Transcript: Human NM_001374272.1

Homo sapiens origin recognition complex subunit 4 (ORC4), transcript variant 8, mRNA.

Source:
NCBI, updated 2019-09-30
Taxon:
Homo sapiens (human)
Gene:
ORC4 (5000)
Length:
6612
CDS:
440..1498

Additional Resources:

NCBI RefSeq record:
NM_001374272.1
NBCI Gene record:
ORC4 (5000)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001374272.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000155658 CCATTTATGACTGCCGTAGAT pLKO.1 1070 CDS 100% 4.950 6.930 N ORC4 n/a
2 TRCN0000238274 CTTACATGTAGATTGGATATT pLKO_005 761 CDS 100% 13.200 10.560 N Orc4 n/a
3 TRCN0000370574 CTATGAGGAAGAGCCATTTAA pLKO_005 1201 CDS 100% 15.000 10.500 N ORC4 n/a
4 TRCN0000365515 GAAAGCACATTCCGTTTATAA pLKO_005 1264 CDS 100% 15.000 10.500 N ORC4 n/a
5 TRCN0000365453 GAGTAACTCTGTCCTTATTAT pLKO_005 299 5UTR 100% 15.000 10.500 N ORC4 n/a
6 TRCN0000365514 CTCACCGGCAGATACACTTAA pLKO_005 819 CDS 100% 13.200 9.240 N ORC4 n/a
7 TRCN0000377590 ATGGACTGCTGCAGATCAATG pLKO_005 486 CDS 100% 10.800 7.560 N ORC4 n/a
8 TRCN0000370642 CCATTAACATGATATGCTAAA pLKO_005 1564 3UTR 100% 10.800 7.560 N ORC4 n/a
9 TRCN0000154989 GCGGTCATTACACATGCTATT pLKO.1 1015 CDS 100% 10.800 7.560 N ORC4 n/a
10 TRCN0000155091 GAGAGAGTAACTCTGTCCTTA pLKO.1 295 5UTR 100% 4.950 3.465 N ORC4 n/a
11 TRCN0000156991 GAGTGCCTTTCACAGGTACAA pLKO.1 165 5UTR 100% 4.950 3.465 N ORC4 n/a
12 TRCN0000150858 GTTCAGTATCTCTCAGAAGAT pLKO.1 944 CDS 100% 4.950 3.465 N ORC4 n/a
13 TRCN0000151984 CCAGTTAGAATGGCAATCATT pLKO.1 5452 3UTR 100% 5.625 2.813 Y LOC340211 n/a
14 TRCN0000136627 GCTCTCTGTTTGTCTGTTGTT pLKO.1 4831 3UTR 100% 4.950 2.475 Y FSIP2 n/a
15 TRCN0000156019 CATGGAATACTATGCAGCCAT pLKO.1 5790 3UTR 100% 2.640 1.320 Y LOC340211 n/a
16 TRCN0000155565 CCATTGTGTTACAGTTGCCTA pLKO.1 2285 3UTR 100% 2.640 1.320 Y ORC4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001374272.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01125 pDONR223 100% 80.7% 80.7% None 0_1ins252 n/a
2 ccsbBroad304_01125 pLX_304 0% 80.7% 80.7% V5 0_1ins252 n/a
3 TRCN0000480371 CCGGTCCTATGTCCGGGTCCGCGT pLX_317 26.2% 80.7% 80.7% V5 0_1ins252 n/a
4 ccsbBroadEn_11010 pDONR223 100% 37.6% 37.6% None 0_1ins252;493_1056del n/a
5 ccsbBroad304_11010 pLX_304 0% 37.6% 37.6% V5 0_1ins252;493_1056del n/a
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