Construct: ORF TRCN0000479705
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007040.1_s317c1
- Derived from:
- ccsbBroadEn_00004
- DNA Barcode:
- CATTGCATGACGAAACGACATTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACAA1 (30)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479705
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 30 | ACAA1 | acetyl-CoA acyltransferase 1 | NM_001607.4 | 100% | 100% | |
| 2 | human | 30 | ACAA1 | acetyl-CoA acyltransferase 1 | XM_006713122.1 | 85.8% | 85.8% | 816_817ins180 |
| 3 | human | 30 | ACAA1 | acetyl-CoA acyltransferase 1 | NM_001130410.1 | 78% | 78% | 444_445ins99;717_718ins180 |
| 4 | human | 30 | ACAA1 | acetyl-CoA acyltransferase 1 | XM_011533650.2 | 65.8% | 65.8% | 0_1ins435 |
| 5 | human | 30 | ACAA1 | acetyl-CoA acyltransferase 1 | XM_006713123.2 | 64.1% | 64.1% | 0_1ins456 |
| 6 | human | 30 | ACAA1 | acetyl-CoA acyltransferase 1 | NR_024024.1 | 61.2% | (many diffs) | |
| 7 | mouse | 235674 | Acaa1b | acetyl-Coenzyme A acyltrans... | NM_146230.3 | 85.5% | 85.8% | (many diffs) |
| 8 | mouse | 113868 | Acaa1a | acetyl-Coenzyme A acyltrans... | NM_130864.3 | 85.5% | 85.6% | (many diffs) |
| 9 | mouse | 113868 | Acaa1a | acetyl-Coenzyme A acyltrans... | XM_006511923.1 | 85.5% | 85.6% | (many diffs) |
| 10 | mouse | 235674 | Acaa1b | acetyl-Coenzyme A acyltrans... | XM_011242970.1 | 73.2% | 71.8% | (many diffs) |
| 11 | mouse | 235674 | Acaa1b | acetyl-Coenzyme A acyltrans... | XM_006512114.1 | 56% | 55.8% | (many diffs) |
| 12 | mouse | 113868 | Acaa1a | acetyl-Coenzyme A acyltrans... | XM_006511924.3 | 55.6% | 56.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1338
- ORF length:
- 1272
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca gaggctgcag gtagtgctgg gccacctgag gggtccggcc gattccggct 121 ggatgccgca ggccgcgcct tgcctgagcg gtgccccgca ggcctcggcc gcggacgtgg 181 tggtggtgca cgggcggcgc acggccatct gccgggcggg ccgcggcggc ttcaaggaca 241 ccacccccga cgagcttctc tcggcagtca tgaccgcggt tctcaaggac gtgaatctga 301 ggccggaaca gctgggggac atctgtgtcg gaaatgtgct gcagcctggg gccggggcaa 361 tcatggcccg aatcgcccag tttctgagtg acatcccgga gactgtgcct ttgtccactg 421 tcaatagaca gtgttcgtcg gggctacagg cagtggccag catagcaggt ggcatcagaa 481 atgggtctta tgacattggc atggcctgtg gggtggagtc catgtccctg gctgacagag 541 ggaaccctgg aaatattact tcgcgcttga tggagaagga gaaggccaga gattgcctga 601 ttcctatggg gataacctct gagaatgtgg ctgagcggtt tggcatttca cgggagaagc 661 aggatacctt tgccctggct tcccagcaga aggcagcaag agcccagagc aagggctgtt 721 tccaagctga gattgtgcct gtgaccacca cggtccatga tgacaagggc accaagagga 781 gcatcactgt gacccaggat gagggtatcc gccccagcac caccatggag ggcctggcca 841 aactgaagcc tgccttcaag aaagatggtt ctaccacagc tggaaactct agccaggtga 901 gtgatggggc agctgccatc ctgctggccc ggaggtccaa ggcagaagag ttgggccttc 961 ccatccttgg ggtcctgagg tcttatgcag tggttggggt cccacctgac atcatgggca 1021 ttggacctgc cTATGCCATC CCAGTAGCTT TGCAAAAAGC AGGGCTGACA GTGAGTGACG 1081 TGGACATCTT CGAGATCAAT GAGGCCTTTG CAAGCCAGGC TGCCTACTGT GTGGAGAAGC 1141 TACGACTCCC CCCTGAGAAG GTGAACCCCC TGGGGGGTGC AGTGGCCTTA GGGCACCCAC 1201 TGGGCTGCAC TGGGGCACGA CAGGTCATCA CGCTGCTCAA TGAGCTGAAG CGCCGTGGGA 1261 AGAGGGCATA CGGAGTGGTG TCCATGTGCA TCGGGACTGG AATGGGAGCC GCTGCCGTCT 1321 TTGAATACCC TGGGAACTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1381 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1441 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACATTGCA TGACGAAACG 1501 ACATTCCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt