Construct: ORF TRCN0000480232
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017402.1_s317c1
- Derived from:
- ccsbBroadEn_02942
- DNA Barcode:
- CAAACACCTAAATTATAGCACTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FBXO25 (26260)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480232
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26260 | FBXO25 | F-box protein 25 | NM_012173.3 | 100% | 100% | |
| 2 | human | 26260 | FBXO25 | F-box protein 25 | XM_011534749.1 | 97% | 97% | 784_810del |
| 3 | human | 26260 | FBXO25 | F-box protein 25 | NM_183420.2 | 81.2% | 72% | 1_151del;237_286del |
| 4 | human | 26260 | FBXO25 | F-box protein 25 | XM_024447123.1 | 81.2% | 72% | 1_151del;237_286del |
| 5 | human | 26260 | FBXO25 | F-box protein 25 | XM_024447124.1 | 81.2% | 72% | 1_151del;237_286del |
| 6 | human | 26260 | FBXO25 | F-box protein 25 | NM_183421.2 | 79.2% | 70.2% | 1_151del;237_286del;985_1011del |
| 7 | human | 26260 | FBXO25 | F-box protein 25 | XM_011534748.3 | 79.2% | 70.2% | 1_151del;237_286del;985_1011del |
| 8 | human | 26260 | FBXO25 | F-box protein 25 | XM_017013313.2 | 79.2% | 70.2% | 1_151del;237_286del;985_1011del |
| 9 | human | 26260 | FBXO25 | F-box protein 25 | XM_024447125.1 | 78.9% | 67.7% | (many diffs) |
| 10 | human | 26260 | FBXO25 | F-box protein 25 | XM_024447126.1 | 78.9% | 67.7% | (many diffs) |
| 11 | human | 26260 | FBXO25 | F-box protein 25 | XM_017013314.1 | 76.9% | 66.1% | (many diffs) |
| 12 | human | 26260 | FBXO25 | F-box protein 25 | XR_002956627.1 | 13.1% | (many diffs) | |
| 13 | mouse | 66822 | Fbxo25 | F-box protein 25 | NM_025785.2 | 67.8% | 66.6% | (many diffs) |
| 14 | mouse | 66822 | Fbxo25 | F-box protein 25 | XM_006508854.3 | 66.2% | 65% | (many diffs) |
| 15 | mouse | 66822 | Fbxo25 | F-box protein 25 | NM_001347316.1 | 66% | 64.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 939
- ORF length:
- 873
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gaaatattca ataatgaaga gcatgaatat gcatcgaaaa aaaggaaaaa 121 ggaccatttt agaaatgaca caaatactca aaaggcatgg ctattgcacc ttgggagaag 181 cctttaatcg gttagacttc tcaagtgcaa ttcaagatat ccgaaggttc aattatgtgg 241 tcaaactgtt gcagctaatt gcaaaatccc agttaacttc attgagtggc gtggcacaga 301 agaattactt caacattttg gataaaatcg ttcaaaaggt tcttgatgac caccacaatc 361 ctcgcttaat caaagatctt ctgcaagacc taagctctac cctctgcatt cttattagag 421 gagtagggaa gtctgtatta gtgggaaaca tcaatatttg gatttgccga ttagaaacta 481 ttctcgcctg gcaacaacag ctacaggatc ttcagatgac taagcaagtg aacaatggcc 541 tcaccctcag tgaccttcct ctgcacatgc tgaacaacat cctataccgg ttctcagacg 601 gatgggacat caTCACCTTA GGCCAGGTGA CCCCCACGTT GTATATGCTT AGTGAAGACA 661 GACAGCTGTG GAAGAAGCTT TGTCAGTACC ATTTTGCTGA AAAGCAGTTT TGTAGACATT 721 TGATCCTTTC AGAAAAAGGT CATATTGAAT GGAAGTTGAT GTACTTTGCA CTTCAGAAAC 781 ATTACCCAGC GAAGGAGCAG TACGGAGACA CACTGCATTT CTGTCGGCAC TGCAGCATTC 841 TCTTTTGGAA GGACTCAGGA CACCCCTGCA CGGCGGCCGA CCCTGACAGC TGCTTCACGC 901 CTGTGTCTCC GCAGCACTTC ATCGACCTCT TCAAGTTTTA CCCAACTTTC TTGTACAAAG 961 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1021 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1081 ACGACAAACA CCTAAATTAT AGCACTTGAC GCGTTAAGTC gacaatcaac ctctggatta 1141 caaaatttgt gaaagatt