Construct: ORF TRCN0000479881

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001853.1_s317c1
Derived from:
ccsbBroadEn_00214
DNA Barcode:
GCCACTTTGTAGGCGCCATTCGAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CAPN1 (823)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000479881
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 823 CAPN1 calpain 1 NM_001198868.2 100% 100%
2 human 823 CAPN1 calpain 1 NM_001198869.1 100% 100%
3 human 823 CAPN1 calpain 1 NM_005186.4 100% 100%
4 human 823 CAPN1 calpain 1 XM_006718698.2 100% 100%
5 human 823 CAPN1 calpain 1 XM_011545292.1 100% 100%
6 human 823 CAPN1 calpain 1 NR_040008.2 70.1% 1_87del;353_354ins70;2160_2882del
7 mouse 12333 Capn1 calpain 1 NM_001110504.1 85.8% 89.2% (many diffs)
8 mouse 12333 Capn1 calpain 1 NM_007600.3 85.8% 89.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2208
ORF length:
2142
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtc ggaggagatc atcacgccgg tgtactgcac tggggtgtca gcccaagtgc
121agaagcagcg ggccagggag ctgggcctgg gccgccatga gaatgccatc aagtacctgg
181gccaggatta tgagcagctg cgggtgcgat gcctgcagag tgggaccctc ttccgtgatg
241aggccttccc cccggtaccc cagagcctgg gttacaagga cctgggtccc aattcctcca
301agacctatgg catcaagtgg aagcgtccca cggaactgct gtcaaacccc cagttcattg
361tggatggagc tacccgcaca gacatctgcc agggagcact gggggactgc tggctcttgg
421cggccatcgc ctccctcact ctcaacgaca ccctcctgca ccgagtggtt ccgcacggcc
481agagcttcca gaatggctat gccggcatct tccatttcca gctgtggcaa tttggggagt
541gggtggacgt ggtcgtggat gacctgctgc ccatcaagga cgggaagcta gtgttcgtgc
601actctgccga aggcaacgag ttctggagcg ccctgcttga gaaggcctat gccaaggtaa
661atggcagcta cgaggccctg tcagggggca gcacctcaga gggctttgag gacttcacag
721gcggggttac cgagtggtac gagttgcgca aggctcccag tgacctctac cagatcatcc
781tcaaggcgct ggagcggggc tccctgctgg gctgctccat agacatctcc agcgttctag
841acatggaggc catcactttc aagaagttgg tgaagggcca tgcctactct gtgaccgggg
901ccaagcaggt gaactaccga ggccaggtgg tgagcctgat ccggatgcgg aacccctggg
961gcgaggtgga gtggacggga gcctggagcg acagctcctc agagtggaac aacgtggacc
1021catatgaacg ggaccagctc cgggtcaaga tggaggacgg ggagttctgg atgtcattcc
1081gagacttcat gcgggagttc acccgcctgg agatctgcaa cctcacaccc gacgccctca
1141agagccggac catccgcaaa tggaacacca cactctacga aggcacctgg cggcggggga
1201gcaccgcggg gggctgccga aactacccag ccaccttctg ggtgaaccct cagttcaaga
1261tccggctgga tgagacggat gacccggacg actacgggga ccgcgagtca ggctgcagct
1321tcgtgctcgc ccttatgcag aagcaccgtc gccgcgagcg ccgcttcggc cgcgacatgg
1381agactattgg cttcgcggtc tacgaggtcc ctccggagct ggtgggccag ccggccgtac
1441acttgaagcg tgacttcttc ctggccaatg cgtctcgggc gcgctcagag cagttcatca
1501acctgcgaga ggtcagcacc cgcttccgcc tgccacccgg ggagtatgtg gtggtgccct
1561ccaccttcga gcccaacaag gagggcgact tcgtgctgcg cttcttctca gagaagagtg
1621ctgggactgt ggagctggat gaccagatcc aggccaatct ccccgatgag caagtgctct
1681cagaagagga gattgacgag aacttcaagg ccctcttcag gcagctggca ggggaggaca
1741tggagatcag cgtgaaggag ttgcggacaa tcctcaatag gatcatcagc aaacacaaag
1801accTGCGGAC CAAGGGCTTC AGCCTAGAGT CGTGCCGCAG CATGGTGAAC CTCATGGATC
1861GTGATGGCAA TGGGAAGCTG GGCCTGGTGG AGTTCAACAT CCTGTGGAAC CGCATCCGGA
1921ATTACCTGTC CATCTTCCGG AAGTTTGACC TGGACAAGTC GGGCAGCATG AGTGCCTACG
1981AGATGCGGAT GGCCATTGAG TCGGCAGGCT TCAAGCTCAA CAAGAAGCTG TACGAGCTCA
2041TCATCACCCG CTACTCGGAG CCCGACCTGG CGGTCGACTT TGACAATTTC GTTTGCTGCC
2101TGGTGCGGCT AGAGACCATG TTCCGATTTT TCAAAACTCT GGACACAGAT CTGGATGGAG
2161TTGTGACCTT TGACTTGTTT AAGTGGTTGC AGCTGACCAT GTTTGCATGC CCAACTTTCT
2221TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA
2281CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT
2341GTGGAAAGGA CGAGCCACTT TGTAGGCGCC ATTCGACACG CGTTAAGTCg acaatcaacc
2401tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)