Construct: ORF TRCN0000480419
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005131.1_s317c1
- Derived from:
- ccsbBroadEn_07648
- DNA Barcode:
- AGTGTAATCATCCGAGAGCCTTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDLIM5 (10611)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480419
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 10611 | PDLIM5 | PDZ and LIM domain 5 | NM_006457.5 | 99.8% | 99.4% | 1033G>A;1141A>G;1475G>A |
2 | human | 10611 | PDLIM5 | PDZ and LIM domain 5 | NM_001011513.4 | 81.5% | 81.2% | (many diffs) |
3 | human | 10611 | PDLIM5 | PDZ and LIM domain 5 | NM_001256428.2 | 79.3% | 79% | (many diffs) |
4 | human | 10611 | PDLIM5 | PDZ and LIM domain 5 | NM_001256427.2 | 79% | 78.7% | (many diffs) |
5 | human | 10611 | PDLIM5 | PDZ and LIM domain 5 | NM_001256426.1 | 66.2% | 65.9% | (many diffs) |
6 | human | 10611 | PDLIM5 | PDZ and LIM domain 5 | NM_001256425.1 | 45.3% | 45.1% | 0_1ins975;166A>G;500G>A |
7 | human | 10611 | PDLIM5 | PDZ and LIM domain 5 | NM_001011515.2 | 36.1% | 33% | (many diffs) |
8 | human | 10611 | PDLIM5 | PDZ and LIM domain 5 | NM_001011516.2 | 33.8% | 29.5% | (many diffs) |
9 | mouse | 56376 | Pdlim5 | PDZ and LIM domain 5 | NM_019808.3 | 85.6% | 88.4% | (many diffs) |
10 | mouse | 56376 | Pdlim5 | PDZ and LIM domain 5 | XM_006501746.1 | 84% | 86.8% | (many diffs) |
11 | mouse | 56376 | Pdlim5 | PDZ and LIM domain 5 | XM_006501743.1 | 78% | 80.4% | (many diffs) |
12 | mouse | 56376 | Pdlim5 | PDZ and LIM domain 5 | XM_006501741.1 | 73.9% | 75.3% | (many diffs) |
13 | mouse | 56376 | Pdlim5 | PDZ and LIM domain 5 | XM_006501742.1 | 73.5% | 75.6% | (many diffs) |
14 | mouse | 56376 | Pdlim5 | PDZ and LIM domain 5 | XM_006501740.1 | 71.2% | 72.2% | (many diffs) |
15 | mouse | 56376 | Pdlim5 | PDZ and LIM domain 5 | XM_006501739.1 | 70.6% | 71.6% | (many diffs) |
16 | mouse | 56376 | Pdlim5 | PDZ and LIM domain 5 | XM_006501738.1 | 70.1% | 71.1% | (many diffs) |
17 | mouse | 56376 | Pdlim5 | PDZ and LIM domain 5 | NM_001190854.1 | 69.4% | 72.9% | (many diffs) |
18 | mouse | 56376 | Pdlim5 | PDZ and LIM domain 5 | XM_006501748.1 | 68.7% | 72.2% | (many diffs) |
19 | mouse | 56376 | Pdlim5 | PDZ and LIM domain 5 | NM_001190855.1 | 64.7% | 67.8% | (many diffs) |
20 | mouse | 56376 | Pdlim5 | PDZ and LIM domain 5 | NM_001190856.1 | 61% | 63.2% | (many diffs) |
21 | mouse | 56376 | Pdlim5 | PDZ and LIM domain 5 | NM_001190852.1 | 57.9% | 59.6% | (many diffs) |
22 | mouse | 56376 | Pdlim5 | PDZ and LIM domain 5 | XM_006501745.1 | 57.4% | 59.1% | (many diffs) |
23 | mouse | 56376 | Pdlim5 | PDZ and LIM domain 5 | XM_006501744.1 | 57% | 58.7% | (many diffs) |
24 | mouse | 56376 | Pdlim5 | PDZ and LIM domain 5 | XM_017319659.1 | 55.8% | 56.8% | (many diffs) |
25 | mouse | 56376 | Pdlim5 | PDZ and LIM domain 5 | NM_019809.3 | 49.2% | 46.2% | (many diffs) |
26 | mouse | 56376 | Pdlim5 | PDZ and LIM domain 5 | NM_001190857.1 | 32.8% | 30.7% | (many diffs) |
27 | mouse | 56376 | Pdlim5 | PDZ and LIM domain 5 | XM_006501751.2 | 32.8% | 30.7% | (many diffs) |
28 | mouse | 56376 | Pdlim5 | PDZ and LIM domain 5 | NM_022554.3 | 32.5% | 30.5% | (many diffs) |
29 | mouse | 56376 | Pdlim5 | PDZ and LIM domain 5 | XM_006501750.2 | 32.5% | 30.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1854
- ORF length:
- 1788
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag caactacagt gtgtcactgg ttggcccagc tccttggggt ttccggctgc 121 agggcggtaa ggatttcaac atgcctctga caatctctag tctaaaagat ggcggcaagg 181 cagcccaggc aaatgtaaga ataggcgatg tggttctcag cattgatgga ataaatgcac 241 aaggaatgac tcatcttgaa gcccagaata agattaaggg ttgtacaggc tctttgaata 301 tgactctgca aagagcatct gctgcaccca agcctgagcc ggttcctgtt caaaagggag 361 aacctaaaga agtagttaaa cctgtgccca ttacatctcc tgctgtgtcc aaagtcactt 421 ccacaaacaa catggcctac aataaggcac cacggccttt tggttctgtg tcttcaccaa 481 aagtcacatc catcccatca ccatcgtctg ccttcacccc agcccatgcg accacctcat 541 cacatgcttc cccttcaccc gtggctgccg tcactcctcc cctgttcgct gcatctggac 601 tgcatgctaa tgccaatctt agtgctgacc agtctccatc tgcactgagc gctggtaaaa 661 ctgcagttaa tgtcccacgg cagcccacag tcaccagcgt gtgttccgag acttctcagg 721 agctagcaga gggacagaga agaggatccc agggtgacag taaacagcaa aatggcccac 781 caagaaaaca cattgtggag cgctatacag agttttatca tgtacccact cacagtgatg 841 ccagcaagaa gagactgatt gaggatactg aagactggcg tccaaggact ggaacaactc 901 agtctcgctc tttccgaatc cttgcccaga tcactgggac tgaacatttg aaagaatctg 961 aagccgataa tacaaagaag gcaaataact ctcaggagcc ttctccgcag ttggcttcct 1021 cggtagcttc cacacggagc atgcccgaga gcctggacag cccaacctct ggcagaccag 1081 gggttaccag cctcacaact gcagctgcct tcaagcctgt aggatccact ggcgtcatca 1141 agtcaccaag ctggcaacgg ccaaaccaag gagtaccttc cactggaaga atctcaaaca 1201 gcgctgctta ctcaggatca gtggcaccag ccaactcagc tttgggacaa acccagccaa 1261 gtgaccagga cactttagtg caaagagctg agcacattcc agcagggaaa cgaactccga 1321 tgtgcgccca ttgtaaccag gtcatcagag gaccattctt agtggcactg gggaaatctt 1381 ggcacccaga agaattcaac tgcgctcact gcaaaaatac aatggccTAC ATTGGATTTG 1441 TAGAGGAGAA AGGAGCCCTG TATTGTGAGC TGTGCTATGA GAAATTCTTT GCCCCTGAAT 1501 GTGGTCGATG CCAAAGGAAG ATCCTTGGAG AAGTCATCAA TGCGTTGAAA CAAACTTGGC 1561 ATGTTTCCTG TTTTGTGTGT GTAGCCTGTG GAAAGCCCAT TCGGAACAAT GTTTTTCACT 1621 TGGAGGATGG TGAACCCTAC TGTGAGACTG ATTATTATGC CCTCTTTGGT ACTATATGCC 1681 ATGGATGTGA ATTTCCCATA GAAGCTGGTG ACATGTTCCT GGAAGCTCTG GGCTACACCT 1741 GGCATGACAC TTGCTTTGTA TGCTCAGTGT GTTGTGAAAG TTTGGAAGGT CAGACCTTTT 1801 TCTCCAAGAA GGACAAGCCC CTGTGTAAGA AACATGCTCA TTCTGTGAAT TTTTGCCCAA 1861 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1921 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1981 TATCTTGTGG AAAGGACGAA GTGTAATCAT CCGAGAGCCT TTTACGCGTT AAGTCgacaa 2041 tcaacctctg gattacaaaa tttgtgaaag att