Construct: ORF TRCN0000480569
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006226.1_s317c1
- Derived from:
- ccsbBroadEn_01010
- DNA Barcode:
- AACCCTTTCACATTTGCCTTCATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MGST2 (4258)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480569
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4258 | MGST2 | microsomal glutathione S-tr... | NM_001204366.1 | 100% | 100% | |
| 2 | human | 4258 | MGST2 | microsomal glutathione S-tr... | NM_002413.5 | 100% | 100% | |
| 3 | human | 4258 | MGST2 | microsomal glutathione S-tr... | XM_017008211.2 | 100% | 100% | |
| 4 | human | 4258 | MGST2 | microsomal glutathione S-tr... | XM_017008212.2 | 100% | 100% | |
| 5 | human | 4258 | MGST2 | microsomal glutathione S-tr... | XM_017008213.1 | 100% | 100% | |
| 6 | human | 4258 | MGST2 | microsomal glutathione S-tr... | NM_001204367.1 | 53.7% | 53.7% | 0_1ins204 |
| 7 | human | 4258 | MGST2 | microsomal glutathione S-tr... | XM_006714221.2 | 53.7% | 53.7% | 0_1ins204 |
| 8 | human | 4258 | MGST2 | microsomal glutathione S-tr... | XM_011531972.2 | 53.7% | 53.7% | 0_1ins204 |
| 9 | human | 4258 | MGST2 | microsomal glutathione S-tr... | XM_017008214.1 | 53.7% | 53.7% | 0_1ins204 |
| 10 | human | 4258 | MGST2 | microsomal glutathione S-tr... | NM_001204368.2 | 52.3% | 37.5% | 158_159ins71;231_232ins139 |
| 11 | human | 4258 | MGST2 | microsomal glutathione S-tr... | XR_001741221.1 | 24.1% | 1_295del;453_454ins71;666_1464del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 507
- ORF length:
- 441
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cgggaactcg atcctgctgg ctgctgtctc tattctctcg gcctgtcagc 121 aaagttattt tgctttgcaa gttGGAAAGG CAAGATTAAA ATACAAAGTT ACGCCCCCAG 181 CAGTCACTGG GTCACCAGAG TTTGAGAGAG TATTTCGGGC ACAACAAAAC TGTGTGGAGT 241 TTTATCCTAT ATTCATAATT ACATTGTGGA TGGCTGGGTG GTATTTCAAC CAAGTTTTTG 301 CTACTTGTCT GGGTCTGGTG TACATATATG GCCGTCACCT ATACTTCTGG GGATATTCAG 361 AAGCTGCTAA AAAACGGATC ACCGGTTTCC GACTGAGTCT GGGGATTTTG GCCTTGTTGA 421 CCCTCCTAGG TGCCCTGGGA ATTGCAAACA GCTTTCTGGA TGAATATCTG GACCTCAATA 481 TTGCCAAGAA ACTGAGGCGG CAATTCTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 541 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 601 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAACCCTTT 661 CACATTTGCC TTCATCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 721 aagatt