Transcript: Human XM_017013314.1

PREDICTED: Homo sapiens F-box protein 25 (FBXO25), transcript variant X5, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
FBXO25 (26260)
Length:
1523
CDS:
262..1272

Additional Resources:

NCBI RefSeq record:
XM_017013314.1
NBCI Gene record:
FBXO25 (26260)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017013314.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000004320 GTACGGAGACACACTGCATTT pLKO.1 1104 CDS 100% 10.800 15.120 N FBXO25 n/a
2 TRCN0000314573 GTACGGAGACACACTGCATTT pLKO_005 1104 CDS 100% 10.800 15.120 N FBXO25 n/a
3 TRCN0000350374 TTAGTGGGAAACATCAATATT pLKO_005 742 CDS 100% 15.000 10.500 N FBXO25 n/a
4 TRCN0000004318 CCACCACAATCCTCGCTTAAT pLKO.1 654 CDS 100% 13.200 9.240 N FBXO25 n/a
5 TRCN0000314572 CCACCACAATCCTCGCTTAAT pLKO_005 654 CDS 100% 13.200 9.240 N FBXO25 n/a
6 TRCN0000004321 GAAGTCTGTATTAGTGGGAAA pLKO.1 732 CDS 100% 4.050 2.835 N FBXO25 n/a
7 TRCN0000004317 TCAGAAACATTACCCAGCGAA pLKO.1 1077 CDS 100% 2.640 1.848 N FBXO25 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017013314.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02942 pDONR223 100% 76.9% 66.1% None (many diffs) n/a
2 ccsbBroad304_02942 pLX_304 0% 76.9% 66.1% V5 (many diffs) n/a
3 TRCN0000480232 CAAACACCTAAATTATAGCACTTG pLX_317 37.7% 76.9% 66.1% V5 (many diffs) n/a
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