Construct: ORF TRCN0000479573
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016121.1_s317c1
- Derived from:
- ccsbBroadEn_15207
- DNA Barcode:
- TCAACGTGAACCTTCGTTCCTCGT
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF439 (90594)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479573
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 284390 | ZNF763 | zinc finger protein 763 | NM_001367174.2 | 72.8% | 57.3% | (many diffs) |
| 2 | human | 90594 | ZNF439 | zinc finger protein 439 | NM_001348724.1 | 59.9% | 60% | 654_1089del |
| 3 | human | 90594 | ZNF439 | zinc finger protein 439 | NM_001348725.1 | 59.9% | 60% | 654_1089del |
| 4 | human | 284390 | ZNF763 | zinc finger protein 763 | NM_001367172.2 | 50.3% | 39.5% | (many diffs) |
| 5 | human | 284390 | ZNF763 | zinc finger protein 763 | NM_001367173.2 | 50.1% | 39.3% | (many diffs) |
| 6 | human | 284390 | ZNF763 | zinc finger protein 763 | NM_001012753.2 | 50% | 39.2% | (many diffs) |
| 7 | human | 90594 | ZNF439 | zinc finger protein 439 | NM_001348722.1 | 48.1% | 48.2% | 1_267del;921_1356del |
| 8 | human | 90594 | ZNF439 | zinc finger protein 439 | NM_001348723.1 | 48.1% | 48.2% | 1_267del;921_1356del |
| 9 | human | 90594 | ZNF439 | zinc finger protein 439 | NM_001348720.1 | 44.7% | 44.8% | 1_369del;1023_1458del |
| 10 | human | 90594 | ZNF439 | zinc finger protein 439 | NM_001348721.1 | 44.7% | 44.8% | 1_369del;1023_1458del |
| 11 | human | 90594 | ZNF439 | zinc finger protein 439 | NM_152262.2 | 43.6% | 43.6% | 1_408del;1062_1497del |
| 12 | human | 90594 | ZNF439 | zinc finger protein 439 | NM_001348719.1 | 43.1% | 43.2% | 1_423del;1077_1512del |
| 13 | human | 126070 | ZNF440 | zinc finger protein 440 | XM_017026254.1 | 43.1% | 39.7% | (many diffs) |
| 14 | human | 90594 | ZNF439 | zinc finger protein 439 | NM_001348718.1 | 42.9% | 42.9% | 1_432del;1086_1521del |
| 15 | human | 7620 | ZNF69 | zinc finger protein 69 | NM_001364731.1 | 36.9% | 34.4% | (many diffs) |
| 16 | human | 7620 | ZNF69 | zinc finger protein 69 | NM_001364730.1 | 36% | 33.5% | (many diffs) |
| 17 | human | 7620 | ZNF69 | zinc finger protein 69 | XM_006722873.4 | 35.8% | 33.3% | (many diffs) |
| 18 | human | 126070 | ZNF440 | zinc finger protein 440 | NM_152357.3 | 34.2% | 31.5% | (many diffs) |
| 19 | human | 126070 | ZNF440 | zinc finger protein 440 | XM_005259731.4 | 34.1% | 31.4% | (many diffs) |
| 20 | human | 90592 | ZNF700 | zinc finger protein 700 | NM_144566.3 | 26.3% | 23.7% | (many diffs) |
| 21 | human | 90592 | ZNF700 | zinc finger protein 700 | NM_001271848.1 | 26.2% | 23.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 720
- ORF length:
- 654
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa catcagaggt gacactggac acaaggcatg tgaatgtcag gaatatggac 121 caaagccatg gaagagtcaa caacctaaaa aagccttcag atatcacccc tccttgagaa 181 cacaagaaag ggatcacact ggaaagaaac cctatgcttg taaagaatgt ggaaaaaaca 241 ttatttacca ttcaagcatt caaagacaca tggtagtgca cagtggggat ggaccttata 301 aatgtaagtt ttgtgggaaa gcattccatt gtctcagttt atatcttatc catgaaagaa 361 ctcacactGG AGAGAAACCG TATGAATGTA AACAATGTGG TAAATCTTTT AGTTATTCTG 421 CTACCCATCG AATACATGAA AGAACTCACA TTGGAGAAAA GCCTTATGAA TGTCAGGAAT 481 GTGGGAAAGC ATTCCATAGT CCCAGATCCT GTCACAGACA TGAAAGGAGT CACATGGGAG 541 AGAAGGCTTA TCAATGTAAG GAATGTGGAA AAGCATTCAT GTGTCCCCGT TATGTTCGTA 601 GACATGAAAG GACCCACTCT AGGAAAAAAC TTTATGAATG TAAGCAGTGT GGGAAAGCAT 661 TATCCTCTCT TACAAGTTTT CAAACACACA TAAGAATGCA CTCTGGAGAA AGACCTTATA 721 CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG 781 TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT 841 TTATATATCT TGTGGAAAGG ACGATCAACG TGAACCTTCG TTCCTCGTAC GCGTTAAGTC 901 gacaatcaac ctctggatta caaaatttgt gaaagatt