Construct: ORF TRCN0000480263
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015386.1_s317c1
- Derived from:
- ccsbBroadEn_03592
- DNA Barcode:
- ATTAGACCATCAATGAGCTGTCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHST12 (55501)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480263
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55501 | CHST12 | carbohydrate sulfotransfera... | NM_001243794.2 | 100% | 100% | |
| 2 | human | 55501 | CHST12 | carbohydrate sulfotransfera... | NM_001243795.2 | 100% | 100% | |
| 3 | human | 55501 | CHST12 | carbohydrate sulfotransfera... | NM_018641.5 | 100% | 100% | |
| 4 | human | 55501 | CHST12 | carbohydrate sulfotransfera... | XM_011515443.2 | 100% | 100% | |
| 5 | human | 55501 | CHST12 | carbohydrate sulfotransfera... | XM_011515444.2 | 100% | 100% | |
| 6 | human | 55501 | CHST12 | carbohydrate sulfotransfera... | XR_002956463.1 | 32.1% | 1_77del;1320_3859del | |
| 7 | mouse | 59031 | Chst12 | carbohydrate sulfotransfera... | NM_021528.3 | 82.5% | 82.8% | (many diffs) |
| 8 | mouse | 59031 | Chst12 | carbohydrate sulfotransfera... | XM_006504725.2 | 82.5% | 82.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1308
- ORF length:
- 1242
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac caaggcccgg ctgttccggc tgtggctggt gctggggtcg gtgttcatga 121 tcctgctgat catcgtgtac tgggacagcg caggcgccgc gcacttctac ttgcacacgt 181 ccttctctag gccgcacacg gggccgccgc tgcccacgcc cgggccggac agggacaggg 241 agctcacggc cgactccgat gtcgacgagt ttctggacaa gtttctcagt gctggcgtga 301 agcagagcga ccttcccaga aaggagacgg agcagccgcc tgcgccgggg agcatggagg 361 agagcgtgag aggctacgac tggtccccgc gcgacgcccg gcgcagccca gaccagggcc 421 ggcagcaggc ggagcggagg agcgtgctgc ggggcttctg cgccaactcc agcctggcct 481 tccccaccaa ggagcgcgca ttcgacgaca tccccaactc ggagctgagc cacctgatcg 541 tggacgaccg gcacggggcc atctactgct acgtgcccaa ggtggcctgc accaactgga 601 agcgcgtgat gatcgtgctg agcggaagcc tgctgcaccg cggtgcgccc taccgcgacc 661 cgctgcgcat cccgcgcgag cacgtgcaca acgccagcgc gcacctgacc ttcaacaagt 721 tctggcgccg ctacgggaag ctctcccgcc acctcatgaa ggtcaagctc aagaagtaca 781 ccaagttcct cttcgtgcgc gaccccttcg tgcgcctgat ctccgccttc cgcagcaagt 841 tcgagctgga gaacgaggag ttctaccgca agttcgccgt gcccatgctg cggctgtacg 901 ccaaccacac cagcctgccc gcctcggcgc gcgaggcctt ccgcgctggc ctcaaggtgt 961 ccTTCGCCAA CTTCATCCAG TACCTGCTGG ACCCGCACAC GGAGAAGCTG GCGCCCTTCA 1021 ACGAGCACTG GCGGCAGGTG TACCGCCTCT GCCACCCGTG CCAGATCGAC TACGACTTCG 1081 TGGGGAAGCT GGAGACTCTG GACGAGGACG CCGCGCAGCT GCTGCAGCTA CTCCAGGTGG 1141 ACCGGCAGCT CCGCTTCCCC CCGAGCTACC GGAACAGGAC CGCCAGCAGC TGGGAGGAGG 1201 ACTGGTTCGC CAAGATCCCC CTGGCCTGGA GGCAGCAGCT GTATAAACTC TACGAGGCCG 1261 ACTTTGTTCT CTTCGGCTAC CCCAAGCCCG AAAACCTCCT CCGAGACTGC CCAACTTTCT 1321 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1381 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1441 GTGGAAAGGA CGAATTAGAC CATCAATGAG CTGTCCTACG CGTTAAGTCg acaatcaacc 1501 tctggattac aaaatttgtg aaagatt