Construct: ORF TRCN0000480027
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005300.1_s317c1
- Derived from:
- ccsbBroadEn_03636
- DNA Barcode:
- TGGCAGCCGGTCCTCCTTCGACCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAR2 (55711)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480027
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55711 | FAR2 | fatty acyl-CoA reductase 2 | NM_001271783.2 | 99.9% | 100% | 78G>A |
| 2 | human | 55711 | FAR2 | fatty acyl-CoA reductase 2 | NM_018099.5 | 99.9% | 100% | 78G>A |
| 3 | human | 55711 | FAR2 | fatty acyl-CoA reductase 2 | XM_011520747.2 | 96.1% | 96.2% | 1_60del;138G>A |
| 4 | human | 55711 | FAR2 | fatty acyl-CoA reductase 2 | XM_017019624.2 | 91% | 88.6% | (many diffs) |
| 5 | human | 55711 | FAR2 | fatty acyl-CoA reductase 2 | XM_011520748.3 | 87.6% | 85.4% | (many diffs) |
| 6 | human | 55711 | FAR2 | fatty acyl-CoA reductase 2 | NM_001271784.2 | 81.1% | 81.1% | 0_1ins291 |
| 7 | human | 55711 | FAR2 | fatty acyl-CoA reductase 2 | XM_017019625.2 | 58.8% | 58.8% | 0_1ins636 |
| 8 | mouse | 330450 | Far2 | fatty acyl CoA reductase 2 | NM_001347516.1 | 84.1% | 87.9% | (many diffs) |
| 9 | mouse | 330450 | Far2 | fatty acyl CoA reductase 2 | XM_006507070.3 | 84.1% | 87.9% | (many diffs) |
| 10 | mouse | 330450 | Far2 | fatty acyl CoA reductase 2 | NM_178797.3 | 81% | 84.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1611
- ORF length:
- 1545
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc cacaattgca gctttctatg gcggcaagtc cattctcatc acgggggcca 121 caggctttct gggcaaagtg ctaatggaga agctgtttcg caccagccca gacctgaaag 181 tcatttacat ccttgtgagg cccaaggctg gccagacact gcagcagagg gttttccaga 241 tcctagacag taagctattt gagaaagtca aagaagtttg tccaaatgtg catgagaaga 301 tcagagctat ttatgcagat ctcaatcaga atgactttgc catcagcaaa gaggacatgc 361 aggagcttct ctcctgtaca aacataatat ttcactgtgc agccactgta cgctttgacg 421 acactctcag acatgctgtg caacttaacg tcactgccac ccggcagctc ttgcttatgg 481 ctagtcagat gccaaagctg gaagccttta tacatatctc tactgcctat tcaaattgta 541 acctgaagca catcgatgaa gttatctatc cgtgccctgt ggagccaaaa aaaatcattg 601 attcccttga gtggttagac gatgctatta ttgacgagat tacacccaag ctgatcagag 661 attggcccaa tatttatacc tacaccaagg ccttgggaga aatggtggtg cagcaagaga 721 gcaggaacct gaacattgcc atcataaggc cctccattgt gggagcaact tggcaggagc 781 ctttcccagg ttgggttgat aatataaatg gacctaatgg aatcattatt gcgactggga 841 aagggtttct tcgggccata aaagctactc caatggctgt ggcagacgta attccagttg 901 atacagtcgt caatctcatg ctagctgtag gatggtatac tgcagttcac agacctaagt 961 caacattagt ctaccacatt acatctggta acatgaatcc ctgcaattgg cacaaaatgg 1021 gagtccaagt cttggcaacc tttgaaaaaa tcccatttga gagacctttc aggaggccaa 1081 atgctaattt taccagcaac agcttcacat cacagtactg gaatgcggtc agccaccggg 1141 cccctgccat tatctatgac tgctatctgc ggctcactgg aaggaagccc aggatgacaa 1201 agctcatgaa tcggcttttA AGAACTGTTT CCATGTTGGA GTATTTCATC AACCGGAGTT 1261 GGGAATGGAG CACGTACAAT ACAGAAATGC TGATGTCTGA GCTGAGTCCT GAAGACCAGA 1321 GAGTATTCAA CTTTGACGTG CGCCAGTTGA ACTGGTTGGA ATACATTGAA AATTATGTTT 1381 TGGGAGTTAA AAAATACTTA TTGAAAGAGG ATATGGCTGG GATCCCAAAA GCAAAGCAAC 1441 GCTTAAAAAG GCTCCGAAAT ATTCACTACC TCTTTAATAC TGCCCTCTTC CTTATCGCCT 1501 GGCGCCTTCT CATTGCAAGA TCTCAGATGG CTCGGAATGT CTGGTTCTTC ATTGTAAGCT 1561 TCTGTTATAA ATTCCTCTCC TACTTTAGAG CATCCAGCAC GCTCAAAGTT TACCCAACTT 1621 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1681 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1741 CTTGTGGAAA GGACGATGGC AGCCGGTCCT CCTTCGACCC ACGCGTTAAG TCgacaatca 1801 acctctggat tacaaaattt gtgaaagatt