Transcript: Human NR_144491.2

Homo sapiens RNA binding motif protein 34 (RBM34), transcript variant 6, non-coding RNA.

Source:
NCBI, updated 2019-07-31
Taxon:
Homo sapiens (human)
Gene:
RBM34 (23029)
Length:
1707
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_144491.2
NBCI Gene record:
RBM34 (23029)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_144491.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000336819 CAGGTCGCCAGTAGCTTATTT pLKO_005 133 3UTR 100% 15.000 21.000 N RBM34 n/a
2 TRCN0000147405 GTGCTCTTTGAGAATACAGAT pLKO.1 881 3UTR 100% 4.950 6.930 N RBM34 n/a
3 TRCN0000336816 GTGCTCTTTGAGAATACAGAT pLKO_005 881 3UTR 100% 4.950 6.930 N RBM34 n/a
4 TRCN0000150268 GTATTAGAGTTGATCTCGCAT pLKO.1 702 3UTR 100% 2.640 3.696 N RBM34 n/a
5 TRCN0000412959 GTCATGCGTTCTGTTAATAAA pLKO_005 959 3UTR 100% 15.000 10.500 N Rbm34 n/a
6 TRCN0000147509 GCTATGTGCTCTTTGAGAATA pLKO.1 876 3UTR 100% 13.200 9.240 N RBM34 n/a
7 TRCN0000336818 TGCAGATGGATTTCGTATTAG pLKO_005 688 3UTR 100% 13.200 9.240 N RBM34 n/a
8 TRCN0000147133 CACGATTGAAGAATGTCAGTA pLKO.1 1008 3UTR 100% 4.950 3.465 N RBM34 n/a
9 TRCN0000336817 CACGATTGAAGAATGTCAGTA pLKO_005 1008 3UTR 100% 4.950 3.465 N RBM34 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_144491.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11660 pDONR223 100% 61.4% None (many diffs) n/a
2 ccsbBroad304_11660 pLX_304 0% 61.4% V5 (many diffs) n/a
3 TRCN0000479571 GGACAGTTGTGGCATGATTTTGTA pLX_317 24% 61.4% V5 (many diffs) n/a
4 ccsbBroadEn_13781 pDONR223 100% 14.3% None (many diffs) n/a
5 ccsbBroad304_13781 pLX_304 0% 14.3% V5 (many diffs) n/a
6 TRCN0000469746 TCCCTGCGCCGTCCGGGTTTTCGA pLX_317 100% 14.3% V5 (many diffs) n/a
7 ccsbBroadEn_10792 pDONR223 100% 12.6% None (many diffs) n/a
8 ccsbBroad304_10792 pLX_304 0% 12.6% V5 (many diffs) n/a
9 TRCN0000472287 GCCTGGAGAGCTTTCCTCGTCACG pLX_317 100% 12.6% V5 (many diffs) n/a
10 ccsbBroadEn_12783 pDONR223 100% 11.2% None (many diffs) n/a
11 ccsbBroad304_12783 pLX_304 0% 11.2% V5 (many diffs) n/a
12 TRCN0000478282 TATCTGCTCCACCGGGCTCCGTTG pLX_317 100% 11.2% V5 (many diffs) n/a
13 ccsbBroadEn_11616 pDONR223 100% 10.3% None (many diffs) n/a
14 ccsbBroad304_11616 pLX_304 0% 10.3% V5 (many diffs) n/a
15 TRCN0000467678 CCTCCCCTCACACCTCGTCAAAAC pLX_317 100% 10.3% V5 (many diffs) n/a
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