Construct: ORF TRCN0000480355
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006384.2_s317c1
- Derived from:
- ccsbBroadEn_03054
- DNA Barcode:
- CTTCTCTTCATGCTTACGGGAGCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IFT81 (28981)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480355
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 28981 | IFT81 | intraflagellar transport 81 | NM_001347946.2 | 100% | 100% | |
| 2 | human | 28981 | IFT81 | intraflagellar transport 81 | NM_031473.4 | 100% | 100% | |
| 3 | human | 28981 | IFT81 | intraflagellar transport 81 | XR_944523.3 | 74.2% | 1_112del;1406_1741del | |
| 4 | human | 28981 | IFT81 | intraflagellar transport 81 | NM_001143779.2 | 61.1% | 57.3% | (many diffs) |
| 5 | human | 28981 | IFT81 | intraflagellar transport 81 | NM_014055.4 | 61.1% | 57.3% | (many diffs) |
| 6 | human | 28981 | IFT81 | intraflagellar transport 81 | XM_017019217.1 | 61.1% | 57.3% | (many diffs) |
| 7 | human | 28981 | IFT81 | intraflagellar transport 81 | XR_001748670.2 | 59.3% | 1_548del;1842_2177del | |
| 8 | human | 28981 | IFT81 | intraflagellar transport 81 | XR_429093.3 | 45.8% | (many diffs) | |
| 9 | human | 28981 | IFT81 | intraflagellar transport 81 | NR_144951.2 | 43.9% | (many diffs) | |
| 10 | human | 28981 | IFT81 | intraflagellar transport 81 | XR_001748672.2 | 40.3% | 1_112del;1150_1271del;1528_3203del | |
| 11 | human | 28981 | IFT81 | intraflagellar transport 81 | NR_144952.2 | 38.3% | (many diffs) | |
| 12 | human | 28981 | IFT81 | intraflagellar transport 81 | NR_144950.2 | 38.2% | (many diffs) | |
| 13 | human | 28981 | IFT81 | intraflagellar transport 81 | NR_144949.2 | 35.5% | (many diffs) | |
| 14 | mouse | 12589 | Ift81 | intraflagellar transport 81 | XM_006530128.2 | 59.5% | 58.7% | (many diffs) |
| 15 | mouse | 12589 | Ift81 | intraflagellar transport 81 | XM_011248162.1 | 54.4% | 53.8% | (many diffs) |
| 16 | mouse | 12589 | Ift81 | intraflagellar transport 81 | NM_009879.3 | 54.3% | 53.7% | (many diffs) |
| 17 | mouse | 12589 | Ift81 | intraflagellar transport 81 | XM_006530127.3 | 54.3% | 53.7% | (many diffs) |
| 18 | mouse | 12589 | Ift81 | intraflagellar transport 81 | XM_006530129.3 | 44.9% | 43.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1359
- ORF length:
- 1293
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag tgatcaaatt aaattcatta tggacagtct caataaggag ccctttagga 121 agaactataa tttaatcacg tttgattcct tggagccaat gcaactatta caagttctca 181 gtgatgttct ggctgagatt gacccaaagc aacttgtgga tatcagagag gagatgccag 241 agcagacagc caaacgaatg ttgagccttc ttggtattct taagtacaaa ccttcaggaa 301 atgccacaga tatgagtact tttcgtcagg gtttggtgat tggaagtaaa cctgtaattt 361 acccagtgct ccactggctt cttcagagga ctaatgaact gaagaaaaga gcatatttag 421 ctcgtttttt aataaaactt gaggtaccaa gtgagtttct tcaggatgaa actgtggctg 481 acaccaataa acagtatgaa gagttaatgg aagcctttaa aactttgcat aaagaatatg 541 agcagctcaa gatatctgga ttttctacag cagaaataag aaaggatatc agtgcaatgg 601 aagaagaaaa ggatcagctc attaagagag ttgaacattt gaagaaaagg gttgagacag 661 ctcagaatca tcaatggatg cttaaaatag caaggcaact tcgagttgaa aaagagagag 721 aagaatatct tgcacaacag aaacaggaac aaaagaatca gctatttcat gcagtgcaaa 781 gattgcaaag agtacaaaac cagctgaaaa gcatgcgcca agctgcagca gatgcaaagc 841 ctgaaagttt aatgaagagg ctagaggagg agataaaatt taatttatat atggtaactg 901 aaaaatttcc taaagaatta gaaaataaga aaaaggaatt acatttttta caaaaagtag 961 tttcagagcc agctatgggc cattctgaTC TTCTTGAACT TGAATCTAAA ATAAATGAAA 1021 TAAACACAGA AATTAACCAG TTGATTGAAA AGAAAATGAT GAGAAATGAG CCCATTGAAG 1081 GCAAACTCTC ACTGTATAGG CAACAGGCAT CTATCATTTC CCGTAAAAAA GAAGCCAAAG 1141 CTGAGGAACT TCAGGAGGCC AAGGAGAAGT TAGCCAGCCT AGAGAGAGAA GCATCAGTAA 1201 AGAGAAATCA GACCCGTGAA TTTGATGGTA CTGAAGTTTT AAAGGGAGAT GAGAGACAGG 1261 ATCTCACTCT GTCACCCAGG CTGGAGTGCG GTGGTGTGAT CATGGCTTAC TGCAGCCTGA 1321 AACTCCTAGG CTCAAGTGAT CCTCCTACCT CAGCCTCCTG CCCAACTTTC TTGTACAAAG 1381 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1441 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1501 ACGACTTCTC TTCATGCTTA CGGGAGCTAC GCGTTAAGTC gacaatcaac ctctggatta 1561 caaaatttgt gaaagatt