Construct: ORF TRCN0000480033
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017018.1_s317c1
- Derived from:
- ccsbBroadEn_15857
- DNA Barcode:
- GAATAAAAGGAACACGTGTTTGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MYOZ2 (51778)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480033
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51778 | MYOZ2 | myozenin 2 | NM_016599.5 | 99.8% | 100% | 237A>G |
| 2 | human | 51778 | MYOZ2 | myozenin 2 | XM_006714234.4 | 72.7% | 71.9% | (many diffs) |
| 3 | mouse | 59006 | Myoz2 | myozenin 2 | NM_021503.2 | 86.9% | 88.2% | (many diffs) |
| 4 | mouse | 59006 | Myoz2 | myozenin 2 | XM_006501828.3 | 77.3% | 79.9% | (many diffs) |
| 5 | mouse | 59006 | Myoz2 | myozenin 2 | XM_006501829.3 | 77.3% | 79.9% | (many diffs) |
| 6 | mouse | 59006 | Myoz2 | myozenin 2 | XM_006501830.3 | 59.3% | 60.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 858
- ORF length:
- 792
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct atcacataat actatgatga agcagagaaa acagcaagca acagccatca 121 tgaaggaagt ccatggaaat gatgttgatg gcatggacct gggcaaaaag gtcagcatcc 181 ccagagacat catgttggaa gaattatccc atctcagtaa ccgtggtgcc aggctattta 241 agatgcgtca aagaagatct gacaaataca catttgaaaa tttccagtat caatctagag 301 cgcaaataaa tcacagtatt gctatgcaga atgggaaagt ggatggaagt aacttggaag 361 gtggttcgca gcaagccccc ttgactcctc ccaacacccc agatccacga agccctccaa 421 atccagacaa cattgctcca ggatattctg gaccactgaa ggaaattcct cctgaaaaat 481 tcaacaccac agctgtcccT AAGTACTATC AATCTCCCTG GGAACAAGCC ATTAGCAATG 541 ATCCGGAGCT TTTAGAGGCT TTATATCCTA AACTTTTCAA GCCTGAAGGA AAGGCAGAAC 601 TGCCTGATTA CAGGAGCTTT AACAGGGTTG CCACACCATT TGGAGGTTTT GAAAAAGCAT 661 CAAGAATGGT TAAATTTAAA GTTCCAGATT TTGAGCTACT ATTGCTAACA GATCCCAGGT 721 TTATGTCCTT TGTCAATCCC CTTTCTGGCA GACGGTCCTT TAATAGGACT CCTAAGGGAT 781 GGATATCTGA GAATATTCCT ATAGTGATAA CAACCGAACC TACAGATGAT ACCACTGTAC 841 CAGAATCAGA AGACCTATGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 901 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 961 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGAATAAA AGGAACACGT 1021 GTTTGCGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt