Construct: ORF TRCN0000480411
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008931.1_s317c1
- Derived from:
- ccsbBroadEn_07379
- DNA Barcode:
- CGGCCAGTATTTACATAGGTATCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DHRS3 (9249)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480411
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9249 | DHRS3 | dehydrogenase/reductase 3 | NM_004753.7 | 100% | 100% | |
| 2 | human | 9249 | DHRS3 | dehydrogenase/reductase 3 | NM_001319225.2 | 71.8% | 71.8% | 0_1ins255 |
| 3 | human | 9249 | DHRS3 | dehydrogenase/reductase 3 | NM_001324370.2 | 71.8% | 71.8% | 0_1ins255 |
| 4 | human | 9249 | DHRS3 | dehydrogenase/reductase 3 | XM_017002809.1 | 71.8% | 71.8% | 0_1ins255 |
| 5 | human | 9249 | DHRS3 | dehydrogenase/reductase 3 | XM_006711036.2 | 59.6% | 37.2% | 337_338ins359;548_558del |
| 6 | mouse | 20148 | Dhrs3 | dehydrogenase/reductase (SD... | NM_011303.6 | 88.1% | 95% | (many diffs) |
| 7 | mouse | 20148 | Dhrs3 | dehydrogenase/reductase (SD... | NM_001172424.1 | 80.7% | 87.4% | (many diffs) |
| 8 | mouse | 20148 | Dhrs3 | dehydrogenase/reductase (SD... | XM_006538678.2 | 80.7% | 87.4% | (many diffs) |
| 9 | mouse | 20148 | Dhrs3 | dehydrogenase/reductase (SD... | XM_006538679.3 | 72.2% | 75.5% | (many diffs) |
| 10 | mouse | 20148 | Dhrs3 | dehydrogenase/reductase (SD... | XM_017320087.1 | 63.5% | 69.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 972
- ORF length:
- 906
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gtggaaacgg ctgggcgcgc tggtgatgtt ccctctacag atgatctatc 121 tggtggtgaa agcagccgtc ggactggtgc tgcccgccaa gctgcgggac ctgtcgcggg 181 agaacgtcct catcaccggc ggcgggagag gcatcgggcg tcagctcgcc cgcgagttcg 241 cggagcgcgg cgccagaaag attgttctct ggggccggac tgagaaatgc ctgaaggaga 301 cgacggagga gatccggcag atgggcactg agtgccatta cttcatctgt gatgtgggca 361 accgggagga ggtgtaccag acggccaagg ccgtccggga gaaggtgggt gacatcacca 421 tcctggtgaa caatgccgcc gtggtccatg ggaagagcct aatggacagt gatgatgatg 481 ccctcctcaa gtcccaacac atcaacaccc tgggccagtt ctggaccacc aaggccttcc 541 tgccgcgtat gctggagctg cagaatggcc acatcgtgtg cctcaactcc gtgctggcac 601 tgtctgccaT CCCCGGTGCC ATCGACTACT GCACATCCAA AGCGTCAGCC TTCGCCTTCA 661 TGGAGAGCCT GACCCTGGGG CTGCTGGACT GTCCGGGAGT CAGCGCCACC ACAGTGCTGC 721 CCTTCCACAC CAGCACCGAG ATGTTCCAGG GCATGAGAGT CAGGTTTCCC AACCTCTTTC 781 CCCCACTGAA GCCGGAGACG GTGGCCCGGA GGACAGTGGA AGCTGTGCAG CTCAACCAGG 841 CCCTCCTCCT CCTCCCATGG ACAATGCATG CCCTCGTTAT CTTGAAAAGC ATACTTCCAC 901 AGGCTGCACT CGAGGAGATC CACAAATTCT CAGGAACCTA CACCTGCATG AACACTTTCA 961 AAGGGCGGAC ATACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1021 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1081 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACGG CCAGTATTTA CATAGGTATC 1141 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t