Construct: ORF TRCN0000479680
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006743.2_s317c1
- Derived from:
- ccsbBroadEn_07972
- DNA Barcode:
- TCCGGCCTACTACTCTTCTAATAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHMP2B (25978)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479680
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25978 | CHMP2B | charged multivesicular body... | NM_014043.4 | 99.8% | 100% | 312T>C |
| 2 | human | 25978 | CHMP2B | charged multivesicular body... | XM_011533576.2 | 91.8% | 88.6% | (many diffs) |
| 3 | human | 25978 | CHMP2B | charged multivesicular body... | NM_001244644.2 | 80.4% | 80.2% | 1_1delAins124;189T>C |
| 4 | mouse | 68942 | Chmp2b | charged multivesicular body... | NM_026879.2 | 89.6% | 99.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 705
- ORF length:
- 639
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gtccctcttc aagaagaaaa ccgtggatga tgtaataaag gaacagaatc 121 gagagttacg aggtacacag agggctataa tcagagatcg agcagcttta gagaaacaag 181 aaaaacagct ggaattagaa attaagaaaa tggccaagat tggtaataag gaagcttgca 241 aagttttagc caaacaactt gtgcatctac ggaaacagaa gacgagaact ttTGCTGTAA 301 GTTCAAAAGT TACTTCTATG TCTACACAAA CAAAAGTGAT GAATTCCCAA ATGAAGATGG 361 CTGGAGCAAT GTCTACCACA GCAAAAACAA TGCAGGCAGT TAACAAGAAG ATGGATCCAC 421 AAAAGACATT ACAAACAATG CAGAATTTCC AGAAGGAAAA CATGAAAATG GAAATGACTG 481 AAGAAATGAT CAATGATACA CTTGATGACA TCTTTGACGG TTCTGATGAC GAAGAAGAAA 541 GCCAGGATAT TGTGAATCAA GTTCTTGATG AAATTGGAAT TGAAATTTCT GGAAAGATGG 601 CCAAAGCTCC ATCAGCTGCT CGAAGCTTAC CATCTGCCTC TACTTCAAAG GCTACAATCT 661 CAGATGAAGA GATTGAACGG CAACTCAAGG CTTTAGGAGT AGATTACCCA ACTTTCTTGT 721 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 781 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 841 GAAAGGACGA TCCGGCCTAC TACTCTTCTA ATACACGCGT TAAGTCgaca atcaacctct 901 ggattacaaa atttgtgaaa gatt