Construct: ORF TRCN0000480516

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019051.3_s317c1
Derived from:
ccsbBroadEn_14649
DNA Barcode:
TTTGAGTTCGTACCGCCTCCTTGT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
FYN (2534)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000480516
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2534 FYN FYN proto-oncogene, Src fam... NM_153048.3 99.8% 100% 1445_1446delTG
2 human 2534 FYN FYN proto-oncogene, Src fam... XM_005266892.4 99.8% 100% 1445_1446delTG
3 human 2534 FYN FYN proto-oncogene, Src fam... NM_153047.4 90.1% 90.2% 697_852del;1601_1602delTG
4 human 2534 FYN FYN proto-oncogene, Src fam... NM_001370529.1 89.6% 89.7% 697_861del;1610_1611delTG
5 human 2534 FYN FYN proto-oncogene, Src fam... NM_002037.5 89.6% 89.7% 697_861del;1610_1611delTG
6 human 2534 FYN FYN proto-oncogene, Src fam... XM_017010650.1 89.6% 89.7% 697_861del;1610_1611delTG
7 human 2534 FYN FYN proto-oncogene, Src fam... XM_017010651.1 89.6% 89.7% 697_861del;1610_1611delTG
8 human 2534 FYN FYN proto-oncogene, Src fam... XM_017010652.1 89.6% 89.7% 697_861del;1610_1611delTG
9 human 2534 FYN FYN proto-oncogene, Src fam... XM_017010653.1 89.6% 89.7% 697_861del;1610_1611delTG
10 human 2534 FYN FYN proto-oncogene, Src fam... XM_017010655.2 58.9% 48.9% (many diffs)
11 mouse 14360 Fyn Fyn proto-oncogene NM_001122892.1 83.3% 89.7% (many diffs)
12 mouse 14360 Fyn Fyn proto-oncogene NM_008054.2 83.3% 89.7% (many diffs)
13 mouse 14360 Fyn Fyn proto-oncogene NM_001122893.1 82.9% 89.1% (many diffs)
14 mouse 14360 Fyn Fyn proto-oncogene XM_006512539.3 82.9% 89.1% (many diffs)
15 mouse 14360 Fyn Fyn proto-oncogene XM_006512540.3 82.9% 89.1% (many diffs)
16 mouse 14360 Fyn Fyn proto-oncogene XM_011243117.2 82.9% 89.1% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1515
ORF length:
1446
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gggctgtgtg caatgtaagg ataaagaagc aacaaaactg acggaggaga
121gggacggcag cctgaaccag agctctgggt accgctatgg cacagacccc acccctcagc
181actaccccag cttcggtgtg acctccatcc ccaactacaa caacttccac gcagccgggg
241gccaaggact caccgtcttt ggaggtgtga actcttcgtc tcatacgggg accttgcgta
301cgagaggagg aacaggagtg acactctttg tggcccttta tgactatgaa gcacggacag
361aagatgacct gagttttcac aaaggagaaa aatttcaaat attgaacagc tcggaaggag
421attggtggga agcccgctcc ttgacaactg gagagacagg ttacattccc agcaattatg
481tggctccagt tgactctatc caggcagaag agtggtactt tggaaaactt ggccgaaaag
541atgctgagcg acagctattg tcctttggaa acccaagagg tacctttctt atccgcgaga
601gtgaaaccac caaaggtgcc tattcacttt ctatccgtga ttgggatgat atgaaaggag
661accatgtcaa acattataaa attcgcaaac ttgacaatgg tggatactac attaccaccc
721gggcccagtt tgaaacactt cagcagcttg tacaacatta ctcaggtacc tggaatggaa
781acacaaaagt agccataaag actcttaaac caggcacaat gtcccccgaa tcattccttg
841aggaagcgca gatcatgaag aagctgaagc acgacaagct ggtccagctc tatgcagtgg
901tgtctgagga gcccatctac atcgtcaccg agtatatgaa caaaggaagt ttactggatt
961tcttaaaaga tggagaagga agagctctga aattaccaaa tcttgtggac atggcagcac
1021aggtggctgc aggaatggct tacatcgagc gcatgaatta tatccataga gatctgcgat
1081cagcaaacat tctagtgggG AATGGACTCA TATGCAAGAT TGCTGACTTC GGATTGGCCC
1141GATTGATAGA AGACAATGAG TACACAGCAA GACAAGGTGC AAAGTTCCCC ATCAAGTGGA
1201CGGCCCCCGA GGCAGCCCTG TACGGGAGGT TCACAATCAA GTCTGACGTG TGGTCTTTTG
1261GAATCTTACT CACAGAGCTG GTCACCAAAG GAAGAGTGCC ATACCCAGGC ATGAACAACC
1321GGGAGGTGCT GGAGCAGGTG GAGCGAGGCT ACAGGATGCC CTGCCCGCAG GACTGCCCCA
1381TCTCTCTGCA TGAGCTCATG ATCCACTGCT GGAAAAAGGA CCCTGAAGAA CGCCCCACTT
1441TTGAGTACTT GCAGAGCTTC CTGGAAGACT ACTTTACCGC GACAGAGCCC CAGTACCAAC
1501CTGGTGAAAA CCTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA
1561ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT
1621CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATTT GAGTTCGTAC CGCCTCCTTG
1681TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)