Transcript: Human XM_017002809.1

PREDICTED: Homo sapiens dehydrogenase/reductase 3 (DHRS3), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
DHRS3 (9249)
Length:
1247
CDS:
164..817

Additional Resources:

NCBI RefSeq record:
XM_017002809.1
NBCI Gene record:
DHRS3 (9249)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017002809.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000036529 GCACAGGACTGATGGGTATAA pLKO.1 981 3UTR 100% 13.200 9.240 N DHRS3 n/a
2 TRCN0000290812 GCACAGGACTGATGGGTATAA pLKO_005 981 3UTR 100% 13.200 9.240 N DHRS3 n/a
3 TRCN0000036532 ACCTGCATGAACACTTTCAAA pLKO.1 785 CDS 100% 5.625 3.938 N DHRS3 n/a
4 TRCN0000290810 ACCTGCATGAACACTTTCAAA pLKO_005 785 CDS 100% 5.625 3.938 N DHRS3 n/a
5 TRCN0000036531 CCGGACTGAGAAATGCCTGAA pLKO.1 118 5UTR 100% 4.050 2.835 N DHRS3 n/a
6 TRCN0000290811 CCGGACTGAGAAATGCCTGAA pLKO_005 118 5UTR 100% 4.050 2.835 N DHRS3 n/a
7 TRCN0000036530 CCACAAATTCTCAGGAACCTA pLKO.1 763 CDS 100% 3.000 2.100 N DHRS3 n/a
8 TRCN0000290886 CCACAAATTCTCAGGAACCTA pLKO_005 763 CDS 100% 3.000 2.100 N DHRS3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017002809.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07379 pDONR223 100% 71.8% 71.8% None 0_1ins255 n/a
2 ccsbBroad304_07379 pLX_304 0% 71.8% 71.8% V5 0_1ins255 n/a
3 TRCN0000480411 CGGCCAGTATTTACATAGGTATCG pLX_317 40.2% 71.8% 71.8% V5 0_1ins255 n/a
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