Transcript: Human NM_002552.5

Homo sapiens origin recognition complex subunit 4 (ORC4), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-18
Taxon:
Homo sapiens (human)
Gene:
ORC4 (5000)
Length:
6572
CDS:
148..1458

Additional Resources:

NCBI RefSeq record:
NM_002552.5
NBCI Gene record:
ORC4 (5000)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_002552.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000155658 CCATTTATGACTGCCGTAGAT pLKO.1 1030 CDS 100% 4.950 6.930 N ORC4 n/a
2 TRCN0000238274 CTTACATGTAGATTGGATATT pLKO_005 721 CDS 100% 13.200 10.560 N Orc4 n/a
3 TRCN0000370574 CTATGAGGAAGAGCCATTTAA pLKO_005 1161 CDS 100% 15.000 10.500 N ORC4 n/a
4 TRCN0000365515 GAAAGCACATTCCGTTTATAA pLKO_005 1224 CDS 100% 15.000 10.500 N ORC4 n/a
5 TRCN0000365453 GAGTAACTCTGTCCTTATTAT pLKO_005 324 CDS 100% 15.000 10.500 N ORC4 n/a
6 TRCN0000365514 CTCACCGGCAGATACACTTAA pLKO_005 779 CDS 100% 13.200 9.240 N ORC4 n/a
7 TRCN0000377590 ATGGACTGCTGCAGATCAATG pLKO_005 446 CDS 100% 10.800 7.560 N ORC4 n/a
8 TRCN0000370642 CCATTAACATGATATGCTAAA pLKO_005 1524 3UTR 100% 10.800 7.560 N ORC4 n/a
9 TRCN0000154989 GCGGTCATTACACATGCTATT pLKO.1 975 CDS 100% 10.800 7.560 N ORC4 n/a
10 TRCN0000155091 GAGAGAGTAACTCTGTCCTTA pLKO.1 320 CDS 100% 4.950 3.465 N ORC4 n/a
11 TRCN0000156991 GAGTGCCTTTCACAGGTACAA pLKO.1 190 CDS 100% 4.950 3.465 N ORC4 n/a
12 TRCN0000150858 GTTCAGTATCTCTCAGAAGAT pLKO.1 904 CDS 100% 4.950 3.465 N ORC4 n/a
13 TRCN0000151984 CCAGTTAGAATGGCAATCATT pLKO.1 5412 3UTR 100% 5.625 2.813 Y LOC340211 n/a
14 TRCN0000136627 GCTCTCTGTTTGTCTGTTGTT pLKO.1 4791 3UTR 100% 4.950 2.475 Y FSIP2 n/a
15 TRCN0000156019 CATGGAATACTATGCAGCCAT pLKO.1 5750 3UTR 100% 2.640 1.320 Y LOC340211 n/a
16 TRCN0000155565 CCATTGTGTTACAGTTGCCTA pLKO.1 2245 3UTR 100% 2.640 1.320 Y ORC4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_002552.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01125 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_01125 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000480371 CCGGTCCTATGTCCGGGTCCGCGT pLX_317 26.2% 100% 100% V5 n/a
4 ccsbBroadEn_11010 pDONR223 100% 56.8% 56.6% None 233A>G;745_1308del n/a
5 ccsbBroad304_11010 pLX_304 0% 56.8% 56.6% V5 233A>G;745_1308del n/a
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