Construct: ORF TRCN0000480471
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008089.1_s317c1
- Derived from:
- ccsbBroadEn_06982
- DNA Barcode:
- CTCGCGGACACAGTTATGTTCTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SNRNP70 (6625)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480471
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6625 | SNRNP70 | small nuclear ribonucleopro... | NM_003089.6 | 99.9% | 100% | 933C>T |
| 2 | human | 6625 | SNRNP70 | small nuclear ribonucleopro... | NM_001301069.2 | 97.8% | 97.9% | 663_664ins27;906C>T |
| 3 | human | 6625 | SNRNP70 | small nuclear ribonucleopro... | XM_011527240.2 | 75.6% | 71.3% | (many diffs) |
| 4 | human | 6625 | SNRNP70 | small nuclear ribonucleopro... | XM_011527241.2 | 27.9% | 27.9% | 0_1ins945 |
| 5 | mouse | 20637 | Snrnp70 | small nuclear ribonucleopro... | NM_009224.5 | 84.2% | 94.8% | (many diffs) |
| 6 | mouse | 20637 | Snrnp70 | small nuclear ribonucleopro... | XM_006540732.3 | 84.2% | 94.8% | (many diffs) |
| 7 | mouse | 20637 | Snrnp70 | small nuclear ribonucleopro... | XM_006540733.3 | 43.2% | 45.2% | (many diffs) |
| 8 | mouse | 20637 | Snrnp70 | small nuclear ribonucleopro... | XM_011250831.2 | 43.2% | 45.2% | (many diffs) |
| 9 | mouse | 20637 | Snrnp70 | small nuclear ribonucleopro... | NR_126538.1 | 36.4% | (many diffs) | |
| 10 | mouse | 20637 | Snrnp70 | small nuclear ribonucleopro... | NR_126539.1 | 27.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1380
- ORF length:
- 1311
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gacccagttc ctgccgccca accttctggc cctctttgcc ccccgtgacc 121 ctattccata cctgccaccc ctggagaaac tgccacatga aaaacaccac aatcaacctt 181 attgtggcat tgcgccgtac attcgagagt ttgaggaccc tcgagatgcc cctcctccaa 241 ctcgtgctga aacccgagag gagcgcatgg agaggaaaag acgggaaaag attgagcggc 301 gacagcaaga agtggagaca gagcttaaaa tgtgggaccc tcacaatgat cccaatgctc 361 agggggatgc cttcaagact ctcttcgtgg cgagagtgaa ttatgacaca acagaatcca 421 agctccggag agagtttgag gtgtacggac ctatcaaaag aatacacatg gtctacagta 481 agcggtcagg aaagccccgt ggctatgcct tcatcgagta cgaacacgag cgagacatgc 541 actccgctta caaacacgca gatggcaaga agattgatgg caggagggtc cttgtggacg 601 tggagagggg ccgaaccgtg aagggctgga ggccccggcg gctaggagga ggcctcggtg 661 gtaccagaag aggaggggct gatgtgaaca tccggcattc aggccgcgat gacacctccc 721 gctacgatga gaggcccggc ccctccccgc ttccgcacag ggaccgggac cgggaccgtg 781 agcgggagcg cagagagcgg agccgggagc gagacaagga gcgagaacgg cgacgctccc 841 gctcccggga ccggcggagg cgctcacgga gtcgcgacaa ggaggagcgg aggcgctcca 901 gggagcggag caaggacaag gaccgggacc ggaagcggcg aagcagccgg agtcgggagc 961 gggcccggcg ggagcgggag cgcaaggagg agctgcgtgg tggcggtggc gacatggcgg 1021 agcccTCCGA GGCGGGTGAC GCGCCCCCTG ATGATGGGCC TCCAGGGGAG CTCGGGCCTG 1081 ACGGCCCTGA CGGTCCAGAG GAAAAGGGCC GGGATCGTGA CCGGGAGCGA CGGCGGAGCC 1141 ACCGGAGCGA GCGCGAGCGG CGCCGGGACC GGGATCGTGA CCGTGACCGT GACCGCGAGC 1201 ACAAACGGGG GGAGCGGGGC AGTGAGCGGG GCAGGGATGA GGCCCGAGGT GGGGGCGGTG 1261 GCCAGGACAA CGGGCTGGAG GGTCTGGGCA ACGACAGCCG AGACATGTAC ATGGAGTCTG 1321 AGGGCGGCGA CGGCTACCTG GCTCCGGAGA ATGGGTATTT GATGGAGGCT GCGCCGGAGT 1381 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1441 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1501 TTTATATATC TTGTGGAAAG GACGACTCGC GGACACAGTT ATGTTCTCAA CGCGTTAAGT 1561 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt