Construct: ORF TRCN0000480527

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014311.2_s317c1
Derived from:
ccsbBroadEn_02730
DNA Barcode:
TTCGGAAGTCTATGAAGTACAAAT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
DIP2A (23181)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000480527
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23181 DIP2A disco interacting protein 2... NM_001146115.2 94.3% 90.7% (many diffs)
2 human 23181 DIP2A disco interacting protein 2... NM_206890.3 89.6% 86% (many diffs)
3 human 23181 DIP2A disco interacting protein 2... NM_206891.3 89.1% 89.1% (many diffs)
4 human 23181 DIP2A disco interacting protein 2... NM_001353944.2 87.3% 82.1% (many diffs)
5 human 23181 DIP2A disco interacting protein 2... XM_011529502.1 85.5% 82.1% (many diffs)
6 human 23181 DIP2A disco interacting protein 2... XM_017028305.1 85.5% 82.1% (many diffs)
7 human 23181 DIP2A disco interacting protein 2... NM_206889.3 84.8% 81.4% (many diffs)
8 human 23181 DIP2A disco interacting protein 2... XM_011529501.1 62.5% 59.8% (many diffs)
9 human 23181 DIP2A disco interacting protein 2... XM_017028304.1 54.8% 52.4% (many diffs)
10 human 23181 DIP2A disco interacting protein 2... XM_017028303.1 51.3% 48.9% (many diffs)
11 human 23181 DIP2A disco interacting protein 2... XM_017028301.1 50.8% 48.5% (many diffs)
12 human 23181 DIP2A disco interacting protein 2... XM_017028300.1 50% 47.7% (many diffs)
13 human 23181 DIP2A disco interacting protein 2... XM_017028298.2 49.6% 47.3% (many diffs)
14 human 23181 DIP2A disco interacting protein 2... XM_017028297.1 48.5% 46.3% (many diffs)
15 human 23181 DIP2A disco interacting protein 2... NM_001353943.1 48.2% 46% (many diffs)
16 human 23181 DIP2A disco interacting protein 2... NM_015151.4 48.2% 46% (many diffs)
17 human 23181 DIP2A disco interacting protein 2... XM_017028295.2 48.2% 46% (many diffs)
18 human 23181 DIP2A disco interacting protein 2... NM_001353942.1 48.2% 46% (many diffs)
19 human 23181 DIP2A disco interacting protein 2... XM_017028294.2 48.2% 46% (many diffs)
20 human 23181 DIP2A disco interacting protein 2... NM_001146116.1 48% 45.8% (many diffs)
21 human 23181 DIP2A disco interacting protein 2... XM_017028293.1 47.2% 45% (many diffs)
22 human 23181 DIP2A disco interacting protein 2... XM_011529492.2 47.2% 45% (many diffs)
23 human 23181 DIP2A disco interacting protein 2... XM_017028291.1 47.2% 45% (many diffs)
24 human 23181 DIP2A disco interacting protein 2... XM_017028292.1 47.2% 45% (many diffs)
25 human 23181 DIP2A disco interacting protein 2... XM_017028302.2 46.1% 42.9% (many diffs)
26 human 23181 DIP2A disco interacting protein 2... XM_017028299.1 45.1% 41.9% (many diffs)
27 mouse 64451 Dip2a disco interacting protein 2... XR_001779571.1 71.6% (many diffs)
28 mouse 64451 Dip2a disco interacting protein 2... NM_001081419.2 42.8% 41.4% (many diffs)
29 mouse 64451 Dip2a disco interacting protein 2... XM_006513939.3 42.5% 41.1% (many diffs)
30 mouse 64451 Dip2a disco interacting protein 2... XM_006513941.3 40.9% 39% (many diffs)
31 mouse 64451 Dip2a disco interacting protein 2... XM_006513940.3 40.5% 39.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2271
ORF length:
2205
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61tggccatggc tgaccgcggg tgcccgctgg aggcggcgcc gctgcctgcc gaggtgcggg
121agagcctggc tgagctggag ctggagctgt cggaaggtga catcactcaa aaaggatatg
181aaaagaaaag ggcaaagctg cttgcacgtt atataccgct tattcaagga atagacccat
241ctctgcaagc agagaataga attcctgggc cctcacaaac cacggccgct gcacccaagc
301agcagaagtc tcggcccacc gcctcgaggg atgagcgctt ccggtcagat gtccacactg
361aagccgtgca agcagctttg gccaaataca aagagaggaa gatgcctatg ccttcgaaga
421gacgttctgt ccttgtgcat tcgtctgtgg aaacctacac ccctccagac acgtcgtctg
481cctcagaaga tgagggctct ttacggcgac ccgggcgact cacctccact ccgctccaga
541gccattccag cgtcgagccc tggctcgacc gggtcattca gggctcgtcc acctcatcct
601ctgcatcctc cacctcatct cacccgggag ggagacccac cactgctccc agtgctgcag
661ccacgccggg ggccgccgct accactgcac tcgcaggcct cgaggcccac acccacatag
721gtgtccctgt gaacagcaga gtgtcctcca aaatccagca gcttctgaac accctgaaga
781ggccaaagcg ccctccactg aaggagttct ttgtggatga ttttgaggaa ttgttggaag
841ttcagcaacc agatccaaat cagccaaagc ctgagggaag cgagacgagt gtgctgagag
901gggagcctct cactgcaggt gtcccccgac cgccgtcgct gttggccacc ttgcagcgct
961ggggcacaac acagcccaaa tccccctgtc tgactgcctt ggatacaact gggaaagccg
1021tctacactct cacctatggt aaactttgga gtcggagttt aaaactagct tatactctac
1081ttaataaact gacaagtaag aatgaacctc tacttaaacc tggagacaga gtggcgctcg
1141tgtttccgaa tagtgaccct gtgatgttca tggttgcatt ttatgggtgt ctcctggcag
1201agctggttcc tgtccccata gaagtgccat taacaagaaa ggatgcaggc agccagcagg
1261ttgggtttct gctgggcagc tgtggagtct tcttggccct gaccacagac gcttgtcaga
1321aaggcctccc caaggcacag acaggagagg tggcagcttt caaaggttgg cccccgctct
1381cctggctagt gattgatggg aagcatctag ccaagccccc aaaggactgg caccctctgg
1441cccaggacac agggactggg actgcctaca ttgagtataa aaccagcaaa gaaggcagta
1501cggtgggggt cacagtgtcc cacgcatccc tgctggcaca gtgccgggct ctgacccagg
1561cgtgcgggta ctcagaagct gaaacattaa caaacgtgct ggatttcaaa agggatgctg
1621gtctgtggca tggcgtgtta acaagcgtca tgaacaggat gcacgtggtc agcgtcccct
1681acgcgctgat gaaggcgaac ccactctcct ggatccagaa agtgtgcttc tataaagctc
1741gggccgcgct ggtgaagtcg cgagacatgc actggtctct cctagctcag cggggccaga
1801gggacgtcag cctcagctca ctgcgcatgc tgattgtggc cgatggtgcc aacccgtggt
1861cgatctcctc ctgtgacgcc ttcctcaacg tcttccagtc cagaggtctg aggccagagg
1921tcatctgtcc ttgtgcaagt tctcctgagg cgctgactgt cgccatccgc aggccacctg
1981atctgggagg accacctcca agaaaagcag tcctgtcgat gaacggtcta agttatggtg
2041ttatcagagt ggatactgaa gaaaagttgt cagtccttac tgttcaggac gttggtcagg
2101tgatgcctgg agctaatgta tgtgttgtga agttagaagg taccccttat ctttgtaaaa
2161ctgatgaagt gggagaaata tgcgtcagtt ccagtgcaac tggcacagcg tactatggat
2221tgcttggaaT ACAGAGGCAG GTGATGCGCT GCGGACCCCC ACGCCGGGAG TAGATTCCTT
2281CATTTGCAGC TCGGGAAGTC TTTGTGCAGT AGATGGAAAG CCCAGCAAAC TTGAATTCAC
2341AAAGCCTAAA AATTGTTGTA CATCAGAAAA CTCCTAAAGT AAAAGGAAAA TATTTGCAGC
2401AAAAAAAAAA AAAAAGGGCG GCCGCTCTAG AGTATCCCTC GAGGGGCCCA AGCTTACGCG
2461TACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC
2521GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG
2581CTTTATATAT CTTGTGGAAA GGACGATTCG GAAGTCTATG AAGTACAAAT ACGCGTTAAG
2641TCgacaatca acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)

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