Construct: ORF TRCN0000480371
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012471.1_s317c1
- Derived from:
- ccsbBroadEn_01125
- DNA Barcode:
- CCGGTCCTATGTCCGGGTCCGCGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ORC4 (5000)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480371
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5000 | ORC4 | origin recognition complex ... | NM_001190879.2 | 100% | 100% | |
| 2 | human | 5000 | ORC4 | origin recognition complex ... | NM_001374270.1 | 100% | 100% | |
| 3 | human | 5000 | ORC4 | origin recognition complex ... | NM_002552.5 | 100% | 100% | |
| 4 | human | 5000 | ORC4 | origin recognition complex ... | NM_181741.4 | 100% | 100% | |
| 5 | human | 5000 | ORC4 | origin recognition complex ... | NM_181742.3 | 100% | 100% | |
| 6 | human | 5000 | ORC4 | origin recognition complex ... | XM_011511255.2 | 100% | 100% | |
| 7 | human | 5000 | ORC4 | origin recognition complex ... | NM_001190882.2 | 83% | 83% | 0_1ins222 |
| 8 | human | 5000 | ORC4 | origin recognition complex ... | NM_001190881.2 | 80.7% | 80.7% | 0_1ins252 |
| 9 | human | 5000 | ORC4 | origin recognition complex ... | NM_001374272.1 | 80.7% | 80.7% | 0_1ins252 |
| 10 | mouse | 26428 | Orc4 | origin recognition complex,... | NM_011958.3 | 88.1% | 88.9% | (many diffs) |
| 11 | mouse | 26428 | Orc4 | origin recognition complex,... | XM_006498063.3 | 88.1% | 88.9% | (many diffs) |
| 12 | mouse | 26428 | Orc4 | origin recognition complex,... | XM_006498064.3 | 88.1% | 88.9% | (many diffs) |
| 13 | mouse | 26428 | Orc4 | origin recognition complex,... | XM_006498065.3 | 88.1% | 88.9% | (many diffs) |
| 14 | mouse | 26428 | Orc4 | origin recognition complex,... | XM_006498066.3 | 75.9% | 76.8% | (many diffs) |
| 15 | mouse | 26428 | Orc4 | origin recognition complex,... | XR_001782774.1 | 59% | (many diffs) | |
| 16 | mouse | 26428 | Orc4 | origin recognition complex,... | XR_001782769.1 | 27.8% | (many diffs) | |
| 17 | mouse | 26428 | Orc4 | origin recognition complex,... | XR_001782772.1 | 27.8% | (many diffs) | |
| 18 | mouse | 26428 | Orc4 | origin recognition complex,... | XR_374094.2 | 27.2% | (many diffs) | |
| 19 | mouse | 26428 | Orc4 | origin recognition complex,... | NM_001177313.1 | 27.2% | 26.1% | (many diffs) |
| 20 | mouse | 26428 | Orc4 | origin recognition complex,... | XR_001782770.1 | 27% | (many diffs) | |
| 21 | mouse | 26428 | Orc4 | origin recognition complex,... | NR_033442.1 | 26.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1374
- ORF length:
- 1308
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag cagtcgtaaa tcaaagagta acagcttaat tcacacagag tgcctttcac 121 aggtacaaag aattttacgt gaaagatttt gtcgtcagag tccacatagt aacctatttg 181 gagtgcaagt acaatacaaa cacttaagtg agctgctgaa aagaactgct ctccatggag 241 agagtaactc tgtccttatt atcggacccc gaggatcagg aaaaactatg ttaataaatc 301 atgctttgaa agaactcatg gaaatagaag aagtgagtga aaatgtatta caagttcact 361 taaatggact gctgcagatc aatgacaaaa tcgccctaaa ggaaatcaca aggcagttaa 421 atctggaaaa tgtagttgga gataaagttt ttggaagctt tgctgaaaac ctttcatttc 481 ttctggaagc tttaaaaaaa ggtgaccgaa ctagcagttg cccagtgatc ttcatattag 541 atgaatttga tctttttgct catcataaaa accaaacact tctctataat ctttttgaca 601 tttctcagtc tgcacagacc ccaatagcag ttattggtct tacatgtaga ttggatattt 661 tggaactctt agaaaaaaga gtgaagtcaa gattttctca ccggcagata cacttaatga 721 attcatttgg ttttccacag tatgttaaaa tatttaaaga acagttatct ctacctgcag 781 agtttccaga caaggttttt gctgagaagt ggaatgaaaa tgttcagtat ctctcagaag 841 atagaagtgt gcaagaagta ctacagaagc atttcaatat cagcaaaaac ctgcggtcat 901 tacacatgct attgatgctt gctttaaatc gagtaacagc atcgcaccca tttatgactg 961 ccgtagatct aatggaagca agccaactgt gtagcatgga ctcgaaagca aatattgtac 1021 atggtctatc agtcttGGAA ATCTGTCTTA TAATAGCAAT GAAACATTTA AATGACATCT 1081 ATGAGGAAGA GCCATTTAAT TTTCAAATGG TCTATAATGA GTTTCAGAAG TTTGTTCAAA 1141 GGAAAGCACA TTCCGTTTAT AATTTTGAAA AACCTGTTGT CATGAAGGCT TTTGAACACT 1201 TGCAGCAATT AGAATTAATA AAGCCCATGG AAAGAACTTC AGGAAATTCA CAGAGAGAGT 1261 ACCAGCTGAT GAAACTGCTT TTGGATAATA CTCAAATTAT GAATGCTCTG CAGAAATATC 1321 CCAACTGTCC TACAGATGTG AGGCAGTGGG CAACATCCTC ACTAAGCTGG TTATGCCCAA 1381 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1441 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1501 TATCTTGTGG AAAGGACGAC CGGTCCTATG TCCGGGTCCG CGTACGCGTT AAGTCgacaa 1561 tcaacctctg gattacaaaa tttgtgaaag att