Construct: ORF TRCN0000480412
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008821.1_s317c1
- Derived from:
- ccsbBroadEn_11305
- DNA Barcode:
- ACAAGATCCAATTGACGCAGGTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SUCLG2 (8801)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480412
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8801 | SUCLG2 | succinate-CoA ligase GDP-fo... | NM_003848.3 | 88.8% | 88.8% | 1_144del |
| 2 | human | 8801 | SUCLG2 | succinate-CoA ligase GDP-fo... | NM_001177599.1 | 82% | 80.3% | (many diffs) |
| 3 | human | 8801 | SUCLG2 | succinate-CoA ligase GDP-fo... | XM_017007420.2 | 81.6% | 80.6% | (many diffs) |
| 4 | human | 8801 | SUCLG2 | succinate-CoA ligase GDP-fo... | XR_001740348.2 | 45.7% | 1_169del;1088_1257del;1492_2519del | |
| 5 | human | 8801 | SUCLG2 | succinate-CoA ligase GDP-fo... | XR_001740350.2 | 21.7% | (many diffs) | |
| 6 | mouse | 20917 | Suclg2 | succinate-Coenzyme A ligase... | NM_001326558.1 | 88.5% | 94.5% | (many diffs) |
| 7 | mouse | 20917 | Suclg2 | succinate-Coenzyme A ligase... | NM_011507.3 | 78.5% | 83.8% | (many diffs) |
| 8 | mouse | 20917 | Suclg2 | succinate-Coenzyme A ligase... | XM_017321481.1 | 54.3% | 56.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1218
- ORF length:
- 1152
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tgacaacgga gtgagagttc aaagattctt tgtagcagac actgcaaatg 121 aagctctcga ggctgctaag agactaaatg caaaagaaat tgttttaaaa gcccagatct 181 tagctggagg aagaggaaaa ggtgtcttca atagtggttt gaaaggaggt gttcatttaa 241 caaaagaccc taatgttgtg ggacagctgg ctaaacagat gattgggtac aatctagcga 301 caaaacaaac tccaaaagaa ggtgtgaaag ttaacaaggt gatggttgct gaagccttgg 361 atatttccag agaaacctac ctggcaattc tgatggaccg gtcctgcaat ggccccgtgc 421 tggtgggcag cccccagggg ggcgtcgaca ttgaagaggt ggctgcttca aacccggagc 481 tcatttttaa ggagcaaatt gacatttttg aaggaataaa ggacagccaa gctcagcgga 541 tggccgaaaa tctaggcttc gttgggcctt tgaaaagcca ggctgcagat caaattacga 601 agctgtataa tctcttcctg aaaattgatg ctactcaggt ggaagtgaat ccctttggtg 661 aaactccaga aggacaagtt gtctgttttg atgccaagat aaactttgat gacaacgcag 721 aattccgaca aaaagacata tttgctatgg acgacaaatc agagaatgag cccattgaaa 781 atgaagctgc caaatatgat ctaaaataca taggactaga tgggaacatt gcctgctttg 841 tgaatggtgc tgggctcgcc atggctactt gtgatatcat tttccttaat ggtgggaagc 901 cagccaactt cttggatctt GGAGGTGGTG TAAAGGAAGC TCAAGTATAT CAAGCATTCA 961 AATTGCTCAC AGCTGATCCT AAGGTTGAAG CCATCCTTGT CAATATATTT GGTGGTATCG 1021 TCAACTGTGC CATCATTGCC AATGGGATCA CCAAAGCCTG CCGGGAGCTA GAACTCAAGG 1081 TGCCCCTGGT GGTCCGGCTT GAAGGAACCA ACGTCCAAGA GGCCCAGAAG ATACTCAACA 1141 ACAGCGGACT CCCCATTACT TCAGCCATTG ACCTGGAGGA TGCAGCCAAG AAGGCTGTGG 1201 CCAGTGTGGC CAAGAAGTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1261 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1321 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAACAAGAT CCAATTGACG 1381 CAGGTATACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt