Construct: ORF TRCN0000480527
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014311.2_s317c1
- Derived from:
- ccsbBroadEn_02730
- DNA Barcode:
- TTCGGAAGTCTATGAAGTACAAAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- DIP2A (23181)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480527
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23181 | DIP2A | disco interacting protein 2... | NM_001146115.2 | 94.3% | 90.7% | (many diffs) |
| 2 | human | 23181 | DIP2A | disco interacting protein 2... | NM_206890.3 | 89.6% | 86% | (many diffs) |
| 3 | human | 23181 | DIP2A | disco interacting protein 2... | NM_206891.3 | 89.1% | 89.1% | (many diffs) |
| 4 | human | 23181 | DIP2A | disco interacting protein 2... | NM_001353944.2 | 87.3% | 82.1% | (many diffs) |
| 5 | human | 23181 | DIP2A | disco interacting protein 2... | XM_011529502.1 | 85.5% | 82.1% | (many diffs) |
| 6 | human | 23181 | DIP2A | disco interacting protein 2... | XM_017028305.1 | 85.5% | 82.1% | (many diffs) |
| 7 | human | 23181 | DIP2A | disco interacting protein 2... | NM_206889.3 | 84.8% | 81.4% | (many diffs) |
| 8 | human | 23181 | DIP2A | disco interacting protein 2... | XM_011529501.1 | 62.5% | 59.8% | (many diffs) |
| 9 | human | 23181 | DIP2A | disco interacting protein 2... | XM_017028304.1 | 54.8% | 52.4% | (many diffs) |
| 10 | human | 23181 | DIP2A | disco interacting protein 2... | XM_017028303.1 | 51.3% | 48.9% | (many diffs) |
| 11 | human | 23181 | DIP2A | disco interacting protein 2... | XM_017028301.1 | 50.8% | 48.5% | (many diffs) |
| 12 | human | 23181 | DIP2A | disco interacting protein 2... | XM_017028300.1 | 50% | 47.7% | (many diffs) |
| 13 | human | 23181 | DIP2A | disco interacting protein 2... | XM_017028298.2 | 49.6% | 47.3% | (many diffs) |
| 14 | human | 23181 | DIP2A | disco interacting protein 2... | XM_017028297.1 | 48.5% | 46.3% | (many diffs) |
| 15 | human | 23181 | DIP2A | disco interacting protein 2... | NM_001353943.1 | 48.2% | 46% | (many diffs) |
| 16 | human | 23181 | DIP2A | disco interacting protein 2... | NM_015151.4 | 48.2% | 46% | (many diffs) |
| 17 | human | 23181 | DIP2A | disco interacting protein 2... | XM_017028295.2 | 48.2% | 46% | (many diffs) |
| 18 | human | 23181 | DIP2A | disco interacting protein 2... | NM_001353942.1 | 48.2% | 46% | (many diffs) |
| 19 | human | 23181 | DIP2A | disco interacting protein 2... | XM_017028294.2 | 48.2% | 46% | (many diffs) |
| 20 | human | 23181 | DIP2A | disco interacting protein 2... | NM_001146116.1 | 48% | 45.8% | (many diffs) |
| 21 | human | 23181 | DIP2A | disco interacting protein 2... | XM_017028293.1 | 47.2% | 45% | (many diffs) |
| 22 | human | 23181 | DIP2A | disco interacting protein 2... | XM_011529492.2 | 47.2% | 45% | (many diffs) |
| 23 | human | 23181 | DIP2A | disco interacting protein 2... | XM_017028291.1 | 47.2% | 45% | (many diffs) |
| 24 | human | 23181 | DIP2A | disco interacting protein 2... | XM_017028292.1 | 47.2% | 45% | (many diffs) |
| 25 | human | 23181 | DIP2A | disco interacting protein 2... | XM_017028302.2 | 46.1% | 42.9% | (many diffs) |
| 26 | human | 23181 | DIP2A | disco interacting protein 2... | XM_017028299.1 | 45.1% | 41.9% | (many diffs) |
| 27 | mouse | 64451 | Dip2a | disco interacting protein 2... | XR_001779571.1 | 71.6% | (many diffs) | |
| 28 | mouse | 64451 | Dip2a | disco interacting protein 2... | NM_001081419.2 | 42.8% | 41.4% | (many diffs) |
| 29 | mouse | 64451 | Dip2a | disco interacting protein 2... | XM_006513939.3 | 42.5% | 41.1% | (many diffs) |
| 30 | mouse | 64451 | Dip2a | disco interacting protein 2... | XM_006513941.3 | 40.9% | 39% | (many diffs) |
| 31 | mouse | 64451 | Dip2a | disco interacting protein 2... | XM_006513940.3 | 40.5% | 39.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2271
- ORF length:
- 2205
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 tggccatggc tgaccgcggg tgcccgctgg aggcggcgcc gctgcctgcc gaggtgcggg 121 agagcctggc tgagctggag ctggagctgt cggaaggtga catcactcaa aaaggatatg 181 aaaagaaaag ggcaaagctg cttgcacgtt atataccgct tattcaagga atagacccat 241 ctctgcaagc agagaataga attcctgggc cctcacaaac cacggccgct gcacccaagc 301 agcagaagtc tcggcccacc gcctcgaggg atgagcgctt ccggtcagat gtccacactg 361 aagccgtgca agcagctttg gccaaataca aagagaggaa gatgcctatg ccttcgaaga 421 gacgttctgt ccttgtgcat tcgtctgtgg aaacctacac ccctccagac acgtcgtctg 481 cctcagaaga tgagggctct ttacggcgac ccgggcgact cacctccact ccgctccaga 541 gccattccag cgtcgagccc tggctcgacc gggtcattca gggctcgtcc acctcatcct 601 ctgcatcctc cacctcatct cacccgggag ggagacccac cactgctccc agtgctgcag 661 ccacgccggg ggccgccgct accactgcac tcgcaggcct cgaggcccac acccacatag 721 gtgtccctgt gaacagcaga gtgtcctcca aaatccagca gcttctgaac accctgaaga 781 ggccaaagcg ccctccactg aaggagttct ttgtggatga ttttgaggaa ttgttggaag 841 ttcagcaacc agatccaaat cagccaaagc ctgagggaag cgagacgagt gtgctgagag 901 gggagcctct cactgcaggt gtcccccgac cgccgtcgct gttggccacc ttgcagcgct 961 ggggcacaac acagcccaaa tccccctgtc tgactgcctt ggatacaact gggaaagccg 1021 tctacactct cacctatggt aaactttgga gtcggagttt aaaactagct tatactctac 1081 ttaataaact gacaagtaag aatgaacctc tacttaaacc tggagacaga gtggcgctcg 1141 tgtttccgaa tagtgaccct gtgatgttca tggttgcatt ttatgggtgt ctcctggcag 1201 agctggttcc tgtccccata gaagtgccat taacaagaaa ggatgcaggc agccagcagg 1261 ttgggtttct gctgggcagc tgtggagtct tcttggccct gaccacagac gcttgtcaga 1321 aaggcctccc caaggcacag acaggagagg tggcagcttt caaaggttgg cccccgctct 1381 cctggctagt gattgatggg aagcatctag ccaagccccc aaaggactgg caccctctgg 1441 cccaggacac agggactggg actgcctaca ttgagtataa aaccagcaaa gaaggcagta 1501 cggtgggggt cacagtgtcc cacgcatccc tgctggcaca gtgccgggct ctgacccagg 1561 cgtgcgggta ctcagaagct gaaacattaa caaacgtgct ggatttcaaa agggatgctg 1621 gtctgtggca tggcgtgtta acaagcgtca tgaacaggat gcacgtggtc agcgtcccct 1681 acgcgctgat gaaggcgaac ccactctcct ggatccagaa agtgtgcttc tataaagctc 1741 gggccgcgct ggtgaagtcg cgagacatgc actggtctct cctagctcag cggggccaga 1801 gggacgtcag cctcagctca ctgcgcatgc tgattgtggc cgatggtgcc aacccgtggt 1861 cgatctcctc ctgtgacgcc ttcctcaacg tcttccagtc cagaggtctg aggccagagg 1921 tcatctgtcc ttgtgcaagt tctcctgagg cgctgactgt cgccatccgc aggccacctg 1981 atctgggagg accacctcca agaaaagcag tcctgtcgat gaacggtcta agttatggtg 2041 ttatcagagt ggatactgaa gaaaagttgt cagtccttac tgttcaggac gttggtcagg 2101 tgatgcctgg agctaatgta tgtgttgtga agttagaagg taccccttat ctttgtaaaa 2161 ctgatgaagt gggagaaata tgcgtcagtt ccagtgcaac tggcacagcg tactatggat 2221 tgcttggaaT ACAGAGGCAG GTGATGCGCT GCGGACCCCC ACGCCGGGAG TAGATTCCTT 2281 CATTTGCAGC TCGGGAAGTC TTTGTGCAGT AGATGGAAAG CCCAGCAAAC TTGAATTCAC 2341 AAAGCCTAAA AATTGTTGTA CATCAGAAAA CTCCTAAAGT AAAAGGAAAA TATTTGCAGC 2401 AAAAAAAAAA AAAAAGGGCG GCCGCTCTAG AGTATCCCTC GAGGGGCCCA AGCTTACGCG 2461 TACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC 2521 GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG 2581 CTTTATATAT CTTGTGGAAA GGACGATTCG GAAGTCTATG AAGTACAAAT ACGCGTTAAG 2641 TCgacaatca acctctggat tacaaaattt gtgaaagatt