Construct: ORF TRCN0000480609
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003878.1_s317c1
- Derived from:
- ccsbBroadEn_14391
- DNA Barcode:
- CACTGCCAGTATACCGGTAATTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLEKHA7 (144100)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480609
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 144100 | PLEKHA7 | pleckstrin homology domain ... | NM_001329631.2 | 66.5% | 66.5% | (many diffs) |
| 2 | human | 144100 | PLEKHA7 | pleckstrin homology domain ... | NM_175058.5 | 66.4% | 66.4% | (many diffs) |
| 3 | human | 144100 | PLEKHA7 | pleckstrin homology domain ... | XM_017017242.2 | 65% | 65% | (many diffs) |
| 4 | human | 144100 | PLEKHA7 | pleckstrin homology domain ... | XM_017017241.2 | 62.8% | 62.8% | (many diffs) |
| 5 | human | 144100 | PLEKHA7 | pleckstrin homology domain ... | XM_024448366.1 | 59.1% | 59.1% | (many diffs) |
| 6 | human | 144100 | PLEKHA7 | pleckstrin homology domain ... | XM_024448367.1 | 59.1% | 59.1% | (many diffs) |
| 7 | human | 144100 | PLEKHA7 | pleckstrin homology domain ... | XM_024448368.1 | 59.1% | 59.1% | (many diffs) |
| 8 | human | 144100 | PLEKHA7 | pleckstrin homology domain ... | XM_024448369.1 | 59.1% | 59.1% | (many diffs) |
| 9 | human | 144100 | PLEKHA7 | pleckstrin homology domain ... | XM_024448365.1 | 58.6% | 58.6% | (many diffs) |
| 10 | human | 144100 | PLEKHA7 | pleckstrin homology domain ... | XM_024448364.1 | 58.5% | 58.5% | (many diffs) |
| 11 | human | 144100 | PLEKHA7 | pleckstrin homology domain ... | NM_001329630.2 | 58.4% | 58.4% | (many diffs) |
| 12 | human | 144100 | PLEKHA7 | pleckstrin homology domain ... | XM_024448363.1 | 57.4% | 57.3% | (many diffs) |
| 13 | human | 144100 | PLEKHA7 | pleckstrin homology domain ... | XM_024448362.1 | 57% | 57% | (many diffs) |
| 14 | human | 144100 | PLEKHA7 | pleckstrin homology domain ... | XM_024448361.1 | 56.4% | 56.4% | (many diffs) |
| 15 | human | 144100 | PLEKHA7 | pleckstrin homology domain ... | XM_024448360.1 | 56.2% | 56.2% | (many diffs) |
| 16 | human | 144100 | PLEKHA7 | pleckstrin homology domain ... | XM_024448359.1 | 55.6% | 55.6% | (many diffs) |
| 17 | human | 144100 | PLEKHA7 | pleckstrin homology domain ... | XM_024448358.1 | 55.6% | 55.6% | (many diffs) |
| 18 | human | 144100 | PLEKHA7 | pleckstrin homology domain ... | XM_024448356.1 | 54.5% | 54.5% | (many diffs) |
| 19 | human | 144100 | PLEKHA7 | pleckstrin homology domain ... | XM_024448357.1 | 54.5% | 54.5% | (many diffs) |
| 20 | human | 144100 | PLEKHA7 | pleckstrin homology domain ... | XM_024448370.1 | 46.3% | 46.3% | (many diffs) |
| 21 | human | 144100 | PLEKHA7 | pleckstrin homology domain ... | XR_002957126.1 | 38.1% | (many diffs) | |
| 22 | mouse | 233765 | Plekha7 | pleckstrin homology domain ... | NM_001305189.1 | 63.3% | 66.7% | (many diffs) |
| 23 | mouse | 233765 | Plekha7 | pleckstrin homology domain ... | NM_172743.3 | 62.1% | 65.4% | (many diffs) |
| 24 | mouse | 233765 | Plekha7 | pleckstrin homology domain ... | XM_017322197.1 | 60.9% | 64.2% | (many diffs) |
| 25 | mouse | 233765 | Plekha7 | pleckstrin homology domain ... | NM_001305190.1 | 60.7% | 64% | (many diffs) |
| 26 | mouse | 233765 | Plekha7 | pleckstrin homology domain ... | XM_017322196.1 | 59.4% | 62.6% | (many diffs) |
| 27 | mouse | 233765 | Plekha7 | pleckstrin homology domain ... | XM_017322193.1 | 55.1% | 58% | (many diffs) |
| 28 | mouse | 233765 | Plekha7 | pleckstrin homology domain ... | XM_017322191.1 | 53.9% | 56.7% | (many diffs) |
| 29 | mouse | 233765 | Plekha7 | pleckstrin homology domain ... | XM_017322189.1 | 52.7% | 55.5% | (many diffs) |
| 30 | mouse | 233765 | Plekha7 | pleckstrin homology domain ... | NM_001305186.1 | 52.5% | 55.2% | (many diffs) |
| 31 | mouse | 233765 | Plekha7 | pleckstrin homology domain ... | XM_017322188.1 | 52.2% | 54.9% | (many diffs) |
| 32 | mouse | 233765 | Plekha7 | pleckstrin homology domain ... | XM_017322187.1 | 52.1% | 54.9% | (many diffs) |
| 33 | mouse | 233765 | Plekha7 | pleckstrin homology domain ... | XM_017322185.1 | 51% | 53.7% | (many diffs) |
| 34 | mouse | 233765 | Plekha7 | pleckstrin homology domain ... | XM_017322194.1 | 51% | 53.7% | (many diffs) |
| 35 | mouse | 233765 | Plekha7 | pleckstrin homology domain ... | XM_017322195.1 | 51% | 53.7% | (many diffs) |
| 36 | mouse | 233765 | Plekha7 | pleckstrin homology domain ... | NM_001305185.1 | 50.6% | 53.2% | (many diffs) |
| 37 | mouse | 233765 | Plekha7 | pleckstrin homology domain ... | XM_017322192.1 | 49.3% | 52% | (many diffs) |
| 38 | mouse | 233765 | Plekha7 | pleckstrin homology domain ... | XM_017322190.1 | 48.7% | 51.3% | (many diffs) |
| 39 | mouse | 233765 | Plekha7 | pleckstrin homology domain ... | XR_001785538.1 | 47.5% | (many diffs) | |
| 40 | mouse | 233765 | Plekha7 | pleckstrin homology domain ... | XM_017322186.1 | 47.1% | 49.6% | (many diffs) |
| 41 | mouse | 233765 | Plekha7 | pleckstrin homology domain ... | XM_017322198.1 | 45.3% | 43.9% | (many diffs) |
| 42 | mouse | 233765 | Plekha7 | pleckstrin homology domain ... | XR_001785539.1 | 34.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2313
- ORF length:
- 2244
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggatttaccc actggcccaa gaggccagca ggcacagccc caacgggcag 121 agaagaatgg aatgctgcct gcctcatatg gcccaggaga acagaatggg actggtgggt 181 accagcgggc ctttcctccc aggaccaacc ccgaaaaaca cagccaaagg aagagcaatc 241 tggcccaggt ggagcactgg gcaagggccc agaaagggga tagcaggagt cttcccttgg 301 accagacgct tcctcgccag ggtcctggcc aatccctgtc cttcccagaa aactaccaga 361 ctcttcccaa tagcacccga cacccctcgg ggggctcctc gccacctccc cgaaacctgc 421 caagtgacta caagtatgcg caggaccgag ccagccacct gaagatgtcg agtgaagagc 481 gccgggcgca ccgggatggc accgtgtggc agctctacga gtggcagcag cgccagcagt 541 tccggcacgg cagccccaca gcgcccatct gccttggctc cccagagttc accgaccagg 601 gccggagcag gagcatgcta gaggtgcccc gctccatctc tgtgcctcca tctccctcgg 661 acatccctcc cccaggaccc ccaagggtct tcccaccccg gcggccacac acaccagcag 721 agcgagtcac agtgaagcca ccggaccaga ggaggagtgt ggacatctcg ctgggggatt 781 ctccaaggag ggcacggggc cacgctgtca agaactcatc tcatgtggac cgacgctcca 841 tgccctccat gggttacatg acccacaccg tcagcgctcc cagtttacat ggaaaatcgg 901 ctgatgatac ctacctccag ctgaagaaag acctggagta cctggatcta aagatgacag 961 gccgggacct tctcaaggat cgaagtctga agcctgtgaa gatcgctgag agcgacactg 1021 acgtcaaact gagcatcttc tgtgaacaag acagggtcct ccaggacttg gaagacaaga 1081 tacgagccct taaagagaac aaagaccagc tagaatctgt gctggaggtg ttgcacagac 1141 agatggagca gtaccgagac cagccccagc acttggagaa gattgcctac cagcagaagt 1201 tgctgcagga ggaccttgtc catatccgag ctgagctctc cagagagtcc actgagatgg 1261 aaaatgcttg gaacgaatac ctgaagttgg agaatgatgt ggaacagctg aagcagaccc 1321 tgcaggagca acacagaaga gccttttttt tccaggagaa atcgcagata cagaaagatc 1381 tatggagaat tgaagatgtc actgcaggcc tgagtgcaaa taaagagaac ttcagaattc 1441 tagtggagtc agtaaaaaat ccggagagaa aaacggtgcc tttgtttcct cacccgcctg 1501 tgccttcact ctcaacttct gagagcaagc cgcccccaca gcccagtcct cccaccagcc 1561 ctgtgcggac ccctctggag gttcgactct tcccccagct gcaaacctac gtgccgtacc 1621 gacctcaccc accccagctg aggaaagtga catcccccct tcagtcacca actaaggcga 1681 agcccaaagt tcaggaagat gaagcacctc ccaggccccc actccccgaa ctctacagcc 1741 cagaggacca gccccctgct gtgccgcctc tgccaagaga ggccaccatc atccggcaca 1801 catctgtgcg gggcctcaag cggcagtcag acgagaggaa gcgagaccgg gagctggggc 1861 agtgtgtgaa tggggattcc agggtggagc tgcggtcgta tgtcagtgag cctgagctgg 1921 cgacccTCAG CGGGGACATG GCCCAGCCCT CCCTAGGACT TGTGGGCCCT GAGAGCAGGT 1981 ACCAGACGCT GCCAGGCAGA GGGCTCTCAG GGTCCACGTC AAGGCTCCAG CAGTCGTCCA 2041 CCATTGCTCC CTACGTCACA CTCCGGAGGG GTCTCAATGC CGAAAGCAGC AAGGCGACCT 2101 TCCCTAGACC TAAGAGTGCC TTGGAGCGCC TGTACTCAGG GGATCACCAG CGAGGCAAGA 2161 TGAGTGCAGA GGAGCAGCTG GAGCGCATGA AGCGACACCA GAAGGCCCTG GTCCGAGAGC 2221 GCAAGAGGAC ACTGGGCCAA GGGGAGAGGA CGGGCCTGCC CTCATCTCGC TACCTCAGCC 2281 GGCCGCTCCC TGGAGATCTT GGCTCAGTAT GTTTGCCAAC TTTCTTGTAC AAAGTGGTTG 2341 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2401 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACA 2461 CTGCCAGTAT ACCGGTAATT GTACGCGTTA AGTCgacaat caacctctgg attacaaaat 2521 ttgtgaaaga tt