctgat cagaaaaata 781 ttaatgccta tgttgtgttt aaggaggaga gtgctgccac gcaagcattg aaaagaaatg 841 gggcccagat tgcagatgga tttcgtatta gagttgatct cgcatctgag acctcatcta 901 gagacaagag atcggttttt gtggggaatc tcccttataa agttgaagaa tctgccattg 961 agaagcactt tctggactgt ggaagtatca tggccgtgag gattgtgaga gacaaaatga 1021 caggcatcgg caaagggttt GGCTATGTGC TCTTTGAGAA TACAGATTCT GTTCATCTTG 1081 CTCTGAAATT AAATAATTCT GAACTCATGG GGAGAAAACT CAGAGTCATG CGTTCTGTTA 1141 ATAAAGAAAA ATTTAAACAA CAAAATTCAA ATCCACGATT GAAGAATGTC AGTAAACCTA 1201 AGCAGGGACT TAATTTTACT TCCAAAACTG CAGAAGGACA TCCTAAAAGC TTATTTATTG 1261 GAGAAAAAGC TGTTCTCCTT AAAACGAAGA AGAAAGGACA GAAGAAAAGT GGACGCCCTA 1321 AGAAACAGAG AAAACAGAAA TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1381 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1441 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGGAC AGTTGTGGCA 1501 TGATTTTGTA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt