Construct: ORF TRCN0000479742
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009106.1_s317c1
- Derived from:
- ccsbBroadEn_03889
- DNA Barcode:
- GTTCGGCCGCCCCACCAAATAGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- APOBEC3G (60489)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479742
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 60489 | APOBEC3G | apolipoprotein B mRNA editi... | NM_021822.4 | 100% | 100% | |
| 2 | human | 60489 | APOBEC3G | apolipoprotein B mRNA editi... | NM_001349436.1 | 97.1% | 97.1% | 0_1ins33 |
| 3 | human | 60489 | APOBEC3G | apolipoprotein B mRNA editi... | NM_001349437.1 | 82.5% | 82.5% | 0_1ins201 |
| 4 | human | 60489 | APOBEC3G | apolipoprotein B mRNA editi... | XM_017028903.1 | 72.6% | 72.5% | (many diffs) |
| 5 | human | 60489 | APOBEC3G | apolipoprotein B mRNA editi... | XM_017028904.1 | 59.8% | 59.7% | (many diffs) |
| 6 | human | 60489 | APOBEC3G | apolipoprotein B mRNA editi... | NM_001349438.2 | 50.4% | 50.5% | 582_582delGins571 |
| 7 | human | 200316 | APOBEC3F | apolipoprotein B mRNA editi... | NM_001006666.2 | 23.1% | 16.6% | (many diffs) |
| 8 | human | 200316 | APOBEC3F | apolipoprotein B mRNA editi... | XM_017028643.2 | 23.1% | 16.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1218
- ORF length:
- 1152
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gcctcacttc agaaacacag tggagcgaat gtatcgagac acattctcct 121 acaactttta taatagaccc atcctttctc gtcggaatac cgtctggctg tgctacgaag 181 tgaaaacaaa gggtccctca aggccccctt tggacgcaaa gatctttcga ggccaggtgt 241 attccgaact taagtaccac ccagagatga gattcttcca ctggttcagc aagtggagga 301 agctgcatcg tgaccaggag tatgaggtca cctggtacat atcctggagc ccctgcacaa 361 agtgtacaag ggatatggcc acgttcctgg ccgaggaccc gaaggttacc ctgaccatct 421 ttgttgcccg cctctactac ttctgggacc cagattacca ggaggcgctt cgcagcctgt 481 gtcagaaaag agacggtccg cgtgccacca tgaagatcat gaattatgac gaatttcagc 541 actgttggag caagttcgtg tacagccaaa gagagctatt tgagccttgg aataatctgc 601 ctaaatatta tatattactg cacatcatgc tgggggagat tctcagacac tcgatggatc 661 cacccacatt cactttcaac tttaacaatg aaccttgggt cagaggacgg catgagactt 721 acctgtgtta tgaggtggag cgcatgcaca atgacacctg ggtcctgctg aaccagcgca 781 ggggctttct atgcaaccag gctccacata aacacggttt ccttgaaggc cgccatgcag 841 agctgtgctt cctggacgtg attccctttt ggaagctgga cctggaccag gactacaggg 901 ttacctgctt caccTCCTGG AGCCCCTGCT TCAGCTGTGC CCAGGAAATG GCTAAATTCA 961 TTTCAAAAAA CAAACACGTG AGCCTGTGCA TCTTCACTGC CCGCATCTAT GATGATCAAG 1021 GAAGATGTCA GGAGGGGCTG CGCACCCTGG CCGAGGCTGG GGCCAAAATT TCAATAATGA 1081 CATACAGTGA ATTTAAGCAC TGCTGGGACA CCTTTGTGGA CCACCAGGGA TGTCCCTTCC 1141 AGCCCTGGGA TGGACTAGAT GAGCACAGCC AAGACCTGAG TGGGAGGCTG CGGGCCATTC 1201 TCCAGAATCA GGAAAACTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1261 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1321 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGTTCGGC CGCCCCACCA 1381 AATAGGTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt