Construct: ORF TRCN0000480520
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016088.1_s317c1
- Derived from:
- ccsbBroadEn_14947
- DNA Barcode:
- ACATAAGTTTACCCGAGAACGCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TLK1 (9874)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480520
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9874 | TLK1 | tousled like kinase 1 | NM_012290.5 | 99.9% | 100% | 900C>T |
| 2 | human | 9874 | TLK1 | tousled like kinase 1 | NM_001136554.2 | 93.6% | 93.7% | 0_1ins144;756C>T |
| 3 | human | 9874 | TLK1 | tousled like kinase 1 | XM_011512238.3 | 91.7% | 91.7% | 900C>T;980_981ins189 |
| 4 | human | 9874 | TLK1 | tousled like kinase 1 | NM_001136555.2 | 86.5% | 85.6% | (many diffs) |
| 5 | human | 9874 | TLK1 | tousled like kinase 1 | XM_017005422.2 | 71.6% | 71.6% | 0_1ins651;249C>T |
| 6 | human | 9874 | TLK1 | tousled like kinase 1 | XM_024453261.1 | 71.6% | 71.6% | 0_1ins651;249C>T |
| 7 | human | 9874 | TLK1 | tousled like kinase 1 | XM_024453262.1 | 71.6% | 71.6% | 0_1ins651;249C>T |
| 8 | human | 9874 | TLK1 | tousled like kinase 1 | XR_001739084.2 | 46.9% | (many diffs) | |
| 9 | human | 9874 | TLK1 | tousled like kinase 1 | XR_001739085.2 | 46.1% | (many diffs) | |
| 10 | mouse | 228012 | Tlk1 | tousled-like kinase 1 | NM_172664.3 | 91.9% | 98.1% | (many diffs) |
| 11 | mouse | 228012 | Tlk1 | tousled-like kinase 1 | XM_006499189.3 | 87.5% | 93.4% | (many diffs) |
| 12 | mouse | 228012 | Tlk1 | tousled-like kinase 1 | XM_006499188.3 | 87.4% | 92.6% | (many diffs) |
| 13 | mouse | 228012 | Tlk1 | tousled-like kinase 1 | XM_006499190.3 | 85.7% | 92% | (many diffs) |
| 14 | mouse | 228012 | Tlk1 | tousled-like kinase 1 | XM_006499191.1 | 65.4% | 70.4% | (many diffs) |
| 15 | mouse | 228012 | Tlk1 | tousled-like kinase 1 | XM_006499193.1 | 65.4% | 70.4% | (many diffs) |
| 16 | mouse | 228012 | Tlk1 | tousled-like kinase 1 | XM_017317580.1 | 65.4% | 70.4% | (many diffs) |
| 17 | mouse | 228012 | Tlk1 | tousled-like kinase 1 | XM_017317581.1 | 65.4% | 70.4% | (many diffs) |
| 18 | mouse | 228012 | Tlk1 | tousled-like kinase 1 | XR_374441.3 | 45.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2364
- ORF length:
- 2298
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag tgtccaaagt agcagtggaa gtttggaggg gccgccatct tggtcccagc 121 tctccacgtc tccaaccccg ggctcggcgg cggcggccag gtccctgctg aatcacacgc 181 cgccatccgg gaggcccagg gaaggtgcaa tggatgagct tcatagtctg gatccaagaa 241 ggcaagagtt attggaagct agatttactg gagttgcaag tgggagcact ggaagtacgg 301 gcagttgcag tgttggagct aaagcctcaa caaataacga aagctctaat cacagttttg 361 gaagcttggg atctttaagt gacaaagaat cagagacacc ggagaagaaa caatcggaat 421 catccagggg aagaaagaga aaagcagaaa accagaatga aagtagtcag ggaaaaagta 481 ttgggggacg tggccacaaa attagcgact attttgaata ccagggtgga aatggctcaa 541 gtccagtaag aggcatacct cctgcaatcc gttctcctca aaattcacat tcacattcca 601 ctccttcctc atctgttcga ccgaatagcc cttctcctac tgcattagca tttggggacc 661 accctattgt acaaccaaag caattatcct ttaaaattat tcagactgat ctcacaatgc 721 tgaaattagc agcattagaa agtaataaaa tccaggacct ggaaaagaag gagggacgta 781 tagatgattt gctcagggct aactgtgatc tcagacggca aatagatgaa caacaaaaat 841 tacttgaaaa atacaaagaa cgattaaata agtgcatatc aatgagcaag aaacttctta 901 ttgaaaagag tacacaagaa aagctgtcaa gcagagagaa gagtatgcaa gatcgattac 961 gccttgggca ctttacaaca gttagacatg gcgcttcatt tactgaacaa tggacagatg 1021 gttttgcatt tcagaatctt gtgaagcaac aagaatgggt gaatcagcaa agggaagata 1081 ttgaaaggca aaggaaactt ctagccaaac gcaaacctcc cacagctaat aattctcagg 1141 caccctctac caattctgaa ccaaaacaaa ggaaaaacaa agcagtcaat ggagcagaga 1201 atgatccctt tgttagacca aatttaccac aactgttgac tttggcagaa tatcatgaac 1261 aggaagaaat tttcaaactt agactaggac atctcaaaaa ggaagaggca gaaatccagg 1321 cagaacttga acgtttggaa agagtcagaa atcttcacat acgtgagctg aaaagaataa 1381 acaatgaaga taattcacag ttcaaagatc acccaacatt aaatgaaaga tatttattac 1441 ttcatctgct tggtagaggt ggctttagtg aagtgtataa ggcttttgac ctttatgaac 1501 aaagatatgc tgctgtgaag atacatcagc ttaataaaag ctggagagat gagaagaaag 1561 aaaactacca caaacatgcc tgcagagagt atagaataca caaagaactg gatcacccca 1621 gaatagttaa actctatgat tatttctcct tggatacaga tacgttttgt acagtgttag 1681 aatactgtga aggcaatgac ttggatttct atctgaagca acacaagtta atgtcagaga 1741 aagaagctcg gtctattgta atgcagattg taaatgcact aagatatctc aatgagatca 1801 aaccccctat tatacattat gatcttaagc caggaaacat cctactggta gatggaacag 1861 catgtggtga aatcaaaatc actgattttg gtctgtccaa gattatggat gatgatagct 1921 atggtgtaga tggaatggat ctaacttccc agggggcagg cacttactgg tatttacctc 1981 ctgagtgttt tgtagttgga aaagagccac caaagatttc caacaaggtt gatgtatggt 2041 cggttGGAGT CATCTTCTTT CAGTGTCTTT ATGGTAGAAA GCCATTTGGT CACAATCAAT 2101 CTCAACAAGA CATTCTTCAA GAAAATACAA TATTAAAAGC CACAGAAGTC CAGTTCCCTG 2161 TAAAACCGGT TGTAAGCAGT GAAGCCAAGG CATTTATAAG ACGCTGTTTG GCATATCGAA 2221 AAGAAGATCG ATTTGATGTG CACCAGCTGG CAAATGACCC ATACCTTCTC CCACACATGA 2281 GAAGATCAAA TTCTTCAGGA AACCTACACA TGGCTGGGCT GACAGCATCC CCTACACCCC 2341 CTTCTTCAAG CATAATTACT TACTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 2401 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 2461 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA CATAAGTTTA 2521 CCCGAGAACG CGGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 2581 att